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Subject: Veterinary Medicine × Author: DEVARAJA, K. × End date: 2018 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    STANDARDIZATION OF A FIELD TEST FOR THE DIAGNOSIS OF ANTHRAX IN LIVESTOCK
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-11) DEVARAJA, K.; Dr. P.T. RAMESH
    The present study reports the isolation and characterization of Bacillus anthracis and standardization of a field test for the diagnosis of anthrax in livestock. Thirteen isolates were recovered from seventeen carcasses, suspected of anthrax. Colony morphology showed typical ‘curled hair’ or ‘medusa head’ appearance. Gram’s staining revealed G
  • Thumbnail Image
    ThesisItemOpen Access
    STANDARDIZATION OF A FIELD TEST FOR THE DIAGNOSIS OF ANTHRAX IN LIVESTOCK
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-11) DEVARAJA, K.; Dr. P.T. RAMESH
    The present study reports the isolation and characterization of Bacillus anthracis and standardization of a field test for the diagnosis of anthrax in livestock. Thirteen isolates were recovered from seventeen carcasses, suspected of anthrax. Colony morphology showed typical ‘curled hair’ or ‘medusa head’ appearance. Gram’s staining revealed Gram- positive rods which appeared in pairs or short chains, giving a ‘box car’ or ‘jointed bamboo-rod’ appearance. All the isolates were positive to Voges- Proskauer and nitrate reduction tests, non haemolytic on blood agar, did not grow on Mac Conkey agar and were sensitive to penicillin. All thirteen isolates yielded amplified products of 596 bp specific for pag gene and 846 bp amplicon specific for cap gene using PCR. Latex agglutination test was standardized using polyclonal antibodies. The LAT was positive B. anthracis field isolates (+++). The diagnostic sensitivity revealed that a minimum of 2.15×104 CFU/ml of B. anthracis bacilli was adequate to elicit a definite agglutination. Further the diagnostic specificity of LAT was so efficient that, it did not produce any false positive reactions with eleven other bacterial species tested even at 1000 times more bacterial cells than the B. anthracis. So the standardization of LAT for the rapid, sensitive and specific identification of B. anthracis is an invaluable tool for the field diagnosis of anthrax.