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ThesisItem Open Access STUDIES ON EFFECT OF INTRAUTERINE THERAPY WITH OZONATED OILS AS A PROPHYLACTIC APPROACH FOR POSTPARTUM UTERINE INFECTIONS IN CROSSBRED DAIRY COWS(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2021-01) CHIRANTH, J. P.; NARASIMHA MURTHYThirty six normally calved crossbred dairy cows were randomly divided into 3 groups with 12 each to evaluate the prophylactic efficacy of intrauterine ozonated oil on uterine involution pattern, uterine bacterial load reduction, postpartum uterine infections and their subsequent reproductive performance. Group I served as control and Group II and III received one time and two time intrauterine ozonated oil (100 mL) on day 5-7 and 14, respectively. Uterine involution pattern and uterine aerobic bacterial studies were done on day 7, 14, 21, 28 and 35 pp. All the animals were screened for postpartum uterine infections using vaginoscopy and endometrial cytology and their reproductive performance were recorded. Observations recorded from uterine involution assessment and total uterine bacterial count revealed that reduction rate of uterine size and bacterial load was similar in both control and preventive ozone infused groups. Following intrauterine administration of ozonated oils, none of the study cows were found positive for puerperal metritis on day 10-14 pp. However, incidence of clinical endometritis examined on day 21(Group I vs II vs III; 33.33 vs 25 vs 25%) and sub clinical endometritis on day 28 pp (Group I vs II vs III; 25 vs 25 vs 16.6%) and on day of subsequent estrus (Group I vs II vs III; 16.6 vs 16.6 vs 8.3%) were noticed. In conclusion, the present study revealed that the prophylactic effect of intrauterine ozonated oil on uterine involution, reproductive performances and in preventing postpartum uterine infections is non-significant among different study groups. Keywords: Intrauterine ozonated oil, Dairy cows, Postpartum uterine infections, Prophylactic effectThesisItem Open Access AUGMENTATION OF FERTILITY BY HERBAL AND HORMONAL PROTOCOLS IN POSTPARTUM ANESTRUS COWS(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2021-03) MOHD MUJAHEED PASHA; R. G. BIJURKARThe present study evaluated the fertility response and serum profile changes in progesterone and biochemical constituents in 18 postpartum anestrus (>60 days) cows, treated under field conditions with Estrona feeding at 125gm/day/animal for four days (group II; n=6) or CIDR-Synch protocol (group III; n=6) and an untreated Control (group I; n=6). The animals were observed for estrus signs and inseminated. The estrus induction rates in group I, II and III were 16.66, 66.66 and 100.00 per cent with conception rates of 0.00, 50.00 and 66.66 per cent, respectively. The mean serum progesterone concentration (ng/mL) measured before (0th day) and after (30th day) treatment increased nonsignificantly (P<0.05) in group II and significantly (P<0.05) in group III, when compared between the groups, group I had significantly low progesterone on 30th day of treatment compared to other two groups. The overall mean calcium and phosphorus concentration (mg/dL) varied significantly (P<0.05) within group II and mean glucose concentration varied significantly (P<0.05) in group III. However, no-significant difference was observed in cholesterol concentration observed in groups I, II and III, before (0th day) and after (30th day) treatment. To conclude, above results indicates that the feeding of Estrona powder and progesterone-based CIDR-Synch protocol improved estrus induction and conception rates in postpartum anestrus cows. Key words: CIDR-Synch, Estrus induction, Estrona powder and Postpartum anestrus cowsThesisItem Open Access DOPPLER ULTRASONOGRAPHIC ASSESSMENT OF UTERINE ARTERY BLOOD FLOW DURING NORMAL PREGNANCY IN LABRADOR RETRIEVER(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2019-08) NANDITHA, H. J.; Dr. NARASIMHA MURTHYThe aim of this study was to record the blood flow characteristics of uterine artery using Doppler ultrasonography throughout the normal gestation of Labrador retriever dogs. Ten healthy Labrador female dogs aged between 2-5 years and parity 1-3 were ultrasonographically assessed during the gestation from day of first breeding until day 60 at an interval of 10 days. Colour Doppler was used to locate both the uterine arteries on either sides of the body and Pulsed wave Doppler was performed to obtain the parameters such as Peak Systolic Velocity (PSV), End Diastolic Velocity (EDV), Pulsatality Index (PI), Resistive Index (RI) and S/D ratio. The study also recorded the litter size and nature of parturition. The obtained DopplThesisItem Open Access ULTRASONOGRAPHIC ASSESSMENT OF PLACENTAL THICKNESS IN DOGS AND ITS RELATIONSHIP WITH GESTATIONAL AGE(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-08) CHANDRASHEKHAR AYYA SALIMATH; Dr. G. SUDHAThe measurement of gestational sac diameter (GSD) and biparietal diameter (BPD) using ultrasonography (USG) remained fairly accurate determinants of gestational age (GA) in canines during early and later stages of gestation with precision of ±1 to ± 2 days. The present study looked at the possibility of placental thickness (PT) as a determinant of GA using USG in normal pregnancies of different breeds and body sizes and then to correlate the same with reported gestational age (RGA). A cross sectional observational study was conducted on 90 pregnant dogs of different breeds and parity and a total of 112 observations were made by using ALOKA PRO SOUND α-6 ultra sound unit using 5 MHz convex probe.ThesisItem Open Access EFFECT OF SUPPLEMENTATION OF SAFFLOWER OIL IN OVSYNCH ON FERTILITY OF POSTPARTUM BIDRI GOATS(KARNATAKA VETERINARY, ANIMAL AND FOISHERIE'S SCIENCES UNIVERSITY, BIDAR, 2020-01) RAVIKIRAN, S.; Dr. R G. BIJURKARThe study was conducted to find out the effect of safflower oil supplementation combined with ovsynch estrus synchronization protocol in postpartum Bidri goats. Twenty four full term pregnant goats were selected and divided into 3 groups (n=8). From day of kidding, all the 3 groups were fed with concentrate feed (250 gm/day/doe) for 30 days. Group one (T0) was kept control and maintained on concentrate feed without additional supplementation. Treatment groups – Group 2 (T1) and Group 3 (T2) received safflower oil supplementation at the rate of 30 gm and 50 gm/day/doe respectively, for 30 days. Ovsynch (G-P-G) estrous synchronization protocol was carried in all 3 groups from day 16 postpartum. All animals received a I/M inj of GnRH @ 4 mcg on day 16 post kidding and I/M inj of PGF2α @ 250 mg on day 23; Upon observing of estrus signs, does were allowed for natural mating. All the animals received second I/M inj of GnRH @ 4 mcg on day 25 (48 hours after PGF2α injection). Estrus induction rates for all the 3 groups were 100 %. Conception rates for T0, T1 and T2 were 50%, 75% and 100% respectively. Mean ± SE values of onset of estrus for Group T0, T1 and T2 were found to be 62.25±1.66, 49.625±1.48 and 41.25 ± 1.71 hr respectively. Mean ± SE values of duration of estrus for Group T0, T1 and T2 were found to be 38.25±1.21, 46.125±0.89 and 42.75±3.50 hr respectively. Serum samples were estimated for progesterone, glucose and cholesterol levels. Based on conception rate, it was noticed that safflower oil supplementation augmented conception rates in both Group 2 (T1) and Group 3 (T2). It was concluded that Group 3, which received 50 gram safflower oil + Ovsynch had better efficiency in increasing conception rate of postpartum Bidri does.ThesisItem Open Access CLINICAL EFFICACY OF MIFEPRISTONE AND MISOPROSTOL IN INDUCTION OF PARTURITION IN FEMALE DOGS WITH SINGLE PUPPY SYNDROME(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR- 585 401, 2018-02) VIJAY PEETALA; Dr. V. CHANDRASHEKHARA MURTHYA clinical study was designed to evaluate the efficacy of mifepristone and misoprostol in induction of parturition in dogs with single puppy syndrome. The incidence of single puppy syndrome was 5.08 per cent, recorded in nine different breeds of dogs. The highest incidence was observed in Pug while the lowest in Dachshund and Chihuahua. Further, higher incidence was noticed in primiparous bitches with less than two years of age. The incidence of dystocia due to single puppy syndrome was 25 per cent. The blood glucose, serum calcium and phosphorus concentrations were within normal physiological range in both control and treatment groups. In control group, all the animals bearing singleton were presented with dystocia and assisted whelping was performed. All the animals in treatment group hadn’t shown any signs of parturition even after 62 days of gestation were administered mifepristone @ 5 mg/kg BW per orally twice a day at 12 hours interval followed by intra vaginal administration of misoprostol @ 200 µg for bitches with ≤ 20 kg BW and 400 µg for bitches with > 20 kg BW, 12 hours after second dose of mifepristone. The mean time taken for the expulsion of the single pup from the time of initiation of treatment was 28 ± 2.74 hours and the mean time taken for the expulsion of the single pup after administering misoprostol intravaginally was 5.3 ± 1.7 hours. Live singletons were whelped in all treatment group animals giving 100 per cent results. It is concluded that the use of mifepristone and misoprostol combination is safe and effective for induction of parturition in female dogs with single puppy syndromeThesisItem Open Access CLINICAL STUDIES ON PSEUDOPREGNANCY IN DOGS(KARNATAKA VETERINARY, ANIMAL AND FISCHERIES SCIENCE UNIVERSITY, BIDAR, 2018-08) SHILPA V. S.; Dr. V. CHANDRASHEKHARA MURTHYA study on the prevalence, predisposing factors, clinical signs associated with pseudopregnancy and efficiency of dopamine agonists and management practices to reduce the clinical manifestation of pseudopregnancy was conducted. The prevalence of pseudopregnancy was 35.41% with highest prevalence in medium breeds and lowest in small breeds. The prevalence was higher in young (less than 2 years) and nulliparous female dogs. Age and parity showed a statistically significant influence on the exhibition of pseudopregnancy. The signs were exhibited during the anestrus stage of estrous cycle in majority of dogs as indicated by the serum progesterone values less than 1 ng/ml. The average number of days from last estrus was 63±2.25 days. The clinical signs varied widely between individuals and mammary development with milk or milk like secretion was the most common sign (96.08%). Physical changes and maternal instincts were also recorded and clinical signs were seen in less than 50% of the dogs. The complications like mastitis or mammary dermatitis were rare (3.96%). Cabergoline at 5µg/Kg BW once daily took an average of 6.4±0.65 days for complete recovery and bromocriptine at 10µg/Kg BW twice daily took an average of 9.5±0.73 days. Managemental practices like food and water withdrawal during night hours and application of Elizabethan collars for an average of 15±0.77 days also had a significant effect in reducing clinical signs compared to control group. Even though pseudopregnancy is a self-limiting condition, when clinical signs are exacerbated, compared to bromocriptine, cabergoline was found to be safe and effective. Managemental interventions were found to be effective in absence of veterinary aid.ThesisItem Open Access EFFECT OF SUPPLEMENTATION OF GLUTATHIONE AND HONEY IN SKIM MILK BASED EXTENDERS FOR PRESERVATION OF BOER BUCK SEMEN AT REFRIGERATION TEMPERATURE(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2019-12) SURESH ARAKERI; Prof. M. K. TANDLEAddition of 1% honey in skim milk glutathione extender increased motile sperm percentage while addition of 1% honey in skim milk extender reduced it. Addition of 1% honey in skim milk glutathione extender reduced immotile sperm percentage in contrast addition of 2% honey in skim milk extender increased it. Addition of 1% honey in skim milk glutathione extender improved rapid progressive sperm percentage whereas addition of 2% honey in skim milk extenders reduced it. Slow progressive sperm percentage was lower in skim milk glutathione extender whereas addition of 2% honey in skim milk extender increased it. Non-progressive sperm percentage was lower in skim milk glutathione extender however addition of 1% honey in it increased it. Addition of 1% honey in skim milk glutathione extender showed higher sperm curvilinear velocity, sperm straight line velocity and sperm average path velocity however addition of 2% honey in skim milk extender reduced them. Addition of 1% honey in skim milk extender reduced sperm linearity percentage, sperm wobble percentage and sperm straightness percentage whereas the same were higher in skim milk glutathione extender. Addition of honey (1% or 2%) did not show effect on sperm beat cross frequency and amplitude of lateral sperm head displacement whereas the same were higher in skim milk glutathione extender and lower in skim milk extender. Addition of 1% honey in skim milk glutathione extender increased sperm dance whereas 2% honey addition in skim milk extender lowered it. Addition of 2% honey in skim milk extender increased sperm dance mean whereas reduced in skim milk glutathione extender at 72 h of refrigeration.ThesisItem Open Access EFFECT OF SUPPLEMENTATION OF GLUTATHIONE AND α-TOCOPHEROL IN TRIS AND SKIM MILK BASED EXTENDERS FOR PRESERVATION OF RAM SEMEN AT REFRIGERATION TEMPERATURE(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2019-11) SANGAMESH MUNIYAPPANAVAR; Prof. M. K. TANDLEThe research findings of the present study are that addition of glutathione improved motile sperm and reduced immotile sperm percentage than α-tocopherol in skim milk than TRIS egg yolk based extenders. Addition of glutathione improved rapid progressive sperm percentage than α-tocopherol in TRIS egg yolk than skim milk based extenders. Addition of α-tocopherol in TRIS egg yolk reduced slow progressive sperm percentage whereas improved in skim milk based extenders as compared to addition of glutathione. Addition of either glutathione or α-tocopherol did not reduce the non-progressive sperm percentage in skim milk and TRIS egg yolk based extenders. Addition of glutathione improved sperm curvilinear velocity, sperm average path velocity and sperm straight line velocity in TRIS egg yolk based extenders whereas α-tocopherol reduced it in skim milk based extenders. Addition of glutathione did not improve sperm straightness percentage in skim milk and TRIS egg yolk based extenders however addition of α-tocopherol reduced it in TRIS egg yolk than skim milk based extenders. Addition of α-tocopherol reduced sperm wobble percentage in skim milk than TRIS egg yolk based extenders at refrigeration however glutathione has shown no effect on it. Addition of glutathione in skim milk based extenders improved sperm beat cross frequency, sperm dance and sperm dance mean whereas addition of α-tocopherol in TRIS egg yolk extender reduced it. There was no effect of addition of glutathione on amplitude of lateral sperm head displacement in skim milk based extender however addition of α-tocopherol improved it at 72 h of refrigeration in NARI Suwarna rams.
