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2018 - 20192
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Subject: Livestock Product Technology × End date: 2019 × Start date: 2018 × Type: Thesis × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    ISOLATION AND MOLECULAR CHARACTERIZATION OF ANTIMICROBIAL RESISTANT E. COLI IN BROILER VALUE CHAIN
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2019-08) NIVEDITHA, S. N; r. Md. NADEEM FAIROZE
    The present investigation was carried out to establish the prevalence of E. coli in broiler production chain to document their antimicrobial resistance. A total of 243 samples were collected from nine (9) randomly selected commercial broiler farms on day 0 and on day 32-35. The overall prevalence of E. coli in the present study was 57.61 per cent (140/243). There was no significant difference in prevalence of E. coli in categories of farms, wh
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    ThesisItemOpen Access
    PREVALENCE OF EXTENDED SPECTRUM BETA LACTAMASE- ESCHERICHIA COLI IN RETAIL CHICKEN MEAT IN BENGALURU
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR – 585401, 2018-07) RAJESH, S.; Dr. Md. NADEEM FAIROZE
    The present investigation was undertaken to study the prevalence and distribution of E. coli and their antimicrobial resistance with special reference to extended spectrum βlactamases (ESBLs) in 100 chicken retail outlets in Bengaluru. A total of 400 samples were tested and the overall prevalence of E. coli in the present study was 57.25 per cent (229/400). A highly significant (P≤ 0.01) difference in the prevalence was observed among the different samples obtained from the retail outlets. All the isolates of E. coli were confirmed by PCR targeting uspA gene. The overall prevalence of Extended Spectrum Betalactamase (ESBL) E. coli in different samples was 46.25 per cent, whereas the overall prevalence of ESBL in E. coli was 80.78 per cent. Molecular and genotypic characterization revealed that blaTEM gene was carried by 77.83 per cent of the isolates followed by blaCTX-M gene in 31.35 per cent and blaSHV in 9.19 per cent isolates either alone or in combination. The antibiotic susceptibility pattern of ESBL E. coli revealed that all the isolates were resistant to Erythromycin and Penicillin (100 %) followed by Ampicillin, Amoxicillin, Cephalothrin, Cefotaxime, Ciprofloxacin and Cefoxitin, oxytetracycline and Ceftazydim. However, all the ESBL E. coli isolates from various sources were sensitive to Meropenem (100 %) followed by Gentamicin and Imipenem (80.52 per cent). The results of Multiple Antibiotic Resistance (MAR) index of ESBL and Non-ESBL E. coli indicated that majority of the ESBL E. coli had MAR index between 0.7 to 0.9, whereas majority of the non-ESBL E. coli had MAR 0.4 to 0.5. Correlation between the hygienic condition of the retail outlets, risk factors such as source of water, type of cutting board and butchers hygiene suggested a significant impact on the prevalence of E. coli and ESBL E. coli. Phylogenetic analysis of E. coli revealed that majority of the isolates belonged to group B2 (59.39 %) and D (13.10 %), which indicated that the E. coli isolated were virulent and extra-intestinal strains, whereas only 13.97 per cent could be categorized as commensal.