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Subject: Livestock Product Technology × Author: BHARATH, R. × Start date: 2017 × Type: Thesis ×
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    ThesisItemOpen Access
    UNDERSTANDING THE SHEDDING PATTERN OF ANTIMICROBIAL RESISTANT Escherichia coli IN GOAT MEAT PRODUCTION SYSTEM: A LONGITUDINAL STUDY
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2022) BHARATH, R.; S. WILFRED RUBAN)
    A longitudinal study to understand the shedding pattern of antimicrobial resistant E. coli in goat meat production system was carried in an intensive goat farm in Karnataka. A total of 6 does along with 12 kids were recruited and samples were collected from the host related samples, human and environment at weekly intervals for a period of 6 months (n=690). Antibiotics were used in the farm for only for treatement and enrofloxacin was the most commonly used antibiotic (68-72%), followed by tetracycline and sulfonamide. The overall occurrence of E. coli, tetracycline, fluoroquinolone, Sulfonamide and ESBL resistant E. coli from various samples from goat production system was 81.74, 42.75, 31.25, 48.26 and 16.23 per cent, respectively. Highest occurrence of AMR E. coli was observed in environmental, human and rodent samples. Week wise occurrence revealed an increased in AMR between 7th to 13th week in host related samples and kids had highest (P˃ 0.05) AMR as compared to does. Rodent samples had significantly higher (P˃ 0.05) AMR followed by human toilet swab and soil among the environmental samples throughout the study period. A total of 913 E. coli isolates were screened for presence of genes encoding AMR and it was observed that majority of the isolates carried tetA, qnrB (62.87%), sul2 gene (53.01%), qnrS (30.66%), blaCTX-M (30.34%), blaTEM (27.71%) genes and 12.69 per cent of the isolates were colistin resistant. Occurrence of TRG, FRG and SRG was consistent in host related and environmental samples, however a significantly (P˃ 0.05) higher occurrence of ESBL genes was evident in human, soil and rodent samples. A cyclical pattern in occurrence of AMR genes could be observed throughout with peak occurrence during 4th-13th week and all the genes revealed a decreasing trend at the end of the study. Comparison between does and kids indicated an increase and decrease in AMR genes during the study period indicating the role of antibiotic administration and host immune response. This longitudinal study is the first of its kind in India and the results of this study have provided baseline data on antimicrobial use, dynamics of phenotypic and genotypic resistance to antibiotics in E. coli in intensive goat production system.