Thumbnail Image

Theses (PG)

Browse

20181
Reset filters
Subject: Biochemistry × Author: KAVYA, V. × End date: 2018 × Start date: 2018 × Type: Thesis ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    DEVELOPMENTAL GENE EXPRESSION DURING EARLY FOLLICULOGENESIS IN CAPRINE OVARY
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-09) KAVYA, V.; Dr. V. GIRISH KUMAR
    India occupies first position in terms of goat population and goat milk production. Primordial follicles constituted a store of germ cells in the postnatal ovary and their number varies with species and age. Mammalian ovaries which contain large number of primordial follicles are considered as prime candidates for invitro culture techniques. The present study aim at the isolation of preantral follicles from slaughtered caprine ovaries by using mechanical cum enzymatic method, how to assess the growth rate, viability rate and antrum formation fate of isolated preantral follicle and then expression of some key development genes SPFs, EPFs and LPFs were cultured invitro for 30 , 14 and 7 days in culture medium with growth supplements like glutamine, hypoxanthine and sodium pyruvate, insulin transferring selenium and follicle stimulating hormone respectively and examined for their growth rate, viability and for antrum formation. The results revealed late cultured Preantral follicle has more growth rate, survivability and antrum formation compared to EPFs. Within the growing follicles, some of the genes are upregulated and few genes are downregulated, which was essential for the development of dominant follicle to undergo the ovulation. The mRNA expression of IGF1, EGF, FSHr and FGF was found to be significantly higher in SPF, EPF and LPF respectively by qPCR. We found significantly (P<0.05) higher mRNA expression of FSHr in SPFs while lower expression in LPFs. Similarly, EGF mRNA expression is significantly (P<0.05) higher in LPFS and lower in EPFs. IGF-1 and bFGF mRNA expression significantly higher in LPFs compared to SPFs followed by EPFs. The results indicated that an interaction between EGF and IGF-I and FGF formed an important regulatory mechanism for preantral folliculogenesis.