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Subject: Biochemistry × Author: KALPANA, K. N. × End date: 2021 × Start date: 2021 × Type: Thesis × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    STUDIES ON PROTEIN PROFILE IN TIGERS
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2021-02) KALPANA, K. N.; V. GIRISH KUMAR
    Tiger (Pantheratigris) is an important member of forest ecology belonging to subfamily felinae and they classified as endangered and three subspecies are already extinct out of nine subspecies from the globe. The cause for extinction is postulated to be poaching, a decline in pery base, nutritional deficiencies and/or infectious diseases. Further, the above scenario creates an immense need for conserving the germplasm in tigers. Thus the present study is being undertaken in Royal Bengal tigers to study the suitable microsatellite marker for species conservation as well as to find out a suitable marker in connection with inbreeding. Further Protein profile, GDH, CK activity concerning the age and sex also studied. Upon microsatellite marker analysis a total of 9 alleles were identified across the 4 microsatellite loci, 2 alleles each for Primer 4 (175 and 180 bp), Primer 3 (130 and 160 bp), Primer 2 (500 and 510 bp) and 3 alleles for Primer 1 (100, 120 and 340 bp). Similarly based on PIC values, Primer 4 and Primer 2 showed lower diversity and Primer 3 showed intermediate diversity, whereas Primer 1 showed high diversity. Further, the mean FIS in the present study indicates the probability of inbreeding in the population. Likewise, Shannon's Information index for Primer 1 in the present study indicates the higher genetic diversity in the microsatellite locus in the population. Likewise, serum protein analysis reveals that values in Group-II male tigers were significantly higher when compared to Group-I male tigers, whereas in females, there was no significant difference in both the groups. Similarly, when compared between different sex within a group, a significantly higher level was observed only in Group II male tigers when compared to Group II female tigers. Similarly, the mean albumin values when compared between the two age groups (Group-I and Group-II) within sex as well as when compared between sexes within an age group revealing no significant difference. Likewise, the mean values of α, β1, β2 and γ globulin when compared between the two age groups within sex revealed for no significant differences. Mean serum GDH values in Group I animals of both sex was found to be significantly higher when compared to that in Group II animals. Further, the values of the same when compared between sex within an age group was found to be higher in males when compared to females. Likewise, mean serum CK values revealed for significantly higher value in the Group II male tigers and Group I female tigers when compared to Group I male tigers and Group II female tigers respectively. Further, the mean serum CK values found to be significantly higher in males compared to females in both groups. Keywords: Bengal tiger, microsatellite markers, protein analysis, GDH, CK