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ThesisItem Open Access DIETARY INCORPORATION OF ORGANIC ACID SALT, SODIUM ACETATE ON GUT HISTOLOGY AND IMMUNOMODULATION IN INDIAN MAJOR CARP LABEO ROHITA(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR., 2021) SURYAKIRAN, M.,; GANAP[ATHINAIKOrganic acids in various forms can be used as dietary supplements in aquaculture. This study was conducted to evaluate the use of an acetic acid salt, sodium acetate as a feed additive to enhance growth and health in rohu fish. A total of 120 healthy Labeo rohita fingerlings with an average body weight of 5.0 ± 0.5 g (mean SE) were randomly divided into four equal groups (F0, F1, F2, and F3). F0 was fed a basal diet with no organic acid additive taken as the control group, while F1, F2, and F3 were fed the diets supplemented with 10, 20 and 30 g/kg of sodium acetate salt, respectively. Each group was subdivided in triplicate with 10 fish in each tank. The experimental fish was evaluated after 90 days for growth performance, hematology, immunology, resistance to infection, body composition, gut and liver histology. At the end of the feeding trail, F2 and F3 had significant improvements in final body weight (FBW), weight gain (WG), specific growth rate (SGR) and food conversion ratio (FCR) compared with other groups. The total erythrocyte count, hemoglobin content, platelet count, mean corpuscular hemoglobin and total leukocyte count were significantly increased in F2 and F3 compared to F0 and F1. There were significant differences among the groups in mean corpuscular hemoglobin concentration with F2 as highest. After 90 days. F2 and F1 showed highest superoxide anion activity, lysozyme activity, and total serum protein. The lowest values for immune parameters were recorded in F0. The protein and fat contents of the whole body were the highest in F2 followed by F1 groups. The best protection against challenged Aeromonas hydrophila was in F2 followed by F1 groups. The histological architecture and cellular components of gut showed an improvement in the treatments over the control. The study indicates the efficacy of addition of sodium acetate in enhancing the growth and health in rohu fish Labeo rohita.ThesisItem Open Access EFFECTS OF SODIUM PROPIONATE AS GROWTH STIMULANT AND IMMUNOMODULATOR IN THE DIET OF INDIAN MAJOR CARP LABEO ROHITA(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR., 2021) KANTHA RAJA, P.,; ganapathi naikThis study was conducted to evaluate the use of a propionic acid salt, sodium propionate as a feed additive to enhance growth and health in rohu fish. A total of 120 healthy Labeo rohita fingerlings with an average body weight of 7.5 ± 0.5 g (mean SE) were randomly divided into four equal groups (F0, FI, F2, and F3). F0 was fed a basal diet with no organic acid additive taken as the control group, while FI, F2, and F3 were fed the diets supplemented with 10, 20 and 30 g/kg of sodium propionate salt, respectively. Each group was subdivided in triplicate with 10 fish in each tank. The experimental fish was evaluated after 90 days for growth performance, hematology. Immunology, resistance to infection, body composition, gut and liver histology. At the end of the feeding trail. F2 and F3 had significant improvements in final body weight (FBW), weight gain (WG), specific growth rate (SGR) and food conversion ratio (FCR) compared with other groups. The total erythrocyte count, hemoglobin content, platelet count, mean corpuscular hemoglobin and total leukocyte count were significantly increased in F2 and F3 compared to F0 and F1. There were significant differences among the groups in mean corpuscular hemoglobin concentration with F3 as highest. After 90 days. F2 and F3 showed highest superoxide anion activity, lysozyme activity, and total serum protein. The lowest values for immune parameters were recorded in F0. The protein and fat contents of the whole body were the highest in F3 followed by F2 groups. The best protection against challenged Aeromonas hydrophila was in F3 followed by F2 groups. The histological architecture and cellular components of gut showed an improvement in the treatments over the control. The study indicates the efficacy of addition of sodium propionate in enhancing the growth and health in rohu fish Labeo rohita.ThesisItem Open Access DIETARY INCORPORATION OF ORGANIC ACID SALT, SODIUM ACETATE ON GUT HISTOLOGY AND IMMUNOMODULATION IN INDIAN MAJOR CARP LABEO ROHITA(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR., 2021) SURYAKIRAN, M.,; M.GANAPATHIOrganic acids in various forms can be used as dietary supplements in aquaculture. This study was conducted to evaluate the use of an acetic acid salt, sodium acetate as a feed additive to enhance growth and health in rohu fish. A total of 120 healthy Labeo rohita fingerlings with an average body weight of 5.0 ± 0.5 g (mean SE) were randomly divided into four equal groups (F0, F1, F2, and F3). F0 was fed a basal diet with no organic acid additive taken as the control group, while F1, F2, and F3 were fed the diets supplemented with 10, 20 and 30 g/kg of sodium acetate salt, respectively. Each group was subdivided in triplicate with 10 fish in each tank. The experimental fish was evaluated after 90 days for growth performance, hematology, immunology, resistance to infection, body composition, gut and liver histology. At the end of the feeding trail, F2 and F3 had significant improvements in final body weight (FBW), weight gain (WG), specific growth rate (SGR) and food conversion ratio (FCR) compared with other groups. The total erythrocyte count, hemoglobin content, platelet count, mean corpuscular hemoglobin and total leukocyte count were significantly increased in F2 and F3 compared to F0 and F1. There were significant differences among the groups in mean corpuscular hemoglobin concentration with F2 as highest. After 90 days. F2 and F1 showed highest superoxide anion activity, lysozyme activity, and total serum protein. The lowest values for immune parameters were recorded in F0. The protein and fat contents of the whole body were the highest in F2 followed by F1 groups. The best protection against challenged Aeromonas hydrophila was in F2 followed by F1 groups. The histological architecture and cellular components of gut showed an improvement in the treatments over the control. The study indicates the efficacy of addition of sodium acetate in enhancing the growth and health in rohu fish Labeo rohita.ThesisItem Open Access EFFECTS OF SODIUM PROPIONATE AS GROWTH STIMULANT AND IMMUNOMODULATOR IN THE DIET OF INDIAN MAJOR CARP LABEO ROHITA(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR., 2021) KANTHA RAJA, P; M.GANAPATHI NAIKThis study was conducted to evaluate the use of a propionic acid salt, sodium propionate as a feed additive to enhance growth and health in rohu fish. A total of 120 healthy Labeo rohita fingerlings with an average body weight of 7.5 ± 0.5 g (mean SE) were randomly divided into four equal groups (F0, FI, F2, and F3). F0 was fed a basal diet with no organic acid additive taken as the control group, while FI, F2, and F3 were fed the diets supplemented with 10, 20 and 30 g/kg of sodium propionate salt, respectively. Each group was subdivided in triplicate with 10 fish in each tank. The experimental fish was evaluated after 90 days for growth performance, hematology. Immunology, resistance to infection, body composition, gut and liver histology. At the end of the feeding trail. F2 and F3 had significant improvements in final body weight (FBW), weight gain (WG), specific growth rate (SGR) and food conversion ratio (FCR) compared with other groups. The total erythrocyte count, hemoglobin content, platelet count, mean corpuscular hemoglobin and total leukocyte count were significantly increased in F2 and F3 compared to F0 and F1. There were significant differences among the groups in mean corpuscular hemoglobin concentration with F3 as highest. After 90 days. F2 and F3 showed highest superoxide anion activity, lysozyme activity, and total serum protein. The lowest values for immune parameters were recorded in F0. The protein and fat contents of the whole body were the highest in F3 followed by F2 groups. The best protection against challenged Aeromonas hydrophila was in F3 followed by F2 groups. The histological architecture and cellular components of gut showed an improvement in the treatments over the control. The study indicates the efficacy of addition of sodium propionate in enhancing the growth and health in rohu fish Labeo rohitaThesisItem Open Access INFLUENCE OF SUBSTRATE ON HETEROTROPHIC FOOD PRODUCTION AND GROWTH OF LABEO FIMBRIATUS, BARBODES CARNATICUS AND MACROBRACHIUM ROSENBERGII(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2021) PATKAR ADITI RAJAN; MANJAPPA NIn search of low cost aqua farming, a range of substrate based aquatic system has been developed for both fin fish and shell fish culture which provides shelter and increases the periphyton production as food and thereby increases the production. The present study is aimed to assess the growth and survival of fish (Labeo fimbriatus, Borbodes carnaticus) and shell fish (Macrobrachium rosenbergii) in a substrate based system for a period of six months, where the sugarcane bagasse is used as a substrate for the growth of periphyton. The experiment was divided in three treatments, T1 (only substrate), T2 (substrate + feed) and T0 (control) with triplicates. At the end of the experiment, the growth results showed maximum average weight in T2 (L. fimbriatus 141.17 ± 0.66; B. carnaticus 98.07 ± 1.16 and M. rosenbergii 73.95 ± 0.73) compared with T1 and T0 and there is a significant difference (P<0.05) observed between the treatments, the survival percentage was observed high in T2 (89.81 %) followed by T1 (85.19%) and T0 (83.33 %). The yield (Kg/ha) is more in the T2 (932.65) than T1 (708.56) and T0 (728.89). Periphyton growth has influence the water quality parameters viz., pH (6.73 - 8.17), dissolved oxygen (6.70 - 8.50mg/l). Low concentration of Ammonia (0.068 - 0.140 mg/l) and Nitrite (0.046 - 0.082mg/l) was observed in substrate based ponds. The chlorophyll-a concentration was high in periphyton (0.25 - 3.97μg/cm2) than the plankton (0.58 - 2.64μg/l). The periphyton on substrate increased the protein content of the fish and prawn carcass. The results of the experiment showed that the use of sugarcane bagasse as a substrate not only helped for enhancement of growth, survival and production of the fish and prawn but lowered the levels of toxic NH3-N, NO2-N in water columns. Therefore this system is ecofriendly as limited water exchange is carried in this system. Use of substrate reduces the feed quantity so this technique is farmer’s friendly.