Loading...
Theses (PG)
Browse
14 results
Search Results
ThesisItem Open Access A COMPARATIVE STUDY ON GENETIC POLYMORPHISM OF MYOSTATIN AND FABP3 GENES IN MANDYA AND YALAGA SHEEP(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2021) MEENAKSHI, R. N.; R. NAGARAJA)The PCR-RFLP and SSCP polymorphisms of Myostatin (exon 1 and intron 1) and FABP3 (intron 3) genes were compared between Mandya and Yalaga sheep using the genomic DNA obtained from 50 each of Mandya and Yalaga sheep breeds. The Myostatin (497 bp and 414 bp) and FABP3 (355 bp) gene sequences were amplified by PCR employing published primers. PCR-RFLP analysis of Myostatin gene (497 bp) with DraI restriction enzyme revealed two genotypes, AB and BB with frequencies of 0.34 and 0.66 in Mandya sheep and 0.12 and 0.88 in Yalaga sheep, respectively. The allele frequencies for A and B were 0.17 and 0.83 in Mandya sheep and 0.06 and 0.94 in Yalaga sheep, respectively. PCR-RFLP analysis of FABP3 gene with BanII restriction enzyme revealed two genotypes, AA and AG with frequencies of 0.66 and 0.34 in Mandya sheep and 0.08 and 0.92 in Yalaga sheep, respectively. The gene frequencies for A and G were 0.83 and 0.17 in Mandya and 0.54 and 0.46 in Yalaga sheep, respectively. Multiple sequence alignment of B allele sequences of Myostatin (497 bp) gene and A allele sequences of FABP3 gene in Mandya and Yalaga sheep revealed T>C transition at 64 bp and C>T transition at 174 bp in Yalaga sheep. PCR-SSCP analysis of Myostatin (414 bp) gene revealed two genotypes, AA and AB with frequencies of 0.74 and 0.26 in Mandya sheep and 0.78 and 0.22 in Yalaga sheep, respectively. The gene frequencies for A and B were 0.87 and 0.13 and 0.89 and 0.11 in Mandya and Yalaga sheep, respectively. The alignment of A and B alleles of Myostatin gene in Mandya and Yalaga sheep revealed two SNPs: G>T transversion at position 46 bp and G>T transversion at position 287 bp in Yalaga sheep.ThesisItem Open Access MOLECULAR GENETIC STUDY OF POU1F1 GENE IN MANDYA AND NARI-SUWARNA SHEEP(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2019-08) ABHILASH, H. R.; NAVEEN KUMAR, S.The present study was conducted to determine and compare the polymorphism of POU1F1 gene in Mandya and NARI-Suwarna Sheep. A total of 100 unrelated animals comprising of 50 each of Mandya and NARI-Suwarna sheep were utilized for the present study. Millers high salt method was followed to isolate the genomic DNA from the venous blood. The PCR amplification of exon 3 and exon 4 regions of POU1F1 gene was done by employing published primers, whereas, to amplify exon 6 region of POU1F1 gene primers were designed by using PRIMER 3 PLUS software. The PCR products of sizes 365 bp, 508 bp and 501 bp were resolved on 1.5 per cent agarose gel electrophoresis for exon 3, exon 4 and exon 6 region of POU1F1 gene, respectively. PCR-RFLP analysis of exon 3 and exon 4 regions of POU1F1 gene with AluI and EcoRI restriction enzymes revealed only one allele, A and one genotype AA in both Mandya and NARI-Suwarna sheep indicating monomorphism. The PCR-SSCP analysis of exon 6 region of POU1F1 gene revealed two patterns, P1 and P2. The frequency of P1 and P2 was 98 and 2 per cent, respectively in Mandya sheep and 90 and 10 per cent, respectively in NARISuwarna sheep. The alignment of P1 and P2 pattern sequences of Mandya sheep revealed two SNPs, G to C transversion at 109 bp position and T to A transversion at 112 bp position. Whereas, alignment of P1 and P2 pattern sequences of NARI-Suwarna sheep revealed 3 SNPs, T to G transversion at 218 bp, G to A transition at 225 bp and T to A transversion at 264 bp positions. From the above findings, it may be concluded that polymorphism was established at exon 6 region of POU1F1 gene in both Mandya and NARI-Suwarna sheep by PCR-SSCP analysis. However, present results need to be validated with large sample size.ThesisItem Open Access MOLECULAR GENETIC STUDY OF POU1F1 GENE IN MANDYA AND NARI-SUWARNA SHEEP(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2019-08) ABHILASH, H. R.; NAVEEN KUMAR, S.The present study was conducted to determine and compare the polymorphism of POU1F1 gene in Mandya and NARI-Suwarna Sheep. A total of 100 unrelated animals comprising of 50 each of Mandya and NARI-Suwarna sheep were utilized for the present study. Millers high salt method was followed to isolate the genomic DNA from the venous blood. The PCR amplification of exon 3 and exon 4 regions of POU1F1 gene was done by employing published primers, whereas, to amplify exon 6 region of POU1F1 gene primers were designed by using PRIMER 3 PLUS software. The PCR products of sizes 365 bp, 508 bp and 501 bp were resolved on 1.5 per cent agarose gel electrophoresis for exon 3, exon 4 and exon 6 region of POU1F1 gene, respectively. PCR-RFLP analysis of exon 3 and exon 4 regions of POU1F1 gene with AluI and EcoRI restriction enzymes revealed only one allele, A and one genotype AA in both Mandya and NARI-Suwarna sheep indicating monomorphism. The PCR-SSCP analysis of exon 6 region of POU1F1 gene revealed two patterns, P1 and P2. The frequency of P1 and P2 was 98 and 2 per cent, respectively in Mandya sheep and 90 and 10 per cent, respectively in NARISuwarna sheep. The alignment of P1 and P2 pattern sequences of Mandya sheep revealed two SNPs, G to C transversion at 109 bp position and T to A transversion at 112 bp position. Whereas, alignment of P1 and P2 pattern sequences of NARI-Suwarna sheep revealed 3 SNPs, T to G transversion at 218 bp, G to A transition at 225 bp and T to A transversion at 264 bp positions. From the above findings, it may be concluded that polymorphism was established at exon 6 region of POU1F1 gene in both Mandya and NARI-Suwarna sheep by PCR-SSCP analysis. However, present results need to be validated with large sample size.ThesisItem Open Access MOLECULAR CHARACTERIZATION OF MBL1 GENE AND ITS ASSOCIATION WITH SOMATIC CELL COUNT IN DEONI AND CROSSBRED CATTLE(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2019-09) KHAMGAL PRASHANT RAMCHANDRA; YATHISH, H. M.)The present investigation was undertaken to explore the nucleotide variabilities in coding regions of MBL1 gene using PCR-RFLP and to associate them with somatic cell count (SCC) in 64 Deoni and 50 HF crossbred cattle. In Deoni cattle, only MBL1_E5/StyI analysis revealed AA and AB genotypes, and remaining MBL1_E1/HinfI, MBL1_E2/MslI, MBL1_E3/MmeI and MBL1_E4/BcoDI analysis revealed monomorphism. The frequency of genotypes was varying from 0.17 to 1.0 and that of ‘A’ and ‘B’ alleles was varying from 0.41 to 1.0 in Deoni cattle. In HF Crossbred cattle, MBL1_E2/MslI, analysis revealed AA and AB genotypes, and remaining MBL1_E1/HinfI, MBL1_E3/MmeI, MBL1_E4/BcoDI and MBL1_E5/StyI analysis revealed monomorphism. The frequency of genotypes was varying from 0.28 to 1.00 and that of ‘A’ and ‘B’ alleles was varying from 0.36 to1.00 in HF crossbred cattle. Sequence analysis, when compared to Bos taurus sequence, has revealed three SNPs viz. T244C, G392A and G437A and two SNPs viz. G209A and T244C in ‘A’ allele of MBL1_E2/MslI in Deoni cattle and HF Crossbred cattle, respectively. χ 2 -square analysis revealeThesisItem Open Access GENETIC STUDIES ON LOCAL GOATS IN MANDYA DISTRICT OF KARNATAKA(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2016-05) SIDDALINGA MURTHY, H. K.; (M.R. JAYASHANKAR; K. ANIL KUMAR); C.S. NAGARAJA; H.N. NARASIMAHA MURTHY; K. SATHYANARAYAN; R. BHASKARANA study was undertaken on local goats in Mandya district of Karnataka for various morphological characters, production and reproduction parameters, heritability of body weight at different ages and to develop prediction equations for adult body weight. Characteristic features of the goats were predominantly black colour of coat, eyelid, muzzle and hooves, leafy structured and pendulous ears, straight forehead, absence of beard and wattles and medium sized tail. Among horned goats 76.20 per cent had straight horns with upward backward orientation. Overall least squares means for body weight at three, six, nine, twelve month and adult goats were 9.41±0.12, 15.44±0.09, 19.46±0.09, 21.87±0.09 and 27.20±0.05 kg, respectively. Males were significantly (P≤0.01) heavier than females in all age groups. Single born kids were heavier than twin and triplet born kids. Effects of sex, type of birth and location/ centre on all body measurements were significant. Overall mean age at first conception, age at first kidding, kidding interval and service period in Mandya local goats were 398.94±3.31, 550.07±3.31, 276.00±2.17 and 125.47±0.93 days, respectively, and were significantly affected by year, season and type of birth. Heritability estimates of body weight were 0.36±0.03, 0.29±0.02, 0.27±0.04 and 0.24±0.02 at three, six, nine and twelve months, respectively. Body weight was positively and highly significantly correlated with all the four body measurements viz., height at withers (0.80), body length (0.96), chest girth (0.94) and paunch girth (0.94). Multiple regression analysis indicated that Height at Withers (X1) in combination with Body Length (X2) and Chest Girth (X3) would significantly improve accuracy of predictions.ThesisItem Open Access Evaluation of Second Generation (S1) of Indigenous Chicken Pertaining to two Divisions of Karnataka(Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar, 15-01-15) Mohan Kumar, T. R.; Narasimha Murathy, H.N.; Jayashankar, M.R.; Nagaraja, C.S.; Shri Krishna IsloorA study was undertaken to evaluate the S1 generation of indigenous chicken pertaining to Bengaluru and Mysore divisions of Karnataka, being maintained at AICRP on Poultry Breeding for Meat, Bengaluru centre and to compare with S0 generation for morphological and production traits as per NBAGR proforma. The morphological features recorded revealed 100% normal feather morphology indicating no structural variations in feather such as frizzle, silky and crested. The most prevalent plumage colour observed was brown followed by mixed and black. The most prevalent primary plumage pattern observed was solid, dull, patchy and mottled. The major secondary plumage pattern observed was self-red and self-black colour. Other secondary plumage patterns recorded were self-white, self-blue, mottled, barred and lacing. All the birds evaluated had red ear lobes. Major eye colour observed was brown and grey. Majority of birds had yellow shank. The predominant egg shell colour recorded was brown followed by light brown and creamy. The average egg weight of indigenous chicken ranged from 45.22 g to 46.53 g. The shape index was between 74 to 75. The average values for albumen weight, albumen index, yolk weight, yolk index, Haugh unit score and shell thickness of the eggs ranged from 23 g to 24 g, 0.061 to 0.068, 14 g to 16 g, 0.42 to 0.44, 65 to 70 and 0.35 mm. The present study indicated no significant difference between the two generations with respect to morphological and performance parameters.ThesisItem Open Access Studies on Genetic Polymorphism of Alpha S1 Casein Gene in Nandidurga Goats(Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar, 12-12-14) Umesha Kavali; Jayashankar, M.R.; Nagaraja, R.; Bhaskaran, R.; Girish Kumar, V.A study was conducted with the objective of analyzing the genetic variation at Alpha S1 Casein gene region in Nandidurga non-descriptive goats of Karnataka by using PCR - RFLP technique. Genomic DNA was isolated from blood samples of 85 animals. The genetic diversity of caprine Alpha S1CSN locus was investigated by PCR - RFLP by amplifying part of intron 8, exon 9 and part of intron 9 of the Alpha S1 CSN gene. Restriction endonuclease enzyme XmnI was used to detect the genetic variation of the experimental unit in Alpha S1 CSN gene. Upon PCR - RFLP analysis, two patterns were observed which allowed the identification of two alleles A and B, and two genotypes, AA and BB. Further PCR - RFLP study in 35 Osmanabadi and 50 Bidri goats prevalent in North Karnataka revealed similar observations. Ironically AB type was not observed in any of these goats. The sequence analysis indicated that there was a high homology between the present result and the published caprine Alpha S1CSN gene sequences. There was query coverage to the extent of 100 per cent. This is one of the first studies in non-descript goats. Presence of predominant 'A' allele among these goats provides other selection criteria in addition to traditional selection methods, for increased milk protein.ThesisItem Open Access Study on Genetic Polymorphism of Dazl Gene in Sheep(Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar, 15-11-14) Vageesh Pandith, S.; Nagaraja, R.; Jayashankar, M.R.; Nagaraja, C.S.; Krishnaswamy, A.The present study was carried out with objective of identifying and analyzing the genetic polymorphism in Ovine DAZL gene of native sheep (Mandya sheep), using PCRRFLP technique. A total of sixty five (65) Mandya sheep were randomly selected from two different Mandya sheep flock units viz., Livestock Research and Information Centre (Sheep), KVAFSU, Nagamangala and Department of Instructional livestock Farm complex, Veterinary College, KVAFSU, Bengaluru. From each representative animal, about 10 ml of venous blood was collected in vaccutainer tubes containing 0.5 per cent EDTA. The blood samples were immediately transported to the laboratory at 4 °C and genomic DNA was isolated within 24 hrs. A 655 bp fragment of Ovine DAZL gene sequence spanning part of exon 3, intron 3 and part of exon 4 was amplified by following standard PCR procedure. The PCR amplified sequence of Ovine DAZL gene was confirmed through nucleotide sequencing. Upon RFLP analysis using DdeI restriction enzyme, a polymorphic pattern revealed two alleles viz., Allele-A (348 and 307 bp fragments) and Allele-B (348, 153 and 154 bp fragments). In the studied population of Mandya sheep the allelic frequencies for A and B were 0.62 and 0.37, respectively. The frequencies of AA, AB and BB genotypes were 0.43, 0.38 and 0.18, respectively. The sequence analysis revealed two novel SNPs with transitions viz., C→T and T→G in Intron 3 of Ovine DAZL gene. BLASTn sequence analysis revealed high homology (99 per cent) with that of predicted Ovine DAZL gene (NC_019458) sequence of Ovine chromosome one.ThesisItem Open Access Study on Beta Casein Polymorphism in Malnad Gidda and Crossbred Cattle(Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar, 12-08-14) Navyashree, T.C.; Nagaraja, C.S.; Jayashankar, M.R.; Satyanarayan, K.; Shettar, V.B.The exon 7 region of beta casein gene of Malnad Gidda and crossbred cattle was studied with an objective of analyzing its polymorphism. PCR–RFLP analysis of the amplified region revealed monomorphic pattern in Malnad Gidda and polymorphic pattern in crossbred cattle. Only A2A2 genotype was found in Malnad Gidda cattle. A2 allele was predominant in crossbred cattle with an allelic frequency of 0.79 while A1 allelic frequency was 0.21. The frequencies of A1A1, A1A2 and A2A2 genotypes were 0.13, 0.18 and 0.69 respectively, in crossbred cattle. The Malnad Gidda population was in Hardy Weinberg equilibrium indicating absence of selection pressure while crossbred population was not in equilibrium. The sequence analysis confirmed the difference between A1 and A2 allele due to SNP resulting in substitution of ‘C’ to ‘A’ causing replacement of proline by histidine. The present study confirmed the theory that A2 allele of beta casein is predominant in Bos indicus and crossbred cattle possess both A1A2 heterozygotes and A2A2 homozygotes.
