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ThesisItem Open Access STUDY ON GENETIC POLYMORPHISM OF LEPTIN, BRCA1, C4A AND CXCR1 GENES AND THEIR ASSOCIATION WITH MASTITIS IN HOLSTEIN FRIESIAN CROSSBRED AND DEONI CATTLE(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-07) SHANKARAPPA BHAJANTRI; Dr. M. R. JAYASHANKARA study was conducted to investigate genetic polymorphism of Leptin, BRCA1, C4A and CXCR1 genes and their association with mastitis in HF crossbred and Deoni cows. Milk and blood samples were collected from 152 HF crossbred and 92 Deoni cows. The animals were grouped as mastitis affected and mastitis free mainly based on SCC due to its specificity. Genomic DNA was isolated by high salt method from venous blood samples. Specific regions of Leptin (intron 2), BRCA1 (intron 6), C4A (exon 12) and CXCR1 (exon 8) genes were amplified by PCR employing published primers. The amplified products were resolved on 1.5 per cent agarose gel electrophoresis which yielded 1840, 321, 275 and 469 bp amplicons, respectively. Leptin/ Sau3AI analysis identified three genotypes, AA, AB and AC in HF crossbred and two genotypes, AA and AB in Deoni cows. Polymorphism of BRCA1/ Hha1 analysis showed three genotypes viz., AA, BB and AB in HF crossbred and one genotype, AA in Deoni cows. The PCR-RFLP analysis of the targeted region of 275 bp of bovine C4A gene using Rsa1 detected CC and GC genotypes in both HF crossbred and Deoni cows. A significant association was observed between the C4A genotypes and mastitis in HF crossbred cows, where in majority of animals with CC genotype were mastitis free. CXCR1/ Bsa1 analysis identified two genotypes, AA and AG in both HF crossbred and Deoni cows. A significant association was observed between the CXCR1 genotypes and mastitis in Deoni cows, where in majority of animals with AG genotype were mastitis free.ThesisItem Open Access GENETIC STUDIES ON LOCAL GOATS IN MANDYA DISTRICT OF KARNATAKA(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2016-05) SIDDALINGA MURTHY, H. K.; (M.R. JAYASHANKAR; K. ANIL KUMAR); C.S. NAGARAJA; H.N. NARASIMAHA MURTHY; K. SATHYANARAYAN; R. BHASKARANA study was undertaken on local goats in Mandya district of Karnataka for various morphological characters, production and reproduction parameters, heritability of body weight at different ages and to develop prediction equations for adult body weight. Characteristic features of the goats were predominantly black colour of coat, eyelid, muzzle and hooves, leafy structured and pendulous ears, straight forehead, absence of beard and wattles and medium sized tail. Among horned goats 76.20 per cent had straight horns with upward backward orientation. Overall least squares means for body weight at three, six, nine, twelve month and adult goats were 9.41±0.12, 15.44±0.09, 19.46±0.09, 21.87±0.09 and 27.20±0.05 kg, respectively. Males were significantly (P≤0.01) heavier than females in all age groups. Single born kids were heavier than twin and triplet born kids. Effects of sex, type of birth and location/ centre on all body measurements were significant. Overall mean age at first conception, age at first kidding, kidding interval and service period in Mandya local goats were 398.94±3.31, 550.07±3.31, 276.00±2.17 and 125.47±0.93 days, respectively, and were significantly affected by year, season and type of birth. Heritability estimates of body weight were 0.36±0.03, 0.29±0.02, 0.27±0.04 and 0.24±0.02 at three, six, nine and twelve months, respectively. Body weight was positively and highly significantly correlated with all the four body measurements viz., height at withers (0.80), body length (0.96), chest girth (0.94) and paunch girth (0.94). Multiple regression analysis indicated that Height at Withers (X1) in combination with Body Length (X2) and Chest Girth (X3) would significantly improve accuracy of predictions.ThesisItem Open Access A COMPARATIVE STUDY ON MTNR-1A GENE POLYMORPHISM BETWEEN MANDYA AND NARI-SUWARNA SHEEP(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2016) VIBHA, C. RIn India, the livestock sector plays an important role in day-to-day sustenance of rural population and contributes a major share towards agricultural economy.ThesisItem Open Access COMPARATIVE STUDY OF GROWTH HORMONE GENE POLYMORPHISM BETWEEN MALNAD GIDDA AND ONGOLE CATTLE(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2009) S.VINOTH KUMARLivestock is one of the natural resources well distributed among different income groups in rural IndiaThesisItem Open Access MOLECULAR CHARACTERIZATION OF MUDHOL BREED OF DOG(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2017) SOWMYASHREE, B. LIndia is a rich repository of livestock and poultry having the population of 190.90 million cattle, 108.70 million buffalo, 65.06 million sheep, 135.17 million goat and 729.2 million poultry (Anon., 2012)ThesisItem Open Access ASSOCIATION BETWEEN DAZL GENE SINGLE NUCLEOTIDE POLYMORPHISM AND FERTILITY TRAITS IN BREEDING RAMS(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2015) SIDDARAJU, NAmong the small ruminants, sheep constitute an important livestock species in the rural agricultural economy of IndiaThesisItem Open Access COMPARATIVE MOLECULAR GENETIC ANALYSIS OF MILK PROTEIN (KAPPA CASEIN) GENE IN LOCAL AND EXOTIC CATTLE(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2009) SHIVASHANKERGenetic variations of kappa casein gene and their association with milk quality traits were studied in ten Jersey, 25 Hallikar and 25 Malnad Gidda cattle by adopting PCR-RFLP and DNA sequence analysisThesisItem Open Access COMPARATIVE GENETIC EVALUATION OF MILK QUALITY TRAITS IN MALNAD GIDDA AND CROSSBRED CATTLE(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2015) SHILPASHREE, K. SIndia has vast animal genetic resources with wide variety of indigenous farm animals including cattleThesisItem Open Access MORPHOLOGICAL CHARACTERIZATION OF MOULI SHEEP(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2014) SHASHIKANT M. SINDAGIIndia is a rich source of diverse ovine germplasm having a total of 42 sheep breeds with 74 million sheep that accounts for 6.8% of world sheep population (Anonymous, 2010) and ranks third in the world.
