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20181
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Subject: Animal Genetics and Breeding × End date: 2018 × Start date: 2018 × Type: Thesis ×
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    ThesisItemOpen Access
    STUDY ON GENETIC POLYMORPHISM OF LEPTIN, BRCA1, C4A AND CXCR1 GENES AND THEIR ASSOCIATION WITH MASTITIS IN HOLSTEIN FRIESIAN CROSSBRED AND DEONI CATTLE
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-07) SHANKARAPPA BHAJANTRI; Dr. M. R. JAYASHANKAR
    A study was conducted to investigate genetic polymorphism of Leptin, BRCA1, C4A and CXCR1 genes and their association with mastitis in HF crossbred and Deoni cows. Milk and blood samples were collected from 152 HF crossbred and 92 Deoni cows. The animals were grouped as mastitis affected and mastitis free mainly based on SCC due to its specificity. Genomic DNA was isolated by high salt method from venous blood samples. Specific regions of Leptin (intron 2), BRCA1 (intron 6), C4A (exon 12) and CXCR1 (exon 8) genes were amplified by PCR employing published primers. The amplified products were resolved on 1.5 per cent agarose gel electrophoresis which yielded 1840, 321, 275 and 469 bp amplicons, respectively. Leptin/ Sau3AI analysis identified three genotypes, AA, AB and AC in HF crossbred and two genotypes, AA and AB in Deoni cows. Polymorphism of BRCA1/ Hha1 analysis showed three genotypes viz., AA, BB and AB in HF crossbred and one genotype, AA in Deoni cows. The PCR-RFLP analysis of the targeted region of 275 bp of bovine C4A gene using Rsa1 detected CC and GC genotypes in both HF crossbred and Deoni cows. A significant association was observed between the C4A genotypes and mastitis in HF crossbred cows, where in majority of animals with CC genotype were mastitis free. CXCR1/ Bsa1 analysis identified two genotypes, AA and AG in both HF crossbred and Deoni cows. A significant association was observed between the CXCR1 genotypes and mastitis in Deoni cows, where in majority of animals with AG genotype were mastitis free.