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20191
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Subject: Animal Genetics and Breeding × Author: KHAMGAL PRASHANT RAMCHANDRA × End date: 2021 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    MOLECULAR CHARACTERIZATION OF MBL1 GENE AND ITS ASSOCIATION WITH SOMATIC CELL COUNT IN DEONI AND CROSSBRED CATTLE
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2019-09) KHAMGAL PRASHANT RAMCHANDRA; YATHISH, H. M.)
    The present investigation was undertaken to explore the nucleotide variabilities in coding regions of MBL1 gene using PCR-RFLP and to associate them with somatic cell count (SCC) in 64 Deoni and 50 HF crossbred cattle. In Deoni cattle, only MBL1_E5/StyI analysis revealed AA and AB genotypes, and remaining MBL1_E1/HinfI, MBL1_E2/MslI, MBL1_E3/MmeI and MBL1_E4/BcoDI analysis revealed monomorphism. The frequency of genotypes was varying from 0.17 to 1.0 and that of ‘A’ and ‘B’ alleles was varying from 0.41 to 1.0 in Deoni cattle. In HF Crossbred cattle, MBL1_E2/MslI, analysis revealed AA and AB genotypes, and remaining MBL1_E1/HinfI, MBL1_E3/MmeI, MBL1_E4/BcoDI and MBL1_E5/StyI analysis revealed monomorphism. The frequency of genotypes was varying from 0.28 to 1.00 and that of ‘A’ and ‘B’ alleles was varying from 0.36 to1.00 in HF crossbred cattle. Sequence analysis, when compared to Bos taurus sequence, has revealed three SNPs viz. T244C, G392A and G437A and two SNPs viz. G209A and T244C in ‘A’ allele of MBL1_E2/MslI in Deoni cattle and HF Crossbred cattle, respectively. χ 2 -square analysis reveale