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Subject: Animal Genetics and Breeding × Author: Arshan Shaheed, A. × End date: 2021 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    Studies on Genetic Polymorphism of Beta-Lactoglobulin Gene in Nandidurg Goat
    (Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar, 15-09-13) Arshan Shaheed, A.; Nagaraja, C.S.; Jayashankar, M.R.; Girish Kumar, V; Veere Gowda, B.M.
    A study was conducted with the objective of analyzing the genetic variation at the β-lactoglobulin region of Nandidurg non-descriptive breed of goat by using PCR- RFLP technique. Genomic DNA was isolated from blood samples of 85 animals. The genetic diversity of caprine β-lactoglobulin locus was investigated by PCR- RFLP. A 426 bp fragment of exon 7 to the 3‟ flanking region of the β-lactoglobulin gene was amplified. The size of the amplified product was same in all the animals studied indicating conservation of DNA at this locus. A restriction endonuclease SacII was used to detect the genetic variation of the experimental unit in the β-lactoglobulin gene. β-LG/SacII polymorphism was observed as three patterns which allowed the identification of two alleles viz., A and B. The genotypic frequencies of AA, AB and BB were 0.35, 0.54 and 0.11 respectively. The allelic frequencies for A and B alleles were 0.62 and 0.38 respectively, among them allele A was predominant in studied population. The population studied was in Hardy-Weinberg equilibrium. The sequence analysis indicated that there was a high homology between the present result and the published caprine β-lactoglobulin sequences. There was query coverage to the extent of 100 per cent. The present study revealed the genetic variability at β-lactoglobulin gene locus in Nandidurg goat. Further study is suggested to understand the association of this gene with milk production traits.