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Subject: Animal Genetics and Breeding × Author: AJEET SINGH × Start date: 2020 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    COMPARATIVE GENETIC CHARACTERIZATION OF PROLACTIN GENE BETWEEN GIRIRAJA AND INDIGENOUS CHICKEN
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2022) AJEET SINGH; NAVEEN KUMAR, S.
    The present study was undertaken to determine and compare the genetic variations in prolactin gene among Giriraja and Indigenous chicken using PCR-RFLP technique. Blood samples were collected from 80 each of Giriraja and Indigenous chicken from Department of Poultry Science, Veterinary College, Bengaluru. The genomic DNA was isolated by following the conventional Phenol: Chloroform: Iso-amyl alcohol (25:24:1) method as described by Khosravinia et al. (2007). A 130/ 154 bp and 439 bp sequences of promoter regions 1 (24 bp indel at -358) and 2 (C-2402T and C-2161G) of prolactin gene were successfully amplified employing published primers. At promoter region 1 (24 bp indel at -358) of prolactin gene, the proportions of DD and ID genotypes were higher in Giriraja (0.45) and Indigenous (0.44) chicken, respectively. However, the frequency of D allele was higher in both the studied populations. The restriction enzymes, AluI and Csp6I were utilized for genotyping polymorphisms of C -2402T and C -2161G, respectively. At C -2402T, TT and CT genotypes were predominant in Giriraja and Indigenous chicken with frequencies of 0.61 and 0.56, respectively. The frequency of T allele was higher in both the studied populations. Whereas at C-2161G, GG and CG genotypes were predominant in Giriraja (0.49) and Indigenous (0.65) chicken, respectively. In both the studied populations the frequency of G allele was higher. Further, three SNPs viz., C>T transition at 161 bp, T>C transition at 371 bp and C>G transversion at 402 bp were detected at promoter region 2 (C-2402T and C-2161G) in T/ G allele compared to C allele.