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Subject: Biochemistry ×

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Now showing 1 - 9 of 23
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    ThesisItemOpen Access
    Genetic Diversity for Oil Content and Fatty Acid Profiling In Cotton Genotypes Across the Species
    (University of Agricultural Science, Dharwad, 2015-09) Dolas, Chetan Sahebrao; Vamadevaiah, H.M.
    Cotton seed is one of the important by produvts of cotton, the use of which has not been exploited to the fullest extent. The seed collected after ginning is used for extraction of oil. The keeping quality of cotton seed oil is comparable to groundnut and safflower oil and its nutritional value is around 9 k cal/g. The average digestibility of cotton seed oil is is 97 per cent and could be compared with that of soybean, safflower and sunflower oils. The current work aims to study oil content, fatty acid profiling, ginning outturn, seed index and their correlation with oil content. The oil content in 123 cotton genotypes belonging to all four species was analyzed in Oxford 4000 NMR analyzer .Simultaneously; seed index and ginning out turn were estimated in these genotypes and were correlated with oil content. Fatty acid profiling og eight genotypes including all four species of cotton was carried out. The seeds were dehulled and kernel crushed to get fine powder and from this the oil was extracted with hexane in Soxhlet apparatus. Extracted oilwas methyl esterified and qualitative and quantitative analysis of oil was carriedout in Shimadzu Chromatopac GC-MS QP2010S.The major chemical components present in oil samples were palmitic acid, stearic acid, oleic acid and linoleic acid. Among the eight genotypes BCS-23-18-7(G. barbadense) had the highest amount of oleic acid (24.36%). Here the activity of oleate 12 desaturase enzyme may be less and hence the oleic and stearic acid have accumulated and simultaneously linoleic acid has drastically decrased to 44.07 percent.
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    ThesisItemOpen Access
    Provenance Variation in Growth, Seed Morphometric Traits, Biochemical Characterization of Seed Oil of Calophyllum Inophyllum Grown in Uttar Kannada District
    (University of Agricultural Science, Dharwad, 2015-07) Shamala K.V.; Ramana, P.
    Provenance influence on growth, productivity, seed quality, oil content, fatty acid profile, seed cake quality and biodiesel production of Calophyllum inophyllum were conducted in Uttara Kannada district (Agro-climatic Zone-10) of Karnataka during 2014-2015. The study was undertaken in the already existing trees in natural forest at five different locations with varying altitudes (2.7-26.6 m) and rainfall (3209-4145 mm). There was a significant effect of locations on tree growth, productivity and soil chemical properties. Seed parameters were significantly influenced by different locations. Seed oil yield varied from 36.07 to 48.87 per cent and was greenish yellow in colour with disagreeable odour. Four to six fatty acid components were detected in the oil samples of C. inophyllum by GC-MS analysis of which four fatty acids are the major components viz., Palmitic acid ranges from 17.24-23.39 per cent, Stearic acid ranges from 9.88-15.90 per cent, Oleic acid ranges from 24.87-62.73 per cent and Linoleic acid ranges from 21.46-57.23 per cent. Two stage transesterification of C. inophyllum oil yielded biodiesel with 67.07 per cent. Biodiesel having Kinematic viscosity of 1.638 cSt (at 40 °C), Density as 889 (kg/m3 at 30 °C), Acid value of 0.14 (mg KOH/g), Specific gravity of 0.889 (at 30 °C), Saponification value as 147.56, Iodine value of 90.12, flash point of 140 ºC and cetane value of 59.35. The biodiesel parameters fit into the ASTM D6751 standards. Macro nutrients and proximate composition of de-oiled cake differed significantly with respect to locations and sites. C. inophyllum has the potential to capture medicinal and biofuel sector, due to its high oil content and oil quality and biodiesel production potential.
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    ThesisItemOpen Access
    HISTOLOGY AND HISTOCHEMISTRY OF THE ENDOCRINE ORGANS OF THE INDIAN DONKEY (Equus as in us)
    (University of Agricultural Science, Dharwad, 2001) Pawar, Ashok; Ramakrishna, V
    "The present study on the histology and histochemistry of the endocrine organs viz. pineal , Pituitary , thyroid , parathyroid and adrenal gland in the young and adult Indian donkey to know the cytoarchitecture and histochemical characterstics of the cells with their location and functional status. The gross morphological studies revealed the location and position of various endocrine organs in the Indian donkey. Histologically, the pineal gland consisted of pinealocytes, me lanocytes and glial cells in the parenchyma. Th e presence of two types of pinealoc ytes indicate the functional status of the gland. The pituitary gland consisted of a d e n oh yp op h y sis and neurohypophys is. The a d e n o h yp o p h y s is consisted of acidophils, basophils and ch romo p h o be s . Neu ro hyp o ph y s is consisted of pituicytes and neuroglial cells. Herring bodies were noticed in the n ear blood vessels. Thyroid gland mainly consisted of small, me d ium and large follicle Vi/ith blood vessels and parafollicular cells. The presence of small follicles were more in n umb e r and lineu by cuboidal epithelium in young donkey. Large follicles lined by tlat epithelium with loss or no colloid indicatGd ttu? aclivily of the gland. Parathyroid gland consisted, oxyphil,two types pf cliie f cells and parafollicular cells. Th e adrenal gland was consisted of outer cortex, middle g a nglionic and medulla. Epinephrine and norepinephrine cells were noticed in the niedulla. Histochemically. Histochemically the pinealocytes of the pineal, basophils and neurosecretory substances (Herring bodies) o f pituitary, colloid o f tlie thyroid, chief cells and oxyphil cells of parathyroid and zona arcuata and zona reticularis of adrenal gland showed PAS positive reaction. Pinealocytes of pineal, basophils of pituitary, follicular epithelium and colloid o f follicles, chief cells o f parathyroid and ganglionic cells o f the adrenal gland were alcian blue (pH 2.5) positive. No significant difference in the histology and histochemistry between young and adult Indian donkey endocrine glands except pineal and thyroid gland."
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    ThesisItemOpen Access
    POPULATION GENETICS AND GENETIC DIVERSITY STUDIES IN Santalum album L. USING BIOCHEMICAL MARKERS
    (University of Agricultural Science, Dharwad, 1998) Venu, R C; Sudharshana, L
    ABSTRACT NOT AVAILABLE
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    ThesisItemOpen Access
    STUDIES ON BIOCHEMICAL BASIS OF BLAST DISEASE RESISTANCE IN RICE (Oryza sativa L.) SEEDLINGS
    (University of Agricultural Science, Dharwad, 1998) Ashoka, M A; Thimmaiah, S K
    ABSTRACT NOT AVAILABLE
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    ThesisItemOpen Access
    CHARACTERIZATION OF RHIZOBIAL SURFACE COMPONENTS INVOLVED IN Rhizobiam-Cicer arietinum L. SYMBIOSIS
    (University of Agricultural Science, Dharwad, 1998) Joshi, Raghavendra; Podder, Uma S
    ABSTRACT NOT AVAILABLE
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    ThesisItemOpen Access
    PRODUCTION, PURIFICATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO ABSCISIC ACID : ITS APPLICATION IN STRESS PHYSIOLOGY OF PLANTS
    (University of Agricultural Science, Dharwad, 1996) Sreesaila, N; Sudharshana, L
    ABSTRACT NOT AVAILABLE
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    ThesisItemOpen Access
    PHENOLIC METABOLISM IN BLAST INFECTED FINGER MILLET (Eleusine coracana Gaertn ) SEEDLINGS
    (University of Agricultural Science, Dharwad, 1996) Sunil, K V; John, Elizabeth
    ABSTRACT NOT AVAILABLE
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    ThesisItemOpen Access
    STUDIES ON PHOSPHATASES OF BLAST INFECTED FINGER MILLET (Eleusine coracana Gaertn.) SEEDLINGS
    (University of Agricultural Science, Dharwad, 1996) Mohan, Nadiga; John, Elizabeth
    ABSTRACT NOT AVAILABLE