Genetic Diversity for Oil Content and Fatty Acid Profiling In Cotton Genotypes Across the Species
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Date
2015-09
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University of Agricultural Science, Dharwad
Abstract
Cotton seed is one of the important by produvts of cotton, the use of which has not been exploited to the fullest extent. The seed collected after ginning is used for extraction of oil. The keeping quality of cotton seed oil is comparable to groundnut and safflower oil and its nutritional value is around 9 k cal/g. The average digestibility of cotton seed oil is is 97 per cent and could be compared with that of soybean, safflower and sunflower oils. The current work aims to study oil content, fatty acid profiling, ginning outturn, seed index and their correlation with oil content. The oil content in 123 cotton genotypes belonging to all four species was analyzed in Oxford 4000 NMR analyzer .Simultaneously; seed index and ginning out turn were estimated in these genotypes and were correlated with oil content. Fatty acid profiling og eight genotypes including all four species of cotton was carried out. The seeds were dehulled and kernel crushed to get fine powder and from this the oil was extracted with hexane in Soxhlet apparatus. Extracted oilwas methyl esterified and qualitative and quantitative analysis of oil was carriedout in Shimadzu Chromatopac GC-MS QP2010S.The major chemical components present in oil samples were palmitic acid, stearic acid, oleic acid and linoleic acid. Among the eight genotypes BCS-23-18-7(G. barbadense) had the highest amount of oleic acid (24.36%). Here the activity of oleate 12 desaturase enzyme may be less and hence the oleic and stearic acid have accumulated and simultaneously linoleic acid has drastically decrased to 44.07 percent.
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