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Author: Lyngdoh, Nicolee × End date: 2003 × Start date: 2003 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF TEAK (Tectona grandis Linn. f.) CLONES OF KARNATAKA
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, DHARWAD, 2003) Lyngdoh, Nicolee; Vasudeva, R
    "Fifty teak {Tectona grandis L. f.) clones of Karnataka. maintained at Karka clonal bank and at Alur clonal seed orchard near Dandeli, were characterized at morphological and molecular levels apart from assessing their resistance levels to two important pests viz., trunk borer {Alcterogystia cadambe) and defoliatorskeletonizer complex {i-iyblea purea & Eutectona maciieralis). Using a set of seven simple leaf descriptors {viz. leaf petiole, leaf texture, leaf shape, leaf tip, leaf base, adaxial leaf pubescence and leaf colour) a key was developed to delineate teak clones that could be effectively adopted at the field level. Perhaps for the first time, 30 teak clones were fingerprinted with 20 random RAPD primers. Six clones {viz., MyHV2, MyHV3, MyHV7, MySa1, MySa2 and MyHaK2) possessed unique bands enabling them to be identified without ambiguity and further, thirteen pairs of clones shared a unique band. These informative primers show a great potentiality in genotyping unknown clones, maintaining clonal fidelity and in clonal registration. Moderately high level of genetic diversity was found among the 30 teak clones with an information index of 0.373 ± 0.243. Clones of central provenance of Karnataka were more diverse (0.352) than those from southern (0.304) and northern provenance (0.317) as revealed by Shannon Information Index. Significant inter-clonal differences were identified for the susceptibility levels to trunk borer and for defoliators. Eleven of the fifty clones were tolerant to defoliator attack with MyMK3 (24.33% leaf defoliation) and MyHV3 (37.28%) clones showing least attack. Clone MyHuTI showed maximum tolerance to trunk borer (0.44 holes per tree) among the 12 tolerant clones. However, levels of tolerance to these two pests were independent. Association analysis showed that clones with higher pubescence and dark green colour were more often susceptible to defoliators' attack. Clones from southern origin performed better in terms of volume growth after twenty years (0.43 m^) than those of the central (0.41 m^) and northern (0.38 m""^) provenance of the state."
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    ThesisItemOpen Access
    EFFECT OF VAM FUNGI AND VERMICULTURE ON GROWTH, YIELD AND STRESS TOLERANCE OF PAPAYA
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, DHARWAD, 2003) Lyngdoh, Nicolee; Vasudeva, R
    "Fifty teak {Tectona grandis L. f.) clones of Karnataka. maintained at Karka clonal bank and at Alur clonal seed orchard near Dandeli, were characterized at morphological and molecular levels apart from assessing their resistance levels to two important pests viz., trunk borer {Alcterogystia cadambe) and defoliatorskeletonizer complex {i-iyblea purea & Eutectona maciieralis). Using a set of seven simple leaf descriptors {viz. leaf petiole, leaf texture, leaf shape, leaf tip, leaf base, adaxial leaf pubescence and leaf colour) a key was developed to delineate teak clones that could be effectively adopted at the field level. Perhaps for the first time, 30 teak clones were fingerprinted with 20 random RAPD primers. Six clones {viz., MyHV2, MyHV3, MyHV7, MySa1, MySa2 and MyHaK2) possessed unique bands enabling them to be identified without ambiguity and further, thirteen pairs of clones shared a unique band. These informative primers show a great potentiality in genotyping unknown clones, maintaining clonal fidelity and in clonal registration. Moderately high level of genetic diversity was found among the 30 teak clones with an information index of 0.373 ± 0.243. Clones of central provenance of Karnataka were more diverse (0.352) than those from southern (0.304) and northern provenance (0.317) as revealed by Shannon Information Index. Significant inter-clonal differences were identified for the susceptibility levels to trunk borer and for defoliators. Eleven of the fifty clones were tolerant to defoliator attack with MyMK3 (24.33% leaf defoliation) and MyHV3 (37.28%) clones showing least attack. Clone MyHuTI showed maximum tolerance to trunk borer (0.44 holes per tree) among the 12 tolerant clones. However, levels of tolerance to these two pests were independent. Association analysis showed that clones with higher pubescence and dark green colour were more often susceptible to defoliators' attack. Clones from southern origin performed better in terms of volume growth after twenty years (0.43 m^) than those of the central (0.41 m^) and northern (0.38 m""^) provenance of the state."