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Open Access
Electroencephalographic and electrocardiographic studies on propofol anaesthesia in sheep
(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2011-06) Raj Kumari; Das, Arup Kumar
In this study electroencephalographic and electrocardiographic changes during surgical interventions {viz., placement of central venous catheters (Group-B), docking/ tail amputation (Group-C), bilateral orchiectomy (Group-D) and no surgical stimuli as control (Group-A)} with propofol anaesthesia were assessed in twenty-four (n=24) apparently healthy male sheep. For surgical operations each sheep of this study was premedicated with glycopyrrolate (@ 0.02 mg/kg body weight, IM) and, five minutes after, anaesthesia was induced with propofol (@ 4.00 mg/kg body weight, IV). The parameters in this study included clinical, cardiopulmonary, haematobiochemical and electrocardiographic parameters. Similarly, qualitative electroencephalographic evaluation was done through spectral analysis to generate total power, SEF-90, MF and PPF over 0-30 frequency band width while relative power of different bandwidths viz., , , and was assessed. Rectal temperature and respiration rate has not shown any significant variability in any of the groups during the entire course of experiment. Haemoglobin concentration reduced and this extent was higher in control group than central venous catheterization, docking and castration groups. PCV reduced in all the groups. TEC depicted reduction in control group; however, this fall was not apparent in other groups. Blood glucose decreased initially followed by an increase in three groups after propofol induction while the castration group exhibited a non-significant increase from very beginning. Total serum protein declined in all the groups. A sudden increase in serum triglyceride (TG) was observed at one minute post-propofol induction in all the groups, which later on declined. Propofol has not been able to induce any significant changes in P-wave, QRS wave and T-wave amplitude as exhibited in control group. However, P-wave amplitude increased significantly in animals performed with central venous catheterization. The T-wave amplitude increased nonsignificantly in CVC group while decrease in docking and castration groups. P-wave and T-wave duration has not exhibited any significant difference throughout the course of experiment. However, QRS wave duration decreased in control group and no significant changes were observed in surgical groups. PR segment length reduced in castration group only and none of the other groups exhibited any significant changes. ST segment length exhibited significant increase in control group, non-significant increase in CVC while exhibiting significant fall in castration and docking groups. RR interval exhibited reduction in all the four groups, non-significantly in control while significantly in the three surgical groups. Heart Rate increased in all the four groups. EEG analysis showed an increase in total power, median frequency, theta frequency, peak power in all group, and after a transient increase reduction in spectral edge frequency and alpha frequency. However delta frequency showed an increase after a transient decrease and a marked decrease in beta frequency after propofol injection. It was concluded that propofol to some extent possess analgesic effect and may be useful in husbandry practices/ day case surgery. However, evaluation of analgesic effects of propofol needs further investigation.
Open Access
Effect of manual and chemical methods of weed management on potato (Solanum tuberosum L.)
(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2016-06) Lavlesh; Manoj Raghav
The present investigation was undertaken to evaluate the “Effect of manual and chemical methods of weed management on potato (Solanum tuberosum L.)” at Vegetable Research Centre of G.B. Pant University of Agriculture and Technology, Pantnagar (Uttarakhand) during rabi season of 2014-2015. The experiment was laid out in Randomized Block Design consisting of seven treatments replicated thrice. During the study, observations on per cent emergence, plant height, number of haulms per hill, fresh and dry weight of weeds, number of monocot, dicot and total number of weeds at 30, 45, 60 days after planting (DAP) and at de-haulming stage, fresh and dry weight of plants at de-haulming stage were recorded. In addition, grade wise number and weight of tubers per plot and per hectare, total number of tubers and total tuber yield per hectare, marketable yield per hectare, harvest index, dry matter content, protein content and specific gravity of tubers, nitrogen, phosphorus and potash uptake by haulm, tuber, plant and weeds were also recorded. Besides this, to assess the profitability of treatments, cost of cultivation, net profit and benefit: cost ratio were also worked out. The findings of present investigation revealed that performance of potato crop was significantly influenced by different weed control treatments. Among all treatments, herbicide metribuzin @ 0.75 kg a.i./ha pre emergence (T6) was found best with respect to overall plant growth, yield and quality parameters with a total yield (305.43 q/ha), net return (`147497.36) and benefit: cost ratio (1.93). Based on overall performance, it could be concluded that under prevalent climatic conditions of Uttarakhand tarai region, herbicide metribuzin @ 0.75 kg a.i./ha pre emergence is the best in terms of higher and economic yield of potato. Hence, recommended for commercial cultivation, provided all other scientific management practices are followed.
Open Access
Efficacy of blossom thinners on plant growth, fruit retention and quality of plum (Prunus salicina Lindle) cv Kala Amritsari
(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-01) Bhatt, Siddharth Shankar; Dimri, D.C.
The present investigation was carried out at Horticulture Research Centre, Patharchatta, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, District-Udham Singh Nagar, Uttarakhand in the year January- July, 2015 and 2016. The experiment was conducted on 8 years old healthy fruit bearing trees of plum cultivar ‘Kala Amritsari’ having uniform size and vigor. Investigation was laid out in Randomized Block Design (RBD) with three replications and 16 treatments namely; GA3 (25 ppm), GA3 (50 ppm), GA3 (75 ppm), NAA (25 ppm), NAA (50 ppm), NAA (75 ppm), BA (25 ppm), BA (50 ppm), BA (75 ppm), ethephon (100 ppm), ethephon (150 ppm), ethephon (200 ppm), ATS (1.0%), ATS (1.5%), ATS (2.0%)and control (water spray).Observations were recorded on plant growth, flowering, fruit set, fruit physico-chemical and plant physiological parameters. Findings of the present investigation revealed that ATS @ 1.5% recorded maximum extension of shoot growth (32.61 cm), highest number of leaves (34.25) and maximum size of leaves (12.36 cm2) while, highest number of damaged leaves was observed under ATS @ 2.0 % (8.30). In respect to flowering and fruit set parameters the treatment control recorded highest bloom density (50.66%), number of flowers retained (43.36) and number of flower per cluster (8.03). Ethephon @ 150 ppm (80.73 days) however, took least number of days to maturity. The highest initial fruit set (43.70%) though was calculated under the control treatment but maximum fruit retention (34.39%) was counted for ATS @ 1.5% with least fruit drop (65.61%) resulting in highest yield per tree (34.14 kg). Application of the treatment ATS @ 1.5% was found superior among all blossom thinners in enhancing maximum fruit length (4.14 cm), highest fruit width (3.06 cm), maximum fruit weight (21.53 g) and highest fruit volume (22.10 ml). The highest pulp weight (21.35 g) and least stone weight (0.84 g) was also measured under ATS @ 1.5% which as a result calculated highest pulp: stone ratio (27.94). Among different chemical blossom thinners sprayed maximum total soluble solids (12.64), least titrable acidity (0.58), highest ascorbic acid (9.11 mg), maximum total sugar (6.29%) and highest total carotenoides content (10.87 mg) was estimated when ATS @ 1.5% was applied at post bloom stage. Plant physiological parameters was also affected with ATS @ 1.5% which calculated least Chlorophyll ‘a’ content (1.54 mg) and Chlorophyll ‘b’ content (0.88 mg). Based on the present investigation it can be concluded that ATS @ 1.5% was found to be most effective blossom thinner in enhancing plant growth, yield and physico-chemical attributes in plum cv. ‘Kala Amritsari’ under tarai region of Uttrakhand.
Open Access
Seasonal chemical diversity of Acorus calamus L. accessions from different altitudes of Uttarakhand Himalayas: Pharmacological screening and analysis of genetic variability
(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-08) Archana; Om Prakash
In present study the elemental analysis, preliminary phytochemical screening, seasonal and altitudinal chemical composition of essential oils by GC-MS, isolation of compounds by column chromatography, biological activities like antioxidant assay and pharmacological potential of rhizome essential oils/major components (α and β asarone) from A calamus and genetic variability among three accessions were evaluated. The yields of essential oils in different seasons varied from 0.11 to 6.8 % w/v. The elemental analysis studied with the help of atomic absorption spectrometry (AAS), exhibited the presence of N, P, K, Ca, Mg, Ca, Mn, Fe, Cu, Cr, Pb, Ni, Zn. The presence of various secondary metabolites like carbohydrates, proteins, glycosides, flavonoids etc. were observed in all the accessions. GC-MS analysis essential oils, methanolic extracts of leaves and rhizomes of Acorus calamus exhibited various phenylpropanoids, monoterpenoids and sesquiterpenoids. The major compounds identified were trans methyl isoeugenol, Z isoelimicin, α asarone, β asarone and shyobunone. The isolation of the compound from rhizome methanolic extract by column chromatography and characterized by mass spectrometry and NMR proofs the presence of β asarone. The antioxidant activity of essential oils and methanolic extracts showed good to moderate antioxidant potential. The total phenols, flavonols and orthodihydric phenols in all the extracts were ranged from 11.11 to 133.33. 31.70mg/g gallic acid equivalent, 72.64mg/g catechin equivalent and 67.87mg/g respectively. The combination of isomers α+β-asarone at higher dose level of 100 mg/kg b.wt. exhibited significant antipyretic activity (75.10%), analgesic activity(48.87%) and anti-inflammatory activity (26.16%) compared to the standard drugs paracetamol (65.14%), ibuprofen (48.39%) and aspirin (20.98%) respectively. The essential oils and α, β asarones individually also exhibited good pharmacological activities with minimum at lower dose (50mg/kg b.wt.) and maximum at higher dose (100mg/kg b.wt.). The oils and isomers α and β asarones individually and in combination exhibited significant hepatoprotective activity in a dose dependent manner against CCl4 induced hepatotoxicity using silymarin as standard drug. The pharmacological activities associated with the combination of α and β asarones might be because of synergetic effect. The hepatoprotective activity of essential oils and pure compounds was supported by histopathological examination of livers in which a significant amount of regeneration in the liver was observed. No harmful toxic effects on rats were seen from rhizome The essential oil and major components did not show toxic behaviour. The genetic diversity by Simple sequence repeat (SSR) revealed monomorphic behavior of the species.
Open Access
Studies on alloplasmic and euplasmic heterosis in Brassica juncea L.
(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-11) Rashmi; Ram Bhajan
The present investigation was aimed to estimate heterosis and combining ability in Ogu alloplasmic hybrids as well as euplasmic hybrids, determine specific cyto-nuclear interactions and influence of ogu cytoplasm on hybrid performance. Experimental material consisted of 30 euplasmic hybrids generated by crossing 10 lines viz. PRL-2008-5, PBR-357, Maya, PRKS-28, Rohini, Sej-2, Vaibhav, EJ-22, PYR-2009-5 and PYR-2009-13 and three testers viz. IC-414317, IC-414322 and PR-2006- 14. Besides, same set of lines converted into ogu CMS background was used to develop 30 alloplasmic hybrids by crossing with three restorers (same set of testers carrying restorer gene). The parents, euplasmic and alloplasmic hybrids were evaluated in Compact Family Block Design with three replications during rabi 2016-17 at N.E.B.C.R.C., G.B. Pant University of Agriculture and Technology, Pantnagar. The data recorded on 13 characters was subjected to combining ability analysis (Kempthorne 1957), heterosis over mid parent as well as better parent (Hayes et al., 1955) and difference of mean values of two set of F1’s to determine extent of biological penalty. ANOVA revealed that mean squares due to families were significant for all characters while within family variance were found non-significant only for siliqua density. ANOVA for line × tester performed separately for both sets showed that treatment variance was significant for all characters in both the sets. Further partitioning of total variance into parents, crosses and parents vs crosses revealed that variance due to parents was significant for all characters except 50 % flowering and days to maturity in euplasmic set; and number of primary branches in alloplasmic set. Partitioning of variances due to crosses into lines, testers and line × tester in both sets showed significance mean squares for most of the characters. Mean squares due to testers were significant for number of primary branches, number of secondary branches, yield per plant and 1000 seed weight in alloplasmic set and for number of secondary branches and yield per plant in euplasmic set. For lines in euplasmic set, characters like plant height, length of main raceme, siliqua length and yield per plant showed significant differences while in alloplasmic set only yield per plant exhibited significant mean square. Variances due to line × tester interactions were significant for all characters in alloplasmic as well as in euplasmic set except siliqua length and number of seeds/siliqua in the latter. Based on desirable GCA effects, PBR-357, PYR-2009-5 and EJ-22 were identified as promising lines in euplasmic set while PBR-357A and PRL-2008-5A were high GCA lines in alloplasmic set for seed yield per plant. Among testers, IC-414317 and it’s A-line were found to be good general combiner in euplasmic and alloplasmic sets. The lines PBR-357, Maya, PR-20 in euplasmic and PBR-357A, PR-20A and RohiniA in alloplasmic set were identified as promising donors for oil content. Among testers IC-414322 was found to be good donor for oil content in both sets. Of these PBR-357, was found to have high GCA for seed yield per plant as well as oil content converged with key components. In euplasmic set, two crosses PBR-357 × PR-2006-14 & PR-20 × PR-2006-14 showed significant SCA for seed yield per plant and oil content. On the same analogy, three crosses MayaA × IC-414317, RohiniA × IC-414317 and EJ-22A × IC-414322 in alloplasmic set manifested significant SCA effects for these end products in desirable direction. The heterosis of high magnitude was manifested by large number of crosses for seed yield per plant in euplasmic as well as alloplasmic sets. Maya × IC-414317 manifested highest heterosis followed by Maya × IC-414322 and PBR-357 × PR-2006-14 in both of the sets while PBR-357 × IC-414322 and PBR-357 × PR-2006-14 for oil content were two promising combinations in both sets, respectively. The results revealed considerable amount of biological penalty which varied with cross combination and the characters. All crosses exhibited biological penalty for one or more characters under study. However, five alloplasmic cross combinations viz. PBR-357A × IC414317, PBR-357A × IC-414322, EJ-22A × IC-414322, PYR-2009-5A × IC414317 and PYR-2009-13A × PR-2006-14 performed better than corresponding euplasmic cross combinations for seed yield per plant indicating a positive cytoplasmic effect i.e. effect of cytoplasm was negated by restorer. Such combinations appear promising for commercial hybrid crop production. In the present study, influence of cyto-nuclear interaction was studied by comparing the estimates of GCA, SCA and manifestation of heterosis in euplasmic vis-à-vis in alloplasmic set of crosses. Comparison of GCA, SCA and heterosis estimates for different characters showed that it may or may not be affected by the cyto-nuclear interactions. There was complete change in the GCA status (Good to Poor or Poor to Good) of different lines for 3 or more characters. For example, PBR-357 exhibited changed GCA status for 9 characters, Maya for five characters; and PR-20 for only three characters i.e. siliquae on main raceme, number of secondary branches and siliqua length. GCA status nine lines were consistent in euplasmic as well as in alloplasmic sets for seed yield and six lines for plant height. Similarly visible influence of cyto-nuclear interaction was observed on SCA and heterosis in large number of crosses for different characters.
Open Access
Growth promotory effect of drought tolerant bacteria on wheat crop under drought stress
(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-07) Gouri, Vinita; Singh, Ajay Veer
In natural environmental condition plants continuously exposed to various biotic and abiotic factors. Drought is considered as major abiotic stress and adversely affects plant growth and productivity worldwide. Plants under drought stress conditions show reduced root length, shoot length, deficiency of nutrients and increases disease susceptibility and hence decreases economic yield of crop. In this context, present study was carried out with the application of drought tolerant plant growth promoting rhizobacteria (PGPR) in wheat to promote growth and productivity under drought stress conditions. During study, total sixty bacterial isolates were retrieved and revived from departmental culture collection. On the basis of growth present on tryptic soy broth medium supplemented with 6% poly ethylene glycol and 20% NaCl, fourteen bacterial isolates were selected as drought tolerant. These drought tolerant bacterial isolates were further checked for plant growth promoting activities such as siderophore production, zinc solubilization, phosphate solubilization and ACC deaminase activity and ten best potential bacterial isolates were screened for further study. Afterward, screened potential bacterial isolates were selected and characterized on the basis of cell and colony morphology, biochemical characteristics and carbohydrate utilization tests. Now these potential isolates were characterized functionally and tested for plant growth promoting traits such as exopolysaccaride production, ammonia production, indole acetic acid production and hydrogen cyanide production. Furthermore, potential bacterial isolates were evaluated for antibiosis activity against two pathogenic fungi of wheat, four isolates were able to suppress mycelial growth of Fusarium oxysporum and three bacterial isolates were able to suppress mycelial growth of Alternaria spp. However, SRPII7 isolate was effective against both the fungi. Furthermore, an in situ greenhouse pot experiment was designed under drought stress with all ten drought tolerant plant growth promoting isolates with early variety of wheat (Triticum aestivum var. UP-2785). Results of green house pot experiment confirmed that all bacterial isolates were significantly able to improve plant growth and productivity in terms of agronomical parameters such as root length, shoot length, dry weight of plant, spike length, number of grains/spike, 1000 grains weight and % harvest index, while four bacterial isolates i.e. WRPA13, WRPA26, SRA1 and SRK14 prove more potential compare to all other isolates and helps wheat plant to cope up with drought stresses. In vitro and in situ studies depicted the potentiality of four bacterial isolates for their promising plant growth promoting activity under drought stress. In respect to said results, present study concluded that these four potential bacterial isolates i.e. WRPA13, WRPA26, SRA1 and SRK14 can be employed as bioinoculant/biofertilizer to improve drought tolerance and nutrient enhancement in wheat crop for sustainable agriculture.
Open Access
Feasibility assessment of biological hydrogen production from organic fraction of municipal solid waste using facultative anaerobic bacteria
(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-09) Sharma, Preeti; Melkania, Uma
The rapid consumption of non-renewable fossil fuels has resulted in the environmental pollution and severe energy crisis, which emphasize the importance of renewable biofuel production. Biological hydrogen is a carbon-free renewable energy carrier, with high energy density. Biological hydrogen production methods are less energy intensive, environmental-friendly and sustainable compared to the current energy production methods. Anaerobic digestion is an effective means of organic waste utilization and hydrogen production. At the same time, waste disposal is a problem of the modern times. Most of this waste is dumped in open areas creating severe environmental problems. The organic fraction of municipal solid waste (OFMSW) is highly degradable, thus anaerobic fermentation of OFMSW can be one of the promising methods to generate hydrogen as it is abundant and free of cost. It provides an eco-friendly solution to organic waste by converting waste to biofuel. The present study investigates the feasibility assessment of biological hydrogen production from organic fraction of municipal solid waste. Co-culture of two facultative anaerobic bacteria viz. E. coli and Enterobacter aerogenes were applied for the investigation. Culture conditions were optimized to gain maximum hydrogen production. Various chemical compounds such as surfactants, antifoaming agents, biochar, ferric species etc were applied to improve hydrogen production. Some inhibitors such as furan derivatives, phenolic compounds and heavy metals were also analyzed for their effect on hydrogen production and yield. From the present investigation it can be concluded that proper optimization of fermentation conditions can significantly improve hydrogen production and yield. Based on the findings of the present study it is recommended to further investigate the effect of different substrates and microbial cultures on hydrogen production in order to increase the hydrogen yield.
Open Access
Early detection and management of white rust disease (Albugo candida) in rapeseed mustard
(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-08) Gairola, Kalpana; Tewari, A.K.
Among various diseases reported to occur on rapeseed mustard, white rust caused by Albugo candida is considered as one of the most important disease due to its destructive nature, wide distribution and grain yield losses of 17-34 per cent. The present investigation was carried out with the objectives of: early detection of A. candida, the cause of white rust disease; evaluation of rapeseed-mustard genotypes in field and in glasshouse (at cotyledonary and true leaf stage) and evaluation of some new fungicides for the effective management of the disease. The early detection of A. candida was done by PCR-based assay and light microscopy. In PCR based assay the primers ITS1 (3’-GAGGGACTTTTGGGTAATCA-5’) and Short ITS JV34 (3’- CGCCATTTAGAGGAAGGTGA-5’) and JV37 (3’-GTCAAGCAAAACAT-5’) were used to amplify the ITS region of A. candida and Alternaria brassicae. PCR amplification of A. candida from inoculated symptomatic and asymptomatic leaves yielded PCR products of 1200 bp and 600 bp of ITS1 and Short ITS primers, respectively whereas no bands were amplified in A.brassicae. This confirmed the presence of A. candida in asymptomatic inoculated leaves at early stage i.e. 1, 2, 3, 4, 5 and 6 DAI. In light microscopy the presence of pathogen structures were observed from inoculated symptomatic and asymptomatic inoculated leaves. This presence of pathogen structure viz. mycelium and sporangia was observed in asymptomatic leaves at early stage at 6,7,8 and 9 days after inoculation and from symptomatic leaves at 10 and 11 days after inoculation where as no fungal structure in healthy mustard leaves after staining with 1 percent cotton blue in lacto phenol and 0.4% trypan blue. A large number of rapeseed-mustard materials collected from different sources evaluated in field and in glasshouse (at cotyledonary and true leaf stage) revealed that for the confirmation of resistant sources against white rust disease it is very essential to evaluate Brassica materials first in field and then in glasshouse at both the stages i.e. at cotyledonary and true leaf stage under high disease pressure because some Brassica materials escaped from the disease in field but found susceptible in glasshouse at both the stages (EC-399299) or only at true leaf stage ( Katili local, E. sativus, Basanti and Banarasi rai, PWR-14-8, PWR-14-9, PWR-14-10, PWR-14-11, RMT-1-10-1, IC 597942 and IC265495). Among various fungicides Metalaxyl 8% + Mancozeb 64% (Ridomil MZ @ 0.25%) and a biological origin Azoxystrobin (Amistar 25 EC @ 0.1%) were found highly effective in inhibiting sporangial germination in-vitro and were found highly effective in controlling white rust disease (no occurrence of disease) in glasshouse and field in increasing grain yield and test weight followed by Propiconazole, Tebuconazole+Trifloxystrobin, Trifloxystrobin, Kresosim methyl (each at 0.1%). Garlic bulb extracts (2%) was also found effective in managing the disease even better than some old recommended fungicides
Open Access
Bayesian estimation in weighted x gamma distribution
(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-12) Agrawal, Priya; Vinod Kumar
In the present study, a new lifetime distribution, named weighted xgamma distribution has been proposed and its distributional properties are investigated. The maximum likelihood estimates of the parameter θ have been obtained by means of Newton-Raphson method. The expressions for various distributional properties of weighted xgamma distribution including its moment generating function (mgf), cumulant generating function (cgf), characteristic function, moments etc. have been derived. The Bayes estimators of its parameter (θ), reliability function R(t) and hazard rate function h(t) are obtained using Tierney and Kadane method of approximation under two priors namely uniform and gamma. The results obtained have been illustrated by means of several randomly generated data sets from the proposed model, each sample replicated 10,000 times The Bayes Risks have been evaluated by using Squared Error Loss Function (SELF). A real life data set has also been used to establish its utility. It is concluded that gamma prior is superior to uniform prior for finding Bayes estimates of the parameter θ, reliability function R(t) and hazard rate function h(t) of the proposed weighted xgamma distribution.

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