Loading...
Theses
Browse
2 results
Search Results
ThesisItem Open Access Studies on regenerative potential of hepatocytic mesenchymal cells in liver degeneration and delineation of their gene expression in canine(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2019-09) Saini, Rashmi; Jadon, N.S.Twenty four dogs of either sex naturally suffering from hepatic dysfunction were used in this study. The animals were randomly divided into four groups administered with different drug or drugs combination viz. control (Group A); pepsid-C @ 1ml /10 kg body weight, IM alternatively for fifteen days (Group B); hepatocytic stem cells @ 5×107 cells, intraperitonially animal thrice at an interval of 5 days (Group C) and hepatocytic stem cells @ 5× 107 cells, intraperitonially animal thrice at an interval of 5 days + pepsid-C @ 1ml /10 kg body weight, IM alternatively for fifteen days (Group D). The clinical signs, haematological (Hb, TEC, TLC, DLC, PCV, BT and CT), biochemical (serum T.P, serum albumin, serum total bilirubin, serum glucose, serum urea nitrogen, serum creatinine and serum AST, serum ALT and serum GGT) parameters were studied at 0, 3rd, 6th, 10th, 15th and 20th day by using standard protocol. Radiographic and ultrasonographic changes were studied at 0 and 20th day following treatment. Gene expression of CD44 gene was evaluated via real time PCR. On the basis of parameter observed in this study, it was concluded that the effectiveness of treatment at 15th and 20th day interval was more in the animals treated with combination of hepatocytic stem cells + pepsid-C (Group D) and hepatocytic stem cells (Group C) alone as compared to pepsid-C alone (Group B), as indicated by haematological, biochemical, radiographic, ultrasonographic examination and gene expression of CD44 studies. Hepatocytic stem cells and pepsid-C are safe drugs used in the present study as there was no adverse biochemical and hematological changes during entire period of study. However, the variable changes observed during these periods were purely temporarily and were within normal physiological limits. Ultrasonographic study at day 0 interval of all the animals showed various degree of increased echogenicity, hepatopathy, changes in hepatic symmetric or asymmetric, cholecystitis, acute hepatitis and chronic hepatitis. Variable regenerative changes were seen in rest of the three groups B, C and D at 20th day following the administered of pepsid-C alone, hepatocytic stem cells alone and combination of hepatocytic stem cells + pepsid-C respectively. Abdominal radiograph of all the animals of group A, B, C and D at day 0 interval revealed the morphologic abnormalities in size, shape, position, and radio density of the liver. Gene expression of CD44 associated with regeneration was evident in animals where liver derived hepatocytes were administered and not evident in other group (A and B) while GAPDH, being a housekeeping gene was evident in all groups. Hepatocytic stem cells are necessary for the creation of new cells for growth, development and regeneration. These cells are responsible for the development of new specific cells and also have potential to differentiate into different cell types but are mostly bipotent and are specific for the type of tissue they belong to. These hepatocytic stem cells have better effect on liver function. Thus the combination of hepatocytic stem cells and pepsid-C may be used safely by field veterinarian for the treatment of hepatic dysfunction in canine.ThesisItem Open Access Studies on effects of thiopental sodium with and without dexmedetomidine and its reversal with yohimbine in dogs(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2016-07) Saini, Rashmi; Jadon, N.S.