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Author: Pandey, Nitin Kumar × End date: 2009 × Start date: 2009 ×
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    ThesisItemOpen Access
    Analysis of economic traits and expression profile of different growth related genes in IGF-1 SNP genotypes of Indian colored broilers
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-06) Pandey, Nitin Kumar; Sharma, R.K.
    IGFs are involved in metabolism and body growth by regulating the synthesis and degradation of glycogen and proteins in animals. In chicken IGF-1 gene, an A570C substitution has been reported to invariably associate with growth, body composition, skeletal integrity, hormonal and metabolic factors. The present study aimed to detect polymorphism in the respective region (promoter and 5’ UTR) in CSML individuals in two generations using Hinf I PCR-RFLP. An array of economic traits viz. Juvenile body weights, Body conformation traits, Carcass traits and crude protein% in breast muscle were recorded on birds of different genotypes of IGF-1. Expression analysis of various growth related genes i.e. IGF-1, IGF-2, IGF-1 receptor, Growth hormone receptor, IGFBP-2, Ghrelin, Myostatin and TGF-β2 were also carried out using real time PCR in liver and breast muscle samples of birds of each of IGF-1 genotype at 45 days of age. Genomic DNA has been isolated from blood using phenol choloroform extraction method. A total of 100 and 96 birds were screened for estimating gene and genotypic frequencies in two successive generations. PCR amplified fragment of 813bp was digested with Hinf I. The Hinf I digestion revealed patterns viz. AA (378, 244 and 191bp), BB (622 and 191bp) and AB (622, 378, 244 and 191bp) in both the generations. The overall frequency of AA, AB and BB genotypes were 0.74, 0.23 and 0.03; in generation I these were 0.70, 0.27 and 0.03; and in generation II 0.78, 0.18 and 0.04, respectively. The frequency of allele A and B in generation I was 0.83 and 0.17 and in II generation 0.87 and 0.13, respectively, with an overall frequency of A and B alleles as 0.85 and 0.15, respectively. Significant differences among IGF-1 genotypes were observed on body weights at III, IV and V weeks of age in first generation and on body weight at V and VI weeks of age in II generation. IGF-1 AA and IGF-1 AB genotype exhibited higher body weights than IGF-1 BB genotype birds. Significant effect of IGF-1 genotypes on conformation traits was observed in both the generations. IGF-1 AA and IGF-AB genotypes had significant effect on all the carcass traits except EY%, heart, gizzard, giblet and abdominal fat in first generation and EY%, spleen and bursa in II generation. For cutup parts IGF-1 genotype had significant effect on back, thigh, drumstick and wing in first generation and on all the cut-up parts except back weight in II generation. IGF-1 AA genotype birds exhibited significantly higher CP% in breast muscle as compared to that IGF-1 AB genotype birds. Expression profile of all the genes studied showed pattern of higher expression in hepatic tissue of AB and AA genotypes as compare to BB genotype. However, except myostatin, the picture was reverse in PM muscle, where expression was higher in IGF-1 BB genotype.
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    ThesisItemOpen Access
    Analysis of economic traits and expression profile of different growth related genes in IGF-1 SNP genotypes of Indian colored broilers
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-06) Pandey, Nitin Kumar; Sharma, R.K.
    IGFs are involved in metabolism and body growth by regulating the synthesis and degradation of glycogen and proteins in animals. In chicken IGF-1 gene, an A570C substitution has been reported to invariably associate with growth, body composition, skeletal integrity, hormonal and metabolic factors. The present study aimed to detect polymorphism in the respective region (promoter and 5’ UTR) in CSML individuals in two generations using Hinf I PCR-RFLP. An array of economic traits viz. Juvenile body weights, Body conformation traits, Carcass traits and crude protein% in breast muscle were recorded on birds of different genotypes of IGF-1. Expression analysis of various growth related genes i.e. IGF-1, IGF-2, IGF-1 receptor, Growth hormone receptor, IGFBP-2, Ghrelin, Myostatin and TGF-β2 were also carried out using real time PCR in liver and breast muscle samples of birds of each of IGF-1 genotype at 45 days of age. Genomic DNA has been isolated from blood using phenol choloroform extraction method. A total of 100 and 96 birds were screened for estimating gene and genotypic frequencies in two successive generations. PCR amplified fragment of 813bp was digested with Hinf I. The Hinf I digestion revealed patterns viz. AA (378, 244 and 191bp), BB (622 and 191bp) and AB (622, 378, 244 and 191bp) in both the generations. The overall frequency of AA, AB and BB genotypes were 0.74, 0.23 and 0.03; in generation I these were 0.70, 0.27 and 0.03; and in generation II 0.78, 0.18 and 0.04, respectively. The frequency of allele A and B in generation I was 0.83 and 0.17 and in II generation 0.87 and 0.13, respectively, with an overall frequency of A and B alleles as 0.85 and 0.15, respectively. Significant differences among IGF-1 genotypes were observed on body weights at III, IV and V weeks of age in first generation and on body weight at V and VI weeks of age in II generation. IGF-1 AA and IGF-1 AB genotype exhibited higher body weights than IGF-1 BB genotype birds. Significant effect of IGF-1 genotypes on conformation traits was observed in both the generations. IGF-1 AA and IGF-AB genotypes had significant effect on all the carcass traits except EY%, heart, gizzard, giblet and abdominal fat in first generation and EY%, spleen and bursa in II generation. For cutup parts IGF-1 genotype had significant effect on back, thigh, drumstick and wing in first generation and on all the cut-up parts except back weight in II generation. IGF-1 AA genotype birds exhibited significantly higher CP% in breast muscle as compared to that IGF-1 AB genotype birds. Expression profile of all the genes studied showed pattern of higher expression in hepatic tissue of AB and AA genotypes as compare to BB genotype. However, except myostatin, the picture was reverse in PM muscle, where expression was higher in IGF-1 BB genotype.