Loading...
Theses
Browse
1 results
Search Results
ThesisItem Open Access Studies on cryopreservation of Pantja buck semen(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2020-11) Dhara, Souvik; Gupta, H.P.Present study was conducted with the objectives of evaluation of seminal attributes and development of suitable extender for cryopreservation of Pantja buck semen. The experiment was divided in two parts as Experiment I and experiment II. For experiment I, the ejaculates from four sexually mature bucks were pooled and divided into three sub samples A, B, C and D. Each sub samples were diluted in tris based medium, supplemented with varying concentration of soybean lecithin like, A (0.1% SL), B (0.2% SL), C (0.3% SL) and D (0.4% SL). The sub samples were examined for progressive motility, plasma membrane integrity, liveability and acrosome integrity at post-dilution, post-equilibration and post-thawing stages. For experiment II, ejaculates from four sexually mature bucks were pooled after evaluation of each sample separately. Pooled semen was divided into three equal aliquots as I, II and III and rest portion was centrifuged to separate seminal plasma for bio-chemical evaluation. Group I sample was diluted into two parts as D1 [Neat semen+ tris-fructose-citric acid-egg yolk (7.5%)-glycerol] (n=8) and D2 [Neat semen+ tris-fructose-citric acid-Soya lecithin (0.2%)-glycerol] (n=8). Group II was centrifuged to removal of seminal plasma and diluted into two parts as D3 [Washed semen+ tris-citric acid-egg yolk (7.5%) glycerol] and D4 [Washed semen + tris-citric acid- SL (0.2%)-glycerol]. Again group III sample was centrifuged to remove the seminal plasma and diluted into two parts as D5 [Washed semen + tris- citric acid-egg yolk (7.5%) - Bovine seminal plasma- glycerol] and D6 [Washed semen + tris- citric acid-SL (7.5%) - Bovine seminal plasma- glycerol]. Each sample was examined for progressive motility, liveability, plasma membrane integrity, acrosome integrity and morphological abnormality at postdilution, post-equilibration and post-thawing stages. Evaluation for Malondialdehyde (MDA) assay, Glutamic Oxaloacetic Transaminase (GOT), Glutamic Pyruvate Transaminase (GPT) and Glutathione Peroxidase (GSH-Px) were done at post-thawing stage. A total 12 (6 from group D1 and 6 from group D6) does were artificially inseminated by frozen thawed semen to evaluate fertility rate. The mean value of seminal attributes of four Pantja bucks were volume 0.36±0.06 ml, pH 6.86±0.04, mass motility (0-5 Scale) 4.47±0.22, progressive motility 92.28±1.17%, concentration 3.54±0.14 x 109/ ml, liveability 87.28±0.78%, acrosome integrity 94.31±1.05%, plasma membrane integrity 90.27±0.86%, head abnormality 1.64±0.12%, mid-piece abnormality 0.34±0.04%, tail abnormality 2.76±0.10%, total abnormality 4.70±0.17%, GPT 18.10±1.20 U/L, GOT 124.04±8.48 Un/L and GSH-Px 10.90±0.53 U/ml. In experiment I, group B (0.2% SL) showed significantly higher value of post-thawed seminal attributes compared to other groups (p< 0.05). In experiment II, Significant difference was observed in seminal attributes between soybean lecithin based extender and egg yolk based extender, washed and nonwashed semen (p< 0.05). Significantly higher post-thawing seminal parameters were observed in group D6 compared to rest of the groups (p< 0.05). In both of the experiments significant reduction of seminal attributes were observed at each steps of freezing (p< 0.05). Conception rate of group D1 and D6 were 33.33% and 66.67% respectively.
