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Author: Bhatt, Pooja × End date: 2009 × Start date: 2009 × Type: Thesis ×
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    ThesisItemOpen Access
    Effect of mode of administration on hematological parameters and biochemical profile of Labeo rohita (Ham.) fingerlings exposed to mercuric chloride
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-08) Bhatt, Pooja; Ram, R.N.
    The present investigation was aimed at to find out the mode of administration dependent variation in hematological parameters (Hb level, TEC, TLC, DLC, Ht percent, MCV, MCH and MCHC) and biochemical profile (Moisture, Protein, Lipid, Ash and NFE) of Labeo rohita fingerlings when they were exposed to HgCl2. The experiment was performed in two phases, acute and chronic. For acute toxicity assay Labeo rohita fingerlings were exposed to different doses of mercuric chloride at the rate of 0.6, 0.4 and 0.2 mg/l through water and 0.6, 0.4 and 0.2 mg/kg through feed for 96 hrs. In a different set of acute toxicity experiment, different sizes of Labeo rohita viz. 18mg, 8.5mm; 49mg, 18mm and 220mg, 40mm were exposed to a single dose i.e. 0.1 mg/l through water and 0.1 mg/kg through feed for 168 hrs. For chronic toxicity assay, Labeo rohita fingerlings were exposed to HgCl2 for 49 days both via water (0.05mg/l) and via feed (0.05mg/kg feed) and assessment of different hematological parameters and biochemical profile was done on 0th, 7th, 21st and 49th day. The results of acute toxicity assay revealed that the hematological parameters and biochemical profile were affected by dose, size and mode of administration. Younger fishes were more vulnerable to mercuric chloride and fishes showed major responses toward higher doses. Significant differences were observed in the mode of administration dependent variation in hematological and biochemical parameters except in muscle protein (96hrs exposure) and in basophil count, MCHC, moisture and NFE (168hrs exposure). Chronic exposure assay established that the dietary mercuric chloride pose more health hazard to fish than the mercuric chloride administered through water.