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ThesisItem Open Access Morphological and molecular diversity in sorghum (Sorghum bicolor (L.) Moench) genotypes in relation to grain mold(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-08) Amit Kumar; Vishunavat, KarunaThe present investigation on “Morphological and molecular diversity in sorghum (Sorghum bicolor (L.) Moench) genotypes in relation to grain mold” was carried out Crop Research Centre and Seed Pathology Laboratory, Department of Plant Pathology, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttrakahand, India, during the year 2005-07 with twenty six genotypes. The investigation was undertaken to study the screening of sorghum genotypes for grain mold, analysis of means and variance for plant and seeds parameter, biochemical and molecular characterization of sorghum cultivars in relation to grain mold and Inter-character correlation for plant, seeds and mold incidence. Twenty six sorghum genotypes were categorized into five groups for the mold infestation on the basis of visual assessment which revealed that three varieties viz. HC-136, MP Chari Red and GFS-4 were found to be highly resistant, which was further conformed by the blotter method. The analysis of variance revealed significant differences among the varieties for the quantitative characteristics. Higher estimates of phenotypic and genotypic variance were recorded for the characters viz. total height of the plant at maturity, natural height of the foliage up to base of flag leaf, time of panicle emergence and the length of panicle without peduncle. The PCV and GCV were of the same magnitudes for majority of the characters. However, relatively higher magnitude of difference between PCV and GCV was observed for three characters viz., anther length, visible length of panicle neck above the sheath and 1000 seed weight indicating the greater role of environment in their expression. The present experimental material possess high heritability coupled with high to low moderate genetic advance for majority of the characters except for the character anther length indicating substantial proportion of additive genetic variance in their expression thereby, confirming their consistency over the years. Among the seed quality parameters high significant correlation was observed between seed weight per 25 seeds with seed density and volume per 25 seeds. Weight per 25 seeds with vigour index I, germination percentage with vigour index I and vigour index II. Association studies between mold incidence and seed quality parameters revealed significant positive correlation between electrical conductivity with mold incidence. However, quality parameters viz., density per seed and percent germination showed negative but significant correlation with mold incidence. Out of a total of 14 quantitative plant parameters only one characters viz., anther length showed significant and positive correlation with mold incidence. Inter-correlation among fungal complex (Curvularia lunata, Fusarium moniliforme and Phoma sorghina) were mostly positive. Seed storage protein profiling and RAPD analysis revealed that clustering of the varieties could roughly be coincided with their mold infestation percentage at laboratory conditions.