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ThesisItem Open Access Seasonal influence on physiological, haemato-biochemical and milk parameters of lactating indigenous Badri cows(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2022-06) Patel, Mayankkumar Kanaiyalal; Rastogi, S.K.Badri is a dual purpose first registered cattle breed of Uttarakhand which was earlier known as the „Pahadi‟ cattle. This breed is mainly reared in hilly areas of Garhwal and Kumaon divisions in Uttarakhand state. The breed is famous for its adaptability to hilly terrain, disease resistance ability and medicinal properties of milk, urine and dung. This study aimed to investigate seasonal influence on physiological, haemato-biochemical and milk parameters of lactating indigenous Badri cows. Eleven healthy lactating Badri cows were selected randomly from Instructional Dairy Farm of GBPUAT, Pantnagar. The entire study period was conducted in 3 seasons viz. monsoon season (July 2021-September 2021), winter season (December 2021-January 2022) and spring season (February 2022-March 2022). Meteorological variables, physiological parameters and body condition score were recorded during each season. Blood samples were collected during each season for analysis of various haemato-biochemical parameters. Fresh milk samples were subjected to analysis for milk composition and somatic cell count during different seasons. Meteorological variables recorded during study period gave higher THI during monsoon than winter and spring. Respiration rate, rectal temperature and pulse rate were significantly increased as the ambient temperature increased. BCS was significantly higher during winter season as compared to monsoon season. Among haematological entities, Hb, PCV and TEC were significantly higher during winter season whereas TLC, MCHC, neutrophil percentage, absolute neutrophil count and N:L ratio were significantly higher during monsoon season. Erythrocyte osmotic fragility at 0.5% saline concentration was significantly higher during monsoon season as compared to winter season. Among serum biochemical entities, total protein, albumin, cholesterol and glucose concentrations were significantly higher during winter season as compared to monsoon season. Average values of serum urea and creatinine were significantly higher in monsoon season whereas calcium and phosphorus were higher during spring season. Milk fat, SNF, protein and total solid percentage were significantly higher during winter season as compared to monsoon season. Milk SCC was significantly higher during monsoon season as compared to winter season. Among milk somatic cells, neutrophil was significantly increased in monsoon season as compared to spring season. The changes in physiological, haemato-biochemical and milk parameters indicated that monsoon season was more stressful to lactating Badri cattle. Therefore, better care and management should be taken during monsoon season for the protection of animals against heat stress. The present study also indicated that lactating Badri cattle were well adapted to extreme cold climatic conditions of winter season.ThesisItem Open Access Comparative studies on hematology, milk composition and udder morphology of lactating Sahiwal and crossbred cattle(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-09) Maiti, Abhinaba; Huozha, R.The current study has been undertaken to investigate various hematological entities, milk composition and udder morphology between healthy lactating Sahiwal and crossbred cattle. Total twenty healthy Sahiwal and crossbred cattle of 2nd to 3th parity were selected from the University Instructional Dairy Farm for this present study. Selected animals were kept as normal lactating animals in the farm where both blood and milk samples were collected once and followed their lactation stages as early (5-90 days), mid (91-180 days) and late (181-270 days). Among hematological entities Hb, PCV, TLC, erythrocytic indices, and both DLC (neutrophil, lymphocyte) and ALC (eosinophil) differ significantly during different stages of lactation in both the breeds. Comparing between two breeds during lactation Hb, PCV, TEC, TLC, DLC (lymphocyte, monocyte) and ALC (lymphocyte) were significantly different. Milk quantity as TDMY during different lactation stages found non-significant in Sahiwal, but in crossbreed cattle showed significantly higher during early lactation stage and overall mean than Sahiwal cattle. Among milk composition fat% and total solid% varies significantly during different lactation stages in both the breeds. While SNF%, protein% and lactose% varied significantly during lactation stages in Sahiwal cattle. No significant difference observed between two breed comparisons except in fat% and total solid%. Milk SCC also varied significantly during different stages of lactation in both the cattle breed. Crossbreed cattle had significantly higher SCC than Sahiwal. Among milk somatic cells, neutrophil% and lymphocyte% changed significantly during lactation stages in both the breed as well as between two breeds. Udder morphology had shown significant effect on milk SCC, milk neutrophils and TDMY but non-significant effect on milk composition. Round shaped udder and cylindrical shaped teat was found having higher milk SCC among others and trough shaped udder and conical shaped teat had least milk SCC in both the breeds. Correlation study between udder measurements with milk SCC showed that height from the ground to teat apex, teat diameter, average teat length are significantly correlated with milk SCC. Overall comparison between lactating Sahiwal and crossbred cattle with respect to effect of lactation stages indicated that indigenous Sahiwal cattle having better erythrocyte entities, blood DLC, and milk SCC and DLC with superior milk quality though TDMY was much lesser than crossbreed cattle. Thus, concluded that different stages of lactation highly affected the normal physiological state in both the breed and milk SCC changes according to lactation stages, udder and teat morphology.ThesisItem Open Access Herbal formulations augmenting physio-biochemical, serological and carcass quality entities in commercial broilers(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-03) Verma, Megha; Rastogi, S.K.A study was conducted in 180day old broilers (divided into three groups having four replicates of 15 birds in each). The experiment was carried out to evaluate the effect of herbal formulations augmenting physio biochemical, serological and carcass quality entities in commercial broilers. The study was conducted during months of Aug- Oct 2019. Herbal formulations viz., AV/HLP/16 and AV/LMP/10 were supplemented to treatment 1 and treatment 2 @ 500g/ tonnes of feeds respectively, where as in group control no herbal supplements were added. At the end of the study, different parameters were studied such as body weight gain, feed intake, physio-biochemical, serological, carcass quality and gene expression study. The body weight gain was higher in T1 group in comparison to T2 and control group. The feed intake was found significantly lower in T1 group fed AV/HLP/16 in comparison to T2 group fed AV/LMP/10 and control group. The better feed efficiency was recorded in T1 group as compared to T2 and control. Various parameters such as liveability percentage, relative growth rate, European efficiency factor, Protein efficiency ratio and energy efficiency ratio was also better in T1 group followed by T2 group in comparison to control group. The haematological parameters such as TLC, TEC, PCV and leucocytes count were insignificant among all the group but Hb% was slightly higher in T1 group as compared to T2 and control. The osmotic fragility was also improved in T1 group in comparison to T2 and control group. But AV/HLP/16 group showed slightly improvement in lymphocyte count due to immunomodulatory activity followed by AV/LMP/10 in comparison to control. Serum biochemical profile suggested that addition of herbal supplements in T1 and T2 groups caused significant decreased in cholesterol, triglycerides, LDL, A: G ratio and glucose content but there was a significant increase seen in the concentration of serum protein, HDL and globulin. It was also noted that supplementation of AV/HLP/16 improved the DM, EE, and CP (%) digestibility in comparison to AV/LMP/10 and control group. The carcass characteristics, organoleptic properties and chemical composition of carcass reflected pH, CP, EE and DM (%) was found better in T1 group in comparison to T2 and control group. The cell mediated and humoral immunity against NDV virus was also found increased in T1 group fed AV/HLP/16 followed by AV/LMP/10 and control group. Based on the antioxidative profile there was decreased mean MDA value in T1 group as compared to T2 and control group whereas increased profile of SOD and catalase unit in T1 group as compared to T2 and control. The histopathological examination of liver and muscle tissue revealed no significant findings. The relative mRNA expression of genes related to lipid formation i.e., ACC and FAS were non significantly downregulated in T1 and T2 group in comparison to control. The relative expression of SREBP-1c gene was significantly downregulated (p<0.05) in T1 and T2 group than control. Also it was found that CPT-I and PPAR α (involved in lipid peroxidation) were non significantly higher in T1 and T2 group than control. The above study, therefore suggested that supplementation of AV/HLP/16 in T1 group performed better due to antioxidant effect of components
