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Subject: Veterinary Pharmacology and Toxicology × Author: Naveen Kumar × End date: 2024 × Start date: 2020 × Type: Thesis ×
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    ThesisItemOpen Access
    Evaluation of antioxidative and immunomodulatory potential of Trigonella foenum graecum against imidacloprid induced toxicity in rats
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-01) Naveen Kumar; Ahmad, A.H.
    The study was carried out to investigate the ameliorating potential of Trigonella foenum graecum in combating imidacloprid induced immunotoxicity and oxidative stress in adult male Wistar albino rats. Rats were divided into five groups of eight rats each. In group I, vehicle (olive oil), group II, imidacloprid @ 170 ppm, group III, aqueous extract of seeds of Trigonella foenum graecum alone @ 12000 ppm, group IV imidacloprid @ 170 ppm and diosgenin @ 2400 ppm, and group V, imidacloprid @ 170 ppm and aqueous extract of Trigonella foenum graecum @ 12000 ppm were administered daily with feed to the rats orally for 90 days.The results of qualitative phytochemical analysis revealed presence of alkaloids, saponins, flavonoids, phenolic compounds and carbohydrates. The DPPH and ABTS radical scavenging assays for aqueous extracts were also found to be highest in seed extract. The rate of gain in body weight was significantly decreased in imidacloprid group, however, in combination with fenugreek and diosgenin it was restored to normal level. Imidacloprid exposure caused significant increase in serum level of ALT, AST, ALP and creatinine activities as compared to all other groups. Treatment with imidacloprid significantly decreased the RBC, Hb, PCV values and was significantly lower than either of the treatment. Imidacloprid produced toxicity in the form of enhanced lipid peroxidation and reduced GSH, SOD and catalase levels. Trigonella foenum graecum was significantly effective in restoration of these parameters towards normal. ROS generation was significantly increased following exposure to imidacloprid as compared to control. Significant (p<0.05) improvement in these parameters were observed in groups where imidacloprid along with fenugreek and diosgenin was administered. Total immunoglobulin, immunoglobulin G and foot pad thickness were significantly increased in imidacloprid group in comparison to co-treatment groups of imidacloprid with fenugreek and diosgenin control group. Thepresent study summarizes that the aqueous extract of Trigonella foenum graecum seeds and diosgenin exert ameliorative effects on imidacloprid induced toxic effects which may attribute to their antioxidative and immunomodulatory properties.