Loading...
Theses
Browse
1 results
Search Results
ThesisItem Open Access Evaluation of protective efficacy of Amaranthus hypochondriacus seed powder in arsenic intoxicated wlh cockerels(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2020-10) Aswal, Renuka; Singh, S.P.The aim of this study was to evaluate the protective and antioxidant potential of seed powder of Amaranthus hypochondriacus(SPAH) following oral administration @ 8000ppm in simultaneously fed arsenic(AR) @110ppm in feed for 60 days in WLH cockerels. Methanol extract of the seed powder of Amaranthus hypochondriacus was also prepared for phytochemical analysis and in vitro antioxidant analysis. The phytochemical analysis of methanol seed extract of Amaranthus hypochondriacus revealed 3.72% yield and the presence of alkaloids, flavonoids, phenolic compounds, sugars, glycosides, proteins and saponins. Thirty WLH cockerels were equally and randomly divided into 5 groups with 6 birds in each group. Group I served as control and group II arsenic@110ppm, group III SPAH@8000ppm, group IV AR@110ppm plus silymarin@100ppm and group V AR@110ppm plus SPAH@8000ppm served as treatment groups for 60 days of feeding trial study. Arsenic causes no noticeable clinical signs in the appearance and behavior of WLH cockerels. Arsenic caused significant (P<0.5) reduction in Hb, PCV, TEC and TLC as compared to control group I. Amaranth alone group III was at par with control group I and has potentiating effect on haematological parameters. A significant (P<0.05) decline in total serum protein, albumin and globulin were observed in group II as compared to control group I. SPAH treated groups V showed significant (P<0.05) amelioration in the level of total serum proteins as compared to group II and at par with silymarin and control groups I ,respectively.A significant (P<0.05) increase in values of triglycerides, cholesterol, creatinine, BUN, AST, ALT, bilirubin was observed in arsenic treated group II, which were restored by SPAH group V towards normal. Group III revealed values of most of the biochemical parameters was at par with control group I and showed potentiating effect of SPAH when given alone .A significant (P<0.05) decline in tissue GSH, SOD and catalase activity and increase in LPO activity was observed in arsenic treated group II and a significant amelioration in antioxidant parameters were observed in arsenic plus SPAH treated group V as par with control group I indicating potent antioxidant effect of SPAH following oral administration for 60 days in birds. Antioxidant activity of methanolic seed extract was also observed in vitro DPPH and ABST free radical scavenging activity in this study. Thus, it is concluded from the present study that arsenic @110ppm produced hemotoxic, hepatotoxic and nephrotoxic effect after 60 days treatment in WLH cockerels. Seed powder of A. hypochondrioacus (SPAH)@8000ppm in feed for 60 days in showed hepatoprotective, nephroprotective and antioxidant potential in WLH cockerels.
