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20172
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Subject: Veterinary Gynaecology and Obstetrics × End date: 2017 × Start date: 2017 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Studies on therapeutic efficacy of various immunomodulators on endometritis in repeat breeding crossbred cows
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-12) Singh, Bhoopendra; Gupta, H.P.
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    ThesisItemOpen Access
    Follicular development, superovulation and embryo recovery following insulin and insulin like growth factor-I (IGF-I) treatment during mid Luteal phase of Estrous cycle in embryo donor and hormonal profile in recipient cattle
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-02) Sheetal, Shailendra Kishore; Prasad, Shiv
    The present study was designed to study the effect of insulin and insulin like growth factor-I (IGF-I) administration during mid luteal phase of estrous cylce on follicular development, superovulation, embryo recovery and alteration in hormonal and biochemical profiles in embryo donor cows. Sahiwal cows (n=18) were selected and divided into 3 groups; control, T-I (Insulin @ 0.25 IU/kg b. wt. S/C on 5, 6, 7 and 8 days of estous cycle ) and T-II (IGF-I @ 10 μg total dose per day S/C on 5, 6, 7 and 8 days of estous cycle). Blood samples were collected on day 5, 7, 9, 11, 13, 15, 17, 19 and 21st or day of embryo recovery (DER). Further, crossbred embryo recipient cows (n=30) were selected and divided into 3 groups; control, T-I (Insulin @ 0.25 IU/kg b. wt. S/C on 0, 1, 2 and 3 days of EC) and T-II (Insulin (@ 0.25 IU/kg b. wt. S/C on 4, 5, 6 and 7 days of EC). Blood samples were collected on 5, 7, 14, 21st days of estrous cycle. The serum was separated and stored at -20°c in refrigerator till analysis of hormonal and biochemical profiles. The mean diameters (mm) of largest follicles and second largest follicles in control, T-I and T-II groups differed non-significantly (P>0.05) between groups on 9, 11 and 13th day of superovulatory treatments, respectively. The ovulation rate, total number of embryos/ova recovered, transferable embryos, degenerated embryos, unfertilized ova did not differ significantly (P>0.05) between the groups. However, the higher embryo recovery rate of 38.46% was recorded in T-I group, than in T-II (35.71%) and lowest in control (31.76%) group. The overall embryo recovery rate of all groups was 35.38%. The mean concentration of progesterone on day 7, 11, insulin on day 7, 9, IGF-I on day 5, 7, 9, 11, 15 and creatinine on day 5, 7, 9, were significantly higher in T-I group as compared to untreated control. However, glucose, cholesterol, total protein and urea did not differ. In recipients, the progesterone level increased significantly (P<0.05) on days 7 and 14 in T-II group, while glucose and urea decreased. The serum insulin, IGF-I, total protein, cholesterol and creatinine differed non-significantly. On the basis of above findings, it may be concluded that administration of insulin during mid luteal phase of estrous cycle may yield better follicular development, superovulatory response, and embryonic development and might be helpful for conception in embryo recipients as compared to untreated control.