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ThesisItem Open Access Studies of qualitative and quantitative characters of okra [Abelmoschus esculentus (L). Moench] genotypes(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-08) Arvind Kumar; Singh, Durvesh KumarThesisItem Open Access Study on potato (Solanum tuberosum L.) based crop sequences under rainfed mid-hill condition of Uttarakhand(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-07) Kipgen, Chinpilhing; Uniyal, S.P.To evaluate eight potato based crop sequences viz., potato-carrot, potato-turnip, potato- radish, potato-cauliflower, potato-cabbage, potato-broccoli, potato-pea and potato-amaranthus, an investigation was carried out at Vegetable Science Research unit of Hill Campus Ranichauri (G. B. Pant University of Agriculture & Technology), Tehri Garhwal (Uttarakhand) during spring-summer (potato), rainy (carrot, turnip, radish, cauliflower, cabbage) and rainy-autumn (broccoli, pea and amaranthus) season, 2008. The experiment was laid out in Randomized Complete Block Design with three replications. During the study, the yields of individual crops were recorded and based on selling prices, potato equivalent yield (PEY), production efficiency (PE), land use efficiency (LUE) and economics of the sequences were worked out. In order to assess the residual effect of first crop (potato) on subsequent crops of the sequences, soil analysis to different soil parameters (pH, organic matter, available N, P, and K) was also performed. Findings of the investigation revealed that amongst eight sequences, potato-radish proved the best in respect to potato equivalent yield (626.66 qha-1) and net profit (Rs. 1,35,241.70 ha-1). This sequence had recorded an additional net income of Rs. 90,700.70 ha-1 over the conventional sequence, potato-amaranthus. In addition, the other sequences which showed higher profitability in the investigation were potato-cabbage (Rs. 1,07,041.70 ha-1), potato-broccoli (Rs. 1,04,175.00 ha-1), potato-carrot (Rs. 91,375.00 ha-1) and potato-pea (Rs. 85,191.66 ha-1) in decreasing order. Based on the overall performance, it can be concluded that under the prevalent climatic/ cultivation conditions of mid hill rainfed Uttarakhand, potato-radish is the most profitable/suited sequence, hence, recommended for farmer‟s commercial cultivation.ThesisItem Open Access Evaluation of new potato (Solanum tuberosum L.) hybrids under mid hill rainfed condition of Uttarakhand(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-01) Raturi, Harish Chandra; Uniyal, S.P.ThesisItem Open Access Heterosis breeding and protein profiling through sds-page in brinjal (Solanum melongena L.)(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-03) Kumar Bharat Bhushan; Singh, Y.V.Present investigation was carried out during autumn winter season 2007-08 and 2008-09 at the Vegetable Research Centre of GBPUA&T, Pantnagar with 36 genotypes including 12 lines, 2 testers and 24 F1s of brinjal. The experiment was laid out in randomized block design with three replications. The observations were recorded for 12 characters viz. days to 50 % flowering, number of primary branches per plant, plant height (cm), fruit length (cm), fruit diameter (cm), number of marketable fruits per plant, weight of marketable fruits per plant (kg), number of unmarketable fruits per plant, weight of unmarketable fruits per plant (kg), total number of fruits per plant, total yield per plant (kg) and total yield (q/ha). The data were subjected to appropriate statistical analysis. Further, seed protein of 14 parents was utilized for sodium dodecayl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The analysis of variance revealed highly significant differences among genotypes for all the attributes under study. High heritability coupled with high genetic advance as per cent of mean was recorded for fruit length indicating importance of additive gene action controlling this character. The F1 cross PR × PS was best heterotic combination for total weight of fruits per plant and yield per hectare (q) over better parent. BARI × PS was best heterotic cross over mid parent and standard parent. Other combinations showing significant values for all types of heterosis were PB 69 × PS, Punjab Sadabahar × PU, PB 66 × PS, PB 64 × PS and PB 69 × PU. The findings of present investigation revealed that the parent PB 69 (plant height and yield), PB 67 (total number of fruits per plant, number of marketable fruits per plant and yield), SMB 115 (earliness, number of marketable fruits per plant and total number of fruits per plant), PB 66 (weight of marketable fruits per plant, number of marketable fruits per plant, total number of fruits per plant and yield), PB 64 (plant height, number of primary branches and fruit length), KS 331 (earliness, number of primary branches, plant height, fruit length), BARI (fruit length and earliness), Pant Rituraj (earliness and yield), Pant Samrat (total number of fruits per plant), PB 70 (earliness, total number of fruits per plant and yield), Pusa Upkar (earliness, plant height, weight of marketable fruits per plant and yield) were promising donor based on general combining ability. The cross BARI × PS showed highest economic heterosis and sca effects for most of the traits studied including the yield and yield attributing characters. Thus, this cross could be advanced to recover desirable segregants for the improvement of yield and yield contributing characters. The fourteen genotypes used as parents in present study could be distinguished based on sodium dodecyl sulphate polyacrylamide gel electrophoresis of seed proteins. They were distinguished into nine groups on different banding patterns in three zones (A, B and C). The UPGMA analysis showed that PB 60, PB 68 PB 64, Pant Rituraj, PB 66, BARI and PB 69, KS 331, PB 70, SMB 115, Punjab Sadabahar, Pant Samrat, Pusa Upkar formed two different clusters. However, PB 60; PB 68; PB 64 and PB 66; BARI and Pant Rituraj; KS 331, PB 69 and Punjab Sadabahar; PB 70, SMB 115 and PB 70; Pusa Upkar; and Pant Samrat were three different neighboring groups.ThesisItem Open Access Genetic analysis of yield components and molecular characterization in two botanical varieties of Cucumis sativus l. vars. sativus and hardwickii(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-02) Choudhary, Harshawardhan; Singh, Dinesh KumarThe present investigation was carried out at Vegetable Research Centre during 2006-2008 and molecular characterization was performed in the molecular marker laboratory, Department of Plant Pathology, G.B. Pant University of Agriculture and Technology, Pantnagar. The major objectives of this study were to know variability parameters, heritability, genetic advance, genotypic and phenotypic correlation in Cucumis sativus var. hardwickii and study of heterosis, combining ability and generation mean analysis in crosses between cucumber genotypes and germplasm lines of Cucumis sativus var. hardwickii along with molecular characterization through RAPD marker. The salient findings are summarized as follows. Analysis of variance revealed highly significant differences among the genotypes for 15 characters which clearly indicated that there was enough variability for these traits among the germplasm lines utilized for the present investigation. Most of the germplasm lines of Cucumis sativus var. hardwickii were late in flowering. These lines had high number of lateral branches per plant (6.44) and number of fruits per plant (18.57) but fruit length (5.03cm) , fruit diameter (3.84cm) and average fruit weight (53.37g) were low. Fruit yield per plant ranged from 316.83g (IC 277048) to 1563.95g (IC 331620) and its mean value was recorded as 707.22g. Highly significant and positive phenotypic correlation was found between fruit yield per plant and number of fruits per plant (0.76), average fruit weight (0.73), pulp breadth (0.73), fruit length (0.71) and fruit diameter (0.68). Only one cross PCUC 15 × P7 showed superiority over better parent (PCUC 15) in negative direction for days to female flower. Seven crosses exhibited significant heterosis over standard parent in positive direction for no of lateral branches per plant. Three crosses showing significant heterobeltiosis for number of fruits per plant were PCUC 8 × P7 (44.22%), PCUC 28 × P5 (18.61%) and PCUC 15 × P7 (18.44%). Four crosses showed superiority over better parent (cucumber genotypes) for fruit yield per plant. The highest value of heterobeltiosis was estimated in cross PCUC 15 × P3 (61.61%) followed by PCUC 15 × P7 (50.90%), PCUC 8 × P7 (43.01%) and PCUC 15 × P5 (28.43%). The cucumber genotype PCUC 28 was identified as best general combiner for earliness, number of fruits per plant, average fruit weight, fruit length and fruit yield per plant. The genotypes P7, P1 and P3 have been identified as good general combined for number of fruits per plant and fruit yield per plant. The cross PCUC 28 × P1 exhibited high sca effects in desired direction for 8 characters of economic importance. The crosses PCUC 8 × P7 showed high sca effects for 6 characters and PCUC 15 × P3 for 4 characters including number of fruits per plant and fruit yield per plant. Generation mean analysis showed that additive components were predominant for days to female flower anthesis, vine length and average fruit weight. Interaction effects were predominant for number of lateral branches, number of fruits per plant, fruit length and fruit yield per plant. The seventeen genotypes were grouped into 5 different clusters on the basis of RAPD analysis showing enough genetic diversity among them.
