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Subject: Vegetable Science × Author: Bisht, Yashpal Singh × End date: 2024 × Start date: 2020 × Type: Thesis ×
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    ThesisItemOpen Access
    Augmenting heterosis using Pc, F/Acr genes for protected conditions and molecular diversity studies in cucumber (Cucumis sativus L.)
    (G.B. Pant University of Agriculture and Technology, Pantnagar, District Udham Singh Nagar, Uttarakhand. PIN - 263145, 2022-09) Bisht, Yashpal Singh; Singh, Dinesh Kumar
    The present investigation was carried out at Vegetable Research Center of G. B. Pant University of Agriculture and Technology, Pantnagar with the objectives to estimate heterosis for metric and quality traits, GCA and SCA of parents & hybrids, screening for powdery mildew disease and molecular diversity study in Cucumber (Cucumis sativus L.). The analysis of variance for RBD and combining ability revealed the significant genetic differences among 44 cucumber genotypes for the 12 yield contributing traits under study. The magnitudes of PCV estimates were higher than the corresponding GCV estimates for all the characters. Moderate to high GCV together with moderate to high heritability and genetic advance as per cent of mean was reported for majority of the characters under study except days to first female flower, days to first harvest, days to last harvest, vine length and internodal length. Three cross combinations namely PPCUC-3 × PCUC-8, PPCUC-3 × PCUC-28 and PPCUC-11 × PCUC-8 in 2021 and PPCUC-4 × PCUC-8, PPCUC-7 × PCUC-8 and PPCUC-9 × PCUC-28 in 2022 have significant positive heterosis for maximum numbers of traits and showed their value in combining ability studies. For quality traits, PPCUC-10 × PCUC-28 followed by PPCUC-3 × PCUC-28 and PPCUC-12 × PCUC-28 have significant positive heterosis for maximum numbers of traits. Therefore, these cross combinations can be commercially exploited for hybrid development after multi location testing. PPCUC-3 was identified as a best general combiner for maximum number traits followed by PPCUC-9 and PPCUC-10. The best specific combiner were PPCUC-9 × PCUC-51 followed by PPCUC-2 × PCUC-8 in 2021 and PPCUC-10 × PCUC-51 was best specific combiner in 2022 for metric traits. For quality traits, PPCUC-12 × PCUC-28 followed by PPCUC-4 × PCUC-51 was best combiner. Hence, these crosses can be utilized in heterosis breeding for improvement in yield and quality related traits. For powdery mildew disease resistant reaction, out of all parents and F1 hybrids only check variety ‘Poinsett’ exhibited moderately susceptible reaction while none of the parents and hybrids showed resistant reaction to powdery mildew. Susceptible reaction against powdery mildew was observed in three genotypes viz., PPCUC-2 × PCUC-28, PPCUC-3 × PCUC-28 and PPCUC-7 × PCUC-8. Eleven SSR primers were used for the molecular diversity analysis. Out of eleven primers, four primers generated polymorphic bands. Total 15 bands were amplified by 11 SSR primers in the 14 cucumber genotypes of which polymorphic and monomorphic bands were 8 and 7, respectively. The range of amplified products was 100-400 bp approximately. Average number of bands per primer was 2. The PIC values of SSR markers ranged from 0.133 (SSR 11742) to 0.408 (SSR 19493) with mean value of 0.262 in which marker, SSR 11742 was least informative while marker, SSR 19493 was most informative due to highest PIC value among other markers, therefore this type of marker system could be efficiently applied to detect polymorphism in cucumber. Jaccard’s similarity coefficient varied from 0.71 to 1.00. Minimum Jaccard’s similarity coefficient was found among PPCUC-11 with PPCUC-2.