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Theses

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2000 - 200928
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Subject: Seed Science and Technology × End date: 2009 × Start date: 2000 × Type: Thesis ×
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Now showing 1 - 9 of 28
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    ThesisItemOpen Access
    Influence of seed treatment with rhizospheric bacterial isolates on seed vigour, yield and it’s contributing characters in soybean (Glycine max L. Merril)
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-06) Amrendra Kumar; Prasad, Birendra
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    ThesisItemOpen Access
    Studies on seed quality parameters in different populations of finger millet (Eleusine coracana (L) Gaertn.)
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-07) Ankita; Prasad, Rajendra
    The present investigation was conducted during Kharif 2008, at Hill Campus, Ranichauri with three populations of finger millet with view to study the field and seed quality parameter in brown, white and amber populations. The field experiment was laid out in randomized complete block design. The observation recorded for morphological and yield contributing characters in field while seed quality assessment experiment was conducted in laboratory in completely randomized design. The analysis of variance revealed significant variation among the lines of different brown, white and amber populations. Amber population took minimum time for flowering and maturities, similarly maximum number of finger per ear were also observed in amber population. Maximum length of ear, finger length and finger width were observed in white population. Maximum score for neck and finger blast was recorded in white population while amber population observed least infestation. For most of the seed quality parameters white population found superior than brown and amber population. Maximum variability estimates were recorded for seed yield per plant and first count. Most of the field and seed quality parameters recorded high estimates for broad sense heritability. All of the seed quality parameters exhibited positive genotypic and phenotypic correlation with each other. Based on the studies it was concluded that all three populations included in the present study having wide array of variation for different yield and yield contributing traits and seed quality parameters.
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    ThesisItemOpen Access
    Effect of moisture stress on germination and seedling growth of vegetable pea (Pisum sativum L.)
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-01) Prasad, Shambhoo; Shukla, Prabha Shankar
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    ThesisItemOpen Access
    Effect of moisture stress on germination and seedling growth of vegetable pea (Pisum sativum L.)
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-01) Shambhoo Prasad; Shukla, Prabha Shankar
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    ThesisItemOpen Access
    Seed quality parameters of rice bean (Vigna umballata) and adzuki bean (V. angularis) and associated seedborne mycoflora in mid hills of Uttarakhand
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2007-07) Upreti, Kumud; Khulbe, Deepa
    The present investigation was carried out under field and laboratory conditions to assess different rice bean and adzuki bean entries on the basis of seed quality parameters and associated seed borne mycoflora. The results revealed highly significant variations based on seed morphological parameters in both the crops. Seed length, breadth and weight were positively and non-significantly correlated with fresh weight and seed yield. All these three parameters were positively and highly significantly correlated with dry weight of seedlings in case of adzuki bean. Rice bean and adzuki bean lines were divided into three colour categories viz. light green, light brownish green and shiny black; and maroon, yellowish cream and dark gray with black patches, respectively. Seed weight was positively correlated to seedling vigour II in all the rice bean accessions. Root length, shoot length were positively, but non-significantly correlated with seed yield, however, were positively and highly significantly correlated with seedling vigour I. Root hair density variations showed negative and significantly correlation with shoot and root length, seed length and weight and germination per cent in both the crops. Seedling vigour indices were positively correlated with seed yield. All the lines showed cent per cent seed viability as indicated by positive reaction to tetrazolium test. Shoot length variation of adzuki bean accessions ranged from 9.67 to 22.0 cm in EC 8707 and SMLAB1, respectively. Seedling length was recorded highest in EC 30253 (36.07 cm) and least in EC 8707(22.33 cm) and hierarchical cluster analysis resulted in 3 major groups with 6 clusters. The fresh weight varied significantly from 0.447 g (EC-349264) to 1.203 g (SMLAB-8). Dry weight variations were highly significant and the accessions could be grouped into 6 clusters. Seedling moisture content of adzuki bean lines ranged from 82.3 (EC-30270) to 92.5 (IC-241041). Seedling vigour index I ranged from 2032.3 to 3606.7 in EC-8707 and EC-30253, respectively. Seedling vigour index II value ranged from 7.25 (EC-30270) to 18.99 (EC-340247) and the accessions could be categorized into 3 major groups with 6 clusters, Seedling index II was positively, but, non-significantly correlated with seed yield. Out of different methods used, blotter test brought out maximum number of seed associated fungi. The mycoflora isolated from different accessions of rice bean and adzuki bean were Alternaria alternata, Helminthosporium sp., Chaetomium globosum, Phoma sp, Rhizoctonia solani, Fusarium sp., Trichoderma, Rhizopus sp, Penicillium, Colletotrichum gloeosporioides. All rice bean entries showed positive peroxidase reaction. Eight adzuki bean lines showed positive reaction to peroxidase activity in seed coat while, 14 accessions were negative. All the accessions showing positive reaction showed disease incidence in the range of 2 and 3 grades, however no definite correlation could be derived. Field emergence ranged from 92.1% (LRB 35-1) to 97.6% (PRR 1) and diseased seedling per cent ranged from 2.35% to 7.88% in PRR 1 and LRB 35-1, respectively, in rice bean entries. Ten rice bean accessions were found to be resistant to Phoma leaf blight while, four were moderately resistant. Seed yield was highest (18.66 q/ha) in PRR 2 and lowest (5.08 q/ha) in RBL 1. In case of adzuki bean, field emergence of SMLAB 8 was highest (97.4%) while, it was lowest (91.4%) in EC 000254. Diseased seedling per cent varied from 2.6 to 8.6 in SMLAB 8 and EC 000254, respectively. No line was found to be resistant to Phoma leaf blight, however, ten lines showed moderately resistant reaction.
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    ThesisItemOpen Access
    Studies on seed quality parameters in different generations of finger millet (Eleusine coracana (L) Gaertn.)
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2007-07) Kalyanrao; Rajendra Prasad
    The present investigation was conducted during Kharif 2006, at Hill Campus, Ranichauri with seven generation and two parents. The experiment was laid out in randomized block design with three replication. Characters were studied for variability viz. shape of ear head, seed colour, plant height (at 30 days, 60 days and maturity), flag leaf length, flag leaf width, number of leaves per plant, finger length, finger width, number of grain per finger, number of tillers per plant, number of ear bearing tillers per plant, days to 50% flowering, days to maturity and seed yield per plant. seed quality parameters viz. 1000 seed weight, root length, shoot length, fresh weight of seedling, dry weight of seedling, seedling moisture content, viability percentage, germination per cent, vigour index I and vigour index II. Character association was also computed for field and seed quality parameters. Significant differences in quantitative characters were observed. The plant height at 30 days ranged from 41.77 cm (F6 generation) to 53.70 cm (Gaja local), plant height at 60 days ranged from 81.03cm (F7 generation) to 109.2cm (Gaja local), plant height at maturity 114.6cm (F7 generation) to 123.4cm (INFM 95001), days to 50% flowering 103.0 days (F7 generation) to 125.6 days (INFM 95001), finger length 5.10cm (F7 generation) to 9.16cm (Gaja local), finger width ranged from 0.866cm (F6 generation) to 1.733 (F1 generation), number of leaves 9.66 (F5 generation) to 14.00 (INFM 95001), number of tillers per plant ranged from 1.0 (F7 generation) to 4.00 (INFM 95001), number of ear bearing tillers per plant 1.00 (F7 generation) to 3.00 (INFM 95001), number of grain per finger ranged from 34.67 (INFM 95001) to 300.70 (F1 generation), days to maturity 143.7 (F7 generation) to 178.0 (INFM 95001), seed yield per ear head 1.182 g (INFM 95001) to 7.473 g (F1 generation). 1000 seed weight ranged from 2.684 (F8 generation) to 4.123(INFM 95001), germination % varies from 88.33 % (F4 generation) to 96.67 % (F8 generation), seedling fresh weight 0.112 (F4 generation) to 0.172 (INFM 95001), seedling dry weight 0.0080 (F4 generation) to 0.0163 (F3 generation), viability percent ranged from 91.00% (INFM 95001) to 98.43 (F8 generation), seedling moisture content varies from 84.33% (F3 generation) to 94.35% (F7 generation), vigour index I ranged from 1084.0 (F4 generation) to 1194.0 (F8 generation), vigour index II 0.707 (F4 generation) to 1.147 (F3 generation).
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    ThesisItemOpen Access
    Studies on quality parameters and effect of storage on seeds of proso millet (Panicum miliaceum L)
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2006-07) Jain, Atul Kumar; Rajendra Prasad
    The present investigation was conducted during Kharif 2005, at Hill Campus, Ranichauri with thirteen accessions. The experiment was laid out in randomized block design with three replication. Characters were studied for variability viz. shape of inflorescence, seed colour, plant pigmentation at flowering, blade pubescence, plant height, flag leaf length, flag leaf width, peduncle length, inflorescence length, days to 50% flowering, days to maturity, seed yield per plant and chlorophyll content. seed quality parameters viz. 1000 seed weight, root length, shoot length, fresh weight of seedling, germination per cent, speed of germination, vigour index I, vigour index II and electrical conductivity. Seed quality parameters were also evaluated for seed taken from different portion of panicle and for three year old and fresh seed sample. Character association was also computed for field and seed quality parameters. Significant differences in quantitative characters were observed. The plant height ranged from 74.00 cm (GPMS-877) to 121.00 cm (GPMS-596), flag leaf length 24.00 cm (GPUP-8) to 47.06 cm (GPMS-596), peduncle length 2.40 cm (GPMS-877) to 13.53 (GPMS-592), days to 50% flowering 54 days (GPMS-862) to 67 days (GPMS-593), days to maturity 105 days (GPMS-862, 877) to 124 days (GPMS-595), inflorescence length 15.56 cm (GPMS-877) to 29.06 cm (GPMS-591), seed yield per plant 4.05 g (GPUP-8) to 12.04 g (GPMS-595-1). Speed of germination ranged from 12.33 (GPMS-591) to 26.41 (GPMS-598), germination % 39.00 % ( GPMS-597) to 97.33 % (GPMS-595-1), root length 4.61 cm (GPMS-877) to 9.21 cm (GPMS-595-1), shoot length 2.50 cm (GPMS 592-1) to 6.41 cm (GPMS-595-1), 1000 seed weight 4.60 g (GPMS-594) to 5.901 g (GPMS-8), vigour index I 408.70 (GPMS-597) to 1020 (GPMS-595-1), vigour index II 0.529 (GPMS-597) to 1.163 (GPMS -598). Total chlorophyll ranged from 0.661 mg/g (GPUP-8) to 2.10 mg/g (GPMS-594). Comparative study for seed taken from different portion of panicle showed upper portion performed better than middle and lower. In comparison between old and fresh seed, old seeds performed better than fresh, however some genotypes of fresh sample such as GPMS-595-1, GPMS-596, GPMS-595, GPMS-552, GPMS-877 showed similar or higher performance with old sample.
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    ThesisItemOpen Access
    Characterization of ricebean (Vigna umbellata (Thunb.) Ohwi & Ohashi) varieties and their narrow leaf crosses using morphological, biochemical and molecular techniques
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2006-06) Islam, Md. Shafiqul; Dutta, M.
    The present investigation was carried out under field and laboratory conditions to characterize thirteen ricebean varieties and their eleven crosses using morphological and physiological parameters, chemical tests, seed vigour parameters, biochemical test (soluble seed protein content) and molecular technique (RAPD analysis). The results revealed that the seed morphological parameters of ricebeaqn varieties and their crosses, viz. seed coat colour and test weight in combination with morphological parameters of adult plant, viz. days to flowering, leaf shape, leaf length and breadth, plant height, number of primary branches per plant, petiole length, number of seeds per pod, etc. better classified ricebean varieties and their crosses into distinct groups. Seed coat peroxidase activity test, phenol colour reaction test, NaOH bleach test and KOH bleach test were used in the present study. Among these seed coat peroxidase activity test was found to be effective in characterization of ricebean varieties and their crosses. Biochemical parameter, viz. soluble seed protein content was also effective in characterization of ricebean varieties and their crosses. A RAPD-PCR protocol was standardized in the present study. For a total level of seventeen primers, 147 marker levels were amplified of which 91 (61.9%) were polymorphic bands across the genotypes. The present study revealed that the RAPD technique with the set of seventeen primers was sufficient to identify the uniqueness of twenty four ricebean varieties and their crosses. Per cent polymorphism among twenty four ricebean varieties and their crosses ranged from 38.9 per cent to 59.8 per cent. On the other hand, percent polymorphism among eleven crosses ranged from 40.5% to 53.0%. The results illustrated that the ricebean varieties and their crosses manifested moderate level of polymorphism. This indicated that among the varieties and crosses moderate level of diversity was present as they originated from different places within the country. Cluster analysis based on UPGMA (Unweighted Pair-Group Method for Arithmetic Average) analysis with Squared Euclidean Distance revealed the patterns of relatedness among the ricebean varieties and their crosses. It was observed that when cut off value was considered at a linkage distance of 40, the ricebean varieties and their crosses were grouped into nine clusters. The cluster analysis showed considerable amount of genetic diversity among the ricebean varieties and their crosses. The polymorphism observed between the varieties and crosses were used as markers for hybrid analysis. The patterns of RAPD markers were classified into seven types according to the presence or absence of bands. The present investigation indicated that out of the seventeen RAPD primers used most of them were useful with good amount of reliability to identify ricebean crosses showing 49.5% to 68.0% shared markers with the parents (Type I, III and IV). However, Type VII markers were useful in identifying new cultivars of ricebean with 5% to 17.4% non-parental bands. The present investigation demonstrated that RAPD molecular marker analysis could be quite useful in characterization of ricebean varieties and their crosses as well as the genuineness of parents and their crosses could be effectively determined.
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    ThesisItemOpen Access
    Morphologigal and biochemical characterization of buckwheat (Fagopyrum spp.) genotypes
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2005-07) Mohd. Naseem; Dutta, M.
    The present investigation was carried out under field and laboratory conditions to characterize twelve buckwheat genotypes consisting of two Esculentum and ten Tutaricum types using seed morphological, plant morphological and physiological characters; chemical tests; seed vigour parameters and biochemical (SDS-PAGE) analysis of buckwheat seed proteins. The results revealed that the morphological parameters of buckwheat seed viz., seed coat colour and seed shape in combination with morphological parameters of adult plant viz., species type, days to flowering, leaf shape, petiole colour, plant height, etc. and physiological characters such as chlorophyll content, transpiration, photosynthesis, etc. better classified the genotypes into distinct groups. The chemical parameters like phenol colour reaction, NaOH and KOH bleach test can be used effectively in order to characterize and develop identification key for buckwheat genotypes. However, seed coat peroxidase activity test was not found to be effective in identification of buckwheat genotypes. It was also found that the seed vigour parameters alone viz., germination percentage, viability, vigour index I and II etc. were of minor importance in characterizing the buckwheat genotypes, as varieties could be classified into few distinct groups only. However, when these parameters were applied in combination with other characters, a better classification was obtained. Biochemical parameters viz., SDS-PAGE protein profile of soluble seed proteins was used effectively for further characterization of buckwheat genotypes unambiguously which could be used as genetic marker as well. The result of the study indicated that to get an effective genotypic classification of buckwheat, a judicious combination of parameters should be used rather than a single set of characters alone.