Thumbnail Image

Theses

Browse

20041
Reset filters
Subject: Poultry Science × Author: Gammi, Taking × End date: 2004 × Type: Thesis ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    Prophylaxis against salmonellosis through Salmonella toxoid vaccine and competitive exclusion
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2004-08) Gammi, Taking; Ashok Kumar
    The present investigation was conducted to evaluate the immunoprophylaxis efficacy of Salmonella toxoid vaccine by the various routes of administration- subcutaneously and orally to individual chick and orally through drinking water to chicks ageing 21 days by dividing into four groups. The blood sera were assessed upto 90th DPV by ELISA test, also challenge test were conducted by dividing the four groups into eight subgroups and challenged with S. Weltevreden and S.Gallinarum @ 4 x 109 c.f.u. The clinical symptoms with mortality pattern were studied upto 30th DPC and the liver, caeca and spleen samples were collected from the birds died of challenge infection and the sacrificed survivor for bacteriological examination. The maximum humoral antibody titres were obtained from the subcutaneously vaccinated birds and the 90-100% protection were afforded by the vaccine. The mortality pattern of vaccinated and control birds were also studied and compared. The passive transfer of maternal antibodies were assessed by detecting the saline extraction of antibodies in the egg yolk which obtained a high antibody titres. Also protective efficacy of maternal antibodies were evaluated by the challenge test which obtained a 90-100% protection. The cross-protection against E.coli was evaluated by challenging the four groups of birds by the commonly occurring E.coli serovars 0-78 @ 4 x 109 c.f.u intraperitoneally on 21st DPC, which obtained a 83.33% protection. The protective efficacy of one year old vaccine were evaluated by ELISA test, AGPT and challenge test. The equal antibody titres of freshly prepared vaccine were obtained from the sera collected on 21st DPV and precipitation line was obtained in between the serum of vaccinated birds and antigen, also 90-100% protection were afforded by the vaccine. The prophylaxis against salmonellosis by the competitive exclusion (probiotic) was studied and obtained a improvement of growth rate, gained in body weight by the probiotic feed chicks of day old upto a Av. Wt. gain of 5gm/chick. Also, gave cross-immunity and powerful degree of competitive exclusion between two or more serovar were obtained.