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Subject: Plant Pathology × Author: Singh, Omkar × End date: 2021 × Start date: 2021 × Type: Thesis ×
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    ThesisItemOpen Access
    Development of Iron–biofortified button mushroom (Agaricus bisporus) using nanoparticle
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-08) Singh, Omkar; Kushwaha, K.P.S.
    Nutrient deficiency is one of the major global health problem, with iron deficiency being a leading cause of many ailments. Amongst several approaches that have been tried to combat this problem, use of bio-fortified foods is one of the most promising one. It not only increases the intake of nutrient but also augments its bioavailability. Mushrooms have been a source of good nutrition since ancient times; among which Agaricus bisporus is a widely consumed species. Due to its high nutritional value, but relatively low iron content, an attempt was made in the present investigations to developed iron bio-fortified Agaricus bisporus, using iron oxide and iron sulfide nanoparticles. Different concentrations of iron nanoparticles (0, 3, 6, 9, 12 and 15 ppm) were used to supplement the mushroom at three different stages i.e at spawning stage, before casing stage and pin head initiation stage. Treated mushroom was analyzed for various attributes viz. mycelial growth, crop yield, iron content, sensory evaluation and antioxidant activity. Highest mycelial growth, crop yield and iron content were obtained in 9ppm in case of iron oxide and at 12 ppm in case of iron sulfide nanoparticle treated Agaricus bisporus. The antioxidant status measured in terms of flavonoid and phenol content was also recorded to be highest in 9ppm in and 12 ppm treated samples respectively. Phenol content increased with increasing concentration of iron nanoparticles, with 15ppm having maximal content. Proline and hydrogen peroxide content decreased continuously from 0 to 15 ppm. Thus from the above investigations it can be concluded that use of nano-biotechnology could be an efficient way to bio-fortify Agaricus bisporus along with enhanced iron and antioxidant activity.