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Subject: Molecular Biology and Biotechnology × End date: 2017 × Start date: 2010 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Marker assisted conversion of normal maize into quality protein maize
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2014-01) Tufchi, Mahak; Singh, N.K.
    The present investigation on “Marker assisted conversion of normal maize into Quality Protein Maize” was carried out with the objective to convert normal maize lines into quality protein maize (QPM). Polymorphisms among normal maize lines namely Pant 10k 1375, Pant 11R-53, Pant 11R-126 and Pant 11R-128 and QPM donor lines namely CML161, CML162, CML187 and CML189 were determined using both molecular and phenotypic markers. The polymorphic parents were crossed to generate F1s. Nine selected F1s were used to generate BC1F1s and BC2F1s populations by subsequent crossing with their respective recurrent parents. Individual plants from BC1F1s and BC2F1s populations heterozygous for o2 allele were selected using SSR marker phi057 whereas phenotypic criteria in both generations followed by marker assisted selection of loci other than o2 locus in BC2F1s were used to select plants similar to recurrent parents. The number of plants selected in BC1F1s and BC2F1s populations varied from 12 to 24 and from 10 to 28, respectively. All the BC2F2 individuals were screened using light box and plants with kernels less than 25% opaqueness were selected which varied from 2 plants in Pant11R-128xCML189 to 10 plants in Pant11R-126xCML162. Selected progenies were advanced to BC2F3 by self pollination and self seeds of each plants were used to determine protein, tryptophan, lysine, quality index (QI) and carotenoids. The number of lines across the nine population with tryptophan higher than 0.075% were 29 whereas the number of lines identified to be QPM based on protein, tryptophan and QI were 5, 3, 2, 2, 1, 1, 2, 2, 1 in Pant10k1375xCML161, Pant10k1375xCML187, Pant11R128xCML187, Pant11R53x CML189, Pant11R128xCML189, Pant11R126xCML189, Pant11R53xCML162, Pant11R126xCML162 and Pant11R128xCML162, respectively. These QPM lines could be a potential source of germplasm in development of single cross QPM hybrids in different combination among themselves as well as in combination with other diverse QPM lines. Analysis of carotenoids revealed that four lines namely BC2F3:17, BC2F3:8, BC2F3:10, BC2F3:14 derived from the cross between Pant10k1375xCML161 had carotenoids higher than the higher parent and therefore indicates that carotenoids can be enhanced using classical breeding approach. The relation between kernel colour and carotenoids content was found to be significant positive. However, regression analysis revealed low coefficient of determination of kernel colour on carotenoids as indicated by R2 value of 0.204. Pair-wise correlation between protein, tryptophan, lysine, QI and carotenoids were positive however low coefficient of determination indicated that one parameter cannot be used in determination of others