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2003 - 20042
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Subject: Microbiology × End date: 2004 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Genetic characterization of root nodule isolates from clones of Dalbergia sissoo
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2003-08) Samant, Mukesh; Johri, B.N.
    Dalbergia sissoo (Shisham) is an important Leguminous nitrogen fixing tree of tropics. Legume-Rhizobium symbiosis is important for nitrogen fixation. Therefore, present investigation was undertaken to study the genetic diversity of rhizobial partner of Dalbergia sissoo based on biochemical and molecular tools e.g., 16 S ARDRA, IGS ARDRA, BOX PCR profiling and Nif H amplification. ARDRA of 16 S rDNA region of isolates from clones of Dalbergia sissoo (CPT 5 and CPT 6) resulted in placement of all six isolates in separate group from other authentic rhizobial and Shisham isolates. IGS ARDRA showed higher diversity within these isolates than 16 S ARDRA. UPGMA cluster analysis of combined IGS and 16 S ARDRA showed diversity within the isolates of CTP 5 (DS 5 1a and DS 5 1b) and CPT 6 (DS 6 1a, DS 6 1b, DS 6 1c and DS 6 1d) but they clustered in the same group. None of the isolate was identical to other authentic rhizobial strain and other Shisham isolates. BOX PCR profile showed some identical band pattern in all the isolates. All isolates were Nif H positive suggesting operation of nitrogenase. DNA sequencing of these isolates will permit taxonomic identification and further phylogenetic assignment.
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    ThesisItemOpen Access
    Screening of isolates of Saccharomyces sp for bioethanol production from damaged chiku (Achras zapota) fruits
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2004-08) Neelam; Tewari, Lakshmi
    Looking towards need for development of an alternative renewable source of energy due to depleting petroleum resources, attempts were made to utilize damaged/inferior quality sapota (Achras zapota) fruits for production of bio-ethanol by microbial fermentation. Different strains of yeast (Saccharomyces sp.) were isolated from different damaged sources viz. fruit surfaces (pear, apple), pulp (banana), sugarcane juice, curd and bakery-dough. The isolates were characterized using morphological, biochemical and physiological markers and compared with an standard culture of Saccharomyces cerevisiae ATCC 9763, MTCC 170 (CDRI). All the yeast cultures were analyzed for their colonial and cellular characteristics; growth rate and pattern in liquid and solid media were measured. The isolates were analyzed for their in vitro fermentation and utilization of different sugars, in vitro alcohol tolerance. Different sugars in sapota juice were identified by TLC. The isolates were evaluated for their alcohol producing capacity by liquid state and submerged fermentation of sapota fruits. The potential strains having high alcohol tolerance and alcohol production were selected for alcoholic fermentation of sapota fruits.