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Screening of isolates of Saccharomyces sp for bioethanol production from damaged chiku (Achras zapota) fruits
(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2004-08) Neelam; Tewari, Lakshmi
Looking towards need for development of an alternative renewable source of energy due to depleting petroleum resources, attempts were made to utilize damaged/inferior quality sapota (Achras zapota) fruits for production of bio-ethanol by microbial fermentation. Different strains of yeast (Saccharomyces sp.) were isolated from different damaged sources viz. fruit surfaces (pear, apple), pulp (banana), sugarcane juice, curd and bakery-dough. The isolates were characterized using morphological, biochemical and physiological markers and compared with an standard culture of Saccharomyces cerevisiae ATCC 9763, MTCC 170 (CDRI). All the yeast cultures were analyzed for their colonial and cellular characteristics; growth rate and pattern in liquid and solid media were measured. The isolates were analyzed for their in vitro fermentation and utilization of different sugars, in vitro alcohol tolerance. Different sugars in sapota juice were identified by TLC. The isolates were evaluated for their alcohol producing capacity by liquid state and submerged fermentation of sapota fruits. The potential strains having high alcohol tolerance and alcohol production were selected for alcoholic fermentation of sapota fruits.