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Subject: Microbiology × Author: Singh, Digar × End date: 2009 × Start date: 2007 ×
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    ThesisItemOpen Access
    Molecular characterization of the Acetobacter diazotrophicus species, isolated from native sugarcane varieties of hilly areas of Uttarakhand
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-07) Singh, Digar; Sharma, Anita
    Seven endophytic bacterial isolates were recovered from native sugarcane varities from hilly areas namely Berinag, Champawat and Didihat of Uttarakhand state. All the isolates along with two standard cultures – Azospirillum brasilense and Gluconoacetobacter diazotrophicus , were tested for their morphological , biochemical and molecular characterizations .All the isolates were found gram negative, rod shaped showing morphological similarity with the standard cultures. Their PGPR properties were also assessed, Bacterial isolates, Rt.Bn. and St.Bn. were IAA producing. Except St.Bn, all the isolates solubilized insoluble phosphate and produced Siderophore except Rt.Bn. Molecular characterization of the isolates was done by ARDRA profiling using four restriction enzymes, Alu I, Msp I, Hae III and Mnl I. Cluster analysis using UPGMA programme grouped the isolates as per their similarity of geographical distribution, showing insignificant similarity with the standards used. Multiple bands of nif H amplicon were observed in all the isolates (except RtChI and StCh) indicating their nitrogen fixing character.