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20191
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Subject: Microbiology × Author: Agri, Upasana × Type: Thesis ×
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    ThesisItemOpen Access
    Combined effect of nanochitosan and potential bioinoculant on maize plant and its rhizosphere
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2019-07) Agri, Upasana; Sharma, Anita
    Present study focuses on the application of agriusable nanochitosan along with potential plant growth promoting rhizobacteria on maize plants under pot experiment. PC1 and PC4 were selected on the basis of their plant growth promoting properties and identified as Pseudomonas taiwanensis and Pantoea agglomerans with accession number MK106029 and MK106024 respectively. They were found compatible with each other and along with nanochitosan (40ppm) which was further supported by SEM results showing no inhibitory effect of Nch on bacterial cultures. Percent seed germination was significantly higher upto 98% in the combined treatment of nanochitosan and PGPR under pot experiment. Application of Nch and bioinoculants enhanced plant growth parameters. A significant increase (>1.5 fold) in carotenoid, total sugar, total phenol was observed in seedlings treated with nanochitosan and bacterial cultures after 30 days in comparison to control. Total catalase and peroxidase activity was found maximum in PC1+PC4+Nch treatment which accounted for 16.25 and 43.67 μmol/min/mg protein after 30 days of pot trial. Enhancement in total fluorescein diacetate and alkaline phosphatases enzyme activity was observed in PC1+PC4+Nch treated soil with (>1.6 fold increase) in comparison to control. Low level of dehydrogenase activity was found in Nch treated soils. physiochemical properties like oxidizable organic carbon, available phosphorus, and potassium and ammonical nitrogen were higher in Nch treated soil as compared to control. Improvement in soil health was observed in all treatments after 30 days in comparison to control. 1.5- 2 fold increase in total microbial population was observed in soil treated with Nanochitosan and bacterial cultures. Population based metagenomics analysis revealed no negative impact of nanochitosan on microbial population in total. It can be concluded from our findings that application of nanocompound along with bacterial treatments improved plantbacterial interaction, promoted plant parameters, increased microbial diversity and increased the level of soil health indicator enzymes.