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Theses

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2016 - 20192
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Subject: Livestock Production and Management × Author: Dar, Aashaq Hussain × End date: 2019 × Start date: 2010 ×
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    ThesisItemOpen Access
    Evaluation of phenotypic and genetic diversity in different colour variants of Badri cattle using neutral and functional markers
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2019-07) Dar, Aashaq Hussain; Sanjay Kumar
    The present study was conducted on Badri cattle and its three colour variants viz., grey, brown and black from Uttarakhand state of India. Efforts were made for phenotypic and genotypic characterization of Badri cattle. For genetic characterization, microsatellite (SSR) and gene based markers were utilized to assess the general and functional genetic diversity in Badri cattle. Sampling was done from both Kumaon and Garhwal divisions of Uttarakhand, covering five hilly districts from each divisions. For genetic characterization, a total of 96 blood samples collected; 90 samples from the breeding tract (9 samples each from the 10 districts) and 3 samples each from University Dairy Farm, GBPUAT, Pantnagar and State Breeding Farm, Champawat . Phenotypic characterization of Badri cattle indiacted that it is a small sized cattle with an average adult body weight of about 200-250 kg. The body is compact and cylindrical with tight skin. The muzzle, horn, eyelids, switch of tail and hooves are mostly black in colour. Udder is small sized and trough (bowl) type. The fore and hind quarters of udder were small and medium sized respectively. The teat shape was cylindrical (70 and 78 %) and funnel type (30 and 32%) in Kumaon and Garhwal respectively. Data analysis revealed that breeding tract regions had no effect while as sex and age has significant effect on morphometric traits of Badri cattle. Badri cattle produce about 1.61 ±0.02 kg milk per day of lactation. The average lactation length, dry period, age of puberty, age at first calving, service period and calving interval were found to be 323.10 ±2.71, 82.20 ±0.99 days, 3.38 ±0.05 years and 4.38 ±0.05 years and 5.12 ±0.09 and 14.05 ±0.09 months respectively. The milk constituents viz. fat, protein, lactose, SNF and total solid percentage was higher than exotic cattle and some Indigenous breeds. The present study revealed that the mineral composition of Badri cattle milk is comparable with other cattle breeds. Ca, Mg, P, and Na content increased significantly in the third stage of lactation while potassium showed a reverse trend. The average serum glucose, total protein, triglycerides, cholesterol, creatinine and urea were found to differ significantly in summer and winter seasons. Using light microscopy, it was revealed that the hair shaft and medullary diameter at medial region and both regions of body i.e. dorsal and ventral, differed significantly within colour variants of Badri cattle, i.e. Grey, black and brown variants. At both dorsal region and ventral region within the colour variant groups, there was a significant difference between cortical thickness of hair shaft at proximal, medial and distal part. Scanning electron microscopy (SEM) revealed that the medullary diameter was significantly higher in medial region of hair shaft as compared to proximal and distal part in all the colour variants at both dorsal and ventral regions of the body. The genotypic characterization revealed that there was ample of genetic diversity in all the colour variants. Bottle neck analysis revealed that Badri cattle and its colour variants are under bottleneck. Structure analysis revealed that all the three colour variants of Badri cattle are actually single breed. Further it showed Badri cattle is a distinct breed as revealed by structure analysis with Red sindhi, Sahiwal, Jersey and Holstein Fresian. Individual assignment using GENECLASS software revealed that the individuals in three colour variants were assigned to all the 3 colour groups while a few individuals were assigned to other breeds at a negligible probability. The polymorphism at kappa-casein, beta-casein, alphalactalbumen, beta-globulin, butyrophilin, growth hormone and prolactin were in close range with other Indigenous cattle breeds.
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    ThesisItemOpen Access
    Effect of feeding probiotic , prebiotic and synbiotic as feed additives on the performance of crossbreds
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2016-06) Dar, Aashaq Hussain; Singh, S.K.