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ThesisItem Open Access Genetic and physiological characterization and validation of linked SSR markers for drought tolerance in wheat (Triticum aestivum L.)(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-09) Arun Kumar; Prasad, RajendraWheat is one of the important food crops cultivated in different environmental conditions in India. The present investigation was conducted during 2014-15 and 2015-16 to assess the 160 wheat genotypes for drought tolerance at Norman E. Borlaug Crop Research Centre of GBPUAT, Pantnagar with objective to find out drought tolerant and susceptible genotypes. Five morphological traits namely; leaf morphology, leaf angle, leaf rolling, waxiness on leaf, spike fertility; seventeen genetic traits namely; days to heading, days to anthesis, Days to maturity, Grain filling duration, No. of tillers per plant, Flag leaf length, Flag leaf width, Flag leaf area, Plant height, Peduncle length, Peduncle weight, Spike length, No. of spikelets per spike, No. of grains per spike, Grain weight per spike, 1000-grain weight, Grain yield per plant and five physiological traits namely; Canopy temperature depression, Normalized difference vegetation index, Flag leaf chlorophyll content, Relative water content percent, Flag leaf senescence percent. Analysis of variance for genetic traits under rainfed condition showed that the mean square of the replications had highly significantly differences for all the traits in both years and pooled data, except No. of tillers per plants, spike length were significant only. Mean square of the treatments had highly significantly differences for all the traits in both years and pooled data, except flag leaf width, flag leaf area were significant only. The estimates of correlation coefficients analysis under rainfed condition showed that the grain yield per plant exhibited highly significant positive correlation with 1000-grains weight, grain weight per spike, No. of grains per spike, plant height, grain filling duration, No. of tillers of per plant, peduncle length. The direct and indirect effects of different genetic traits on grain yield per plant under rainfed condition showed that highest positive direct effect on grain yield per plant was exerted by days to maturity followed by flag leaf area, No. of grains per spike, 1000-grain weight, No. of tillers per plants, plant height. Grain weight per spike, peduncle weight, No. of grains per spike, peduncle length exerted substantial positive indirect effects on grain yield per plant via 1000-grains weight. The clustering pattern for genetic traits under rainfed condition grouped into thirteen clusters, the highest number of genotypes appeared in cluster III followed by cluster IV, cluster II, cluster I and cluster XIII. Cluster V, cluster VI, cluster VII, cluster VIII, cluster IX, cluster X, cluster XI and cluster XII with single genotype. Based on markers scores and grain yield per plant, twenty one tolerant genotypes namely; DBW 39, FLW 13, FLW 7, HD 2833, HD 3093, HI 1500, HI 617, HW 2004, HW 2005, HW 2066, HW 4002, HW 4008, HW 4029, HW 4215, Lok-1, Lok-65, MACS 2496, RAJ 4037, SOKOLL, SSRT 14, VJ 99 were selected and eleven susceptible genotypes using same criteria namely; DBW 14, DBW 28, DBW 88, HD 2824, HD 2877, NW 1014, PBW 343, PBW 373, RAJ 4083, UP 2828, MACS 6272 were identified. Identified tolerant and susceptible genotypes can be used for development of mapping population. Identified tolerant genotypes can be recommended for cultivation in rainfed areas.