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  • ThesisItemOpen Access
    Fish landing patterns and gonadal cyclicity of Notopterus notopterus (Pallas) in Baigul reservoir of Uttarakhand
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-05) Mondal, Anandamoy; Singh, I.J.
    The present study was undertaken to investigate fish landing and the reproductive cycle of the fish Notopterus notopterus in the Baigul reservoir located in the Uttarakhand state during the year 2006-07. Fish available in Baigul reservoir are grouped in to five categories i.e. A, B, C, D and E. Total fish landing was 2124.9 and 2044.61 quintal for the year 2006 and 2007 with the production of 70.93 kg/ha/yr and 68.27 kg/ha/yr respectively. Group C was dominant (38.68% in 2006 and 34.28% in 2007) followed by group D fishes (30.0% in 2006 and 30.44% in 2007). Landing of group A and B fishes has increased from 11.55 to 17.11% and from 6.64 to 8.35% respectively. In group A fishes highest contribution was by L. rohita followed by C. mirgala. Severe decline was recorded in landing of C. carpio from 22.33% in 2006 to 5.01% in 2007. Total fish catch was highest in June in both the years followed by in May and November to December in 2006 and November to January in 2007 indicating that some pattern in fish landing in Baigul reservoir was apparent which seems to be correlated with the fishing pattern and water depth or both. The range of water quality parameters noted for the year 2006 and 2007 from Baigul reservoir were pH (7.1- 7.8 and 7- 8.2); conductivity (120- 190 µ/cm and 115- 190 µ/cm); total dissolved solid (80- 137 mg/l and 80- 150 mg/ l); transparency (65- 180 cm and 61- 260 cm); dissolved oxygen (5.2- 7.6 ppm and 5.8- 7.5 ppm); CO2 (0- 6 ppm and 0- 8 ppm) and total alkalinity (78- 126 ppm and 85- 120.35 ppm) respectively. The r2 values were ranged from 0.57 to 0.89 for the year 2006 and 0.34 to 0.83 for the year 2007 and almost all the regressions were found significant (p< 0.001). Besides that the b values obtained for the species N. notopterus for the year 2006 was ranged between 0.058 to 3.075 and for 2007 between 0.950 to 2.794. The condition factor (CF) had shown positive significant correlation with gonadal development in male and female at different stages. Changes in CF might be correlated differently with the advancement of oocytes in ovary and spermatocytes in testis in same group or in fish of different size or age groups. Condition factor (CF) and somatic condition factor (SCF) being positively correlated in both the sexes of N. notopterus was indicative of increase of somatic component also along with rising gonadal weight. Pronounced negative correlation was observed between SCF and GSI in female N. notopterus, but was insignificant in their males. Condition factor and somatic condition factor being positively correlated in both the sexes N. notopterus was indicative was of increase of somatic component also along with rising gonadal weight. Pronounced negative correlation was observed between SCF and GSI in female N. notopterus but insignificant in males. Changes in GSI in both sexes of N. notopterus exhibited similar increasing pattern and seasonal cyclicity during both the years. Gondal development was more rapid during June, July & August to attain maturity for spawning. Lipid concentration in testis was much lower than in ovary of N. notopterus during spawning period and this could be due to fast ovarian growth and accumulation of yolk material in oocytes. The increased protein values in gonads during active reproductive phase compared to liver and muscle of N. notopterus are indicative of role of protein in gonadal development.
  • ThesisItemOpen Access
    Population genetic analyses of Labeo gonius (Hamilton,1822) in different reservoirs of Uttarakhand by using allozyme and RAPD markers
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-08) Tewari, Grishma; Singh, I.J.
    The present population genetic study was carried out to identify basic genetic structure of Labeo gonius from three different sized reservoirs of Uttarakhand viz., Dhaura, Baigul and Nanak Sagar reservoir by applying allozyme and random amplified polymorphic DNA (RAPD) markers. Allozyme analysis revealed total 19 loci out of which 11 were polymorphic (57.89%). RAPD-PCR produced total 11487 bands in overall populations by using 15 decamer operon series primers (OPA, OPB, OPC and OPY) with 32 individuals in each population. Out of total 11487 bands, 8344 bands were found to be polymorphic with a polymorphic band percentage of 72.63%. Other genetic diversity parameters were estimated by using software GENEPOP Ver.3.4 and POPGENE Ver. 3.1 for allozyme and RAPD analysis, respectively. Observed heterozygosity (Ho= 0.49-0.53), Nei’s genetic diversity (H= 0.39), Shannon’s information index (I= 0.57), total genetic diversity (Ht= 0.37) indicated most heterozygous structure in Nanak sagar population followed by Baigul and Dhaura reservoir. Values of Fst (0.106) and Gst (0.131) showed moderate genetic differentiation among the populations indicated little gene exchange with Nm value of 1.344. The allozyme and RAPD analysis in L. gonius concluded that these populations are not greatly differentiated from each other and presence of optimum level of genetic variation in Nanak sagar population in comparison to Dhaura and Baigul reservoir.