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ThesisItem Open Access Characterization of Schizothorax richardsonii stocks of different lotic water bodies of Uttarakhand using truss network analysis and microsatellite markers(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2019-01) Lohani, Vibha; Ram, R.N.Uttarakhand state has plenty of water resources in the form of rivers, streams and the glaciers of Western Himalayas make these rivers more sacred and eye-catching. Schizothorax richardsonii is an indigenous Himalayan fish species commonly called as Snow trout” due to its similarity with trout that is sharp teeth. It contributes to the cold water fishery in the Himalayan region and dwells in fast flowing snow-fed rivers (8-22°C), streams and lakes. The present study investigated the patterns of phenotypic and genotypic variations of this economically important fish. The sampling sites included four natural lotic water bodies of the state Uttarakhand and one farmed stock of the target fish i.e. Kosi River, Alaknanda River, Chirapani stream, Gaula River and captive stock of DCFR Farm, Bhimtal. Biometrics revealed that Alaknanda River showed isometric growth (b = 3.08) while the others have negative allometric growth and the condition factor was also highest in Alaknanda River (K=1.81). Phenotypic study was performed using Truss Network of 14 landmarks making 31 characters which reported high degree of significant morphometric heterogeneity among the populations with size within landmarks and shape as major factors of divergence in the selected ecosystems. Between-groups Principal Component Analysis revealed that the first and second principal components accounted for 91.2% and 5.4% of variance respectively. 94.5% of original grouped cases were correctly classified each original group for morphometric measurements of S. richardsonii and formed separate clusters supporting the fact that environment plays a vital role in shaping the phenotypic characters. Inter- population microsatellite assay analyzed that mean of observed number of alleles were 5.20, mean of effective number of alleles were 3.50, Observed heterozygosity was 0.57, expected heterozygosity was 0.63, Shannon’s Information Index was 1.40, Fixation Index (FST) was 0.12 and gene flow (Nm) was 1.83. The results revealed a low molecular variance of 13% among populations, 3% of difference within populations and 84% of molecular variance within individuals. The genotypic results suggested moderate level of genetic diversity among populations. The complimentary study of phenotypic and genotypic variations is an attempt of stock assessment of Snow Trout in Uttarakhand for the rational exploitation, conducting conservation and sustainable management measures.