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ThesisItem Open Access Effect of mode of administration on hematological parameters and biochemical profile of Labeo rohita (Ham.) fingerlings exposed to mercuric chloride(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-08) Bhatt, Pooja; Ram, R.N.The present investigation was aimed at to find out the mode of administration dependent variation in hematological parameters (Hb level, TEC, TLC, DLC, Ht percent, MCV, MCH and MCHC) and biochemical profile (Moisture, Protein, Lipid, Ash and NFE) of Labeo rohita fingerlings when they were exposed to HgCl2. The experiment was performed in two phases, acute and chronic. For acute toxicity assay Labeo rohita fingerlings were exposed to different doses of mercuric chloride at the rate of 0.6, 0.4 and 0.2 mg/l through water and 0.6, 0.4 and 0.2 mg/kg through feed for 96 hrs. In a different set of acute toxicity experiment, different sizes of Labeo rohita viz. 18mg, 8.5mm; 49mg, 18mm and 220mg, 40mm were exposed to a single dose i.e. 0.1 mg/l through water and 0.1 mg/kg through feed for 168 hrs. For chronic toxicity assay, Labeo rohita fingerlings were exposed to HgCl2 for 49 days both via water (0.05mg/l) and via feed (0.05mg/kg feed) and assessment of different hematological parameters and biochemical profile was done on 0th, 7th, 21st and 49th day. The results of acute toxicity assay revealed that the hematological parameters and biochemical profile were affected by dose, size and mode of administration. Younger fishes were more vulnerable to mercuric chloride and fishes showed major responses toward higher doses. Significant differences were observed in the mode of administration dependent variation in hematological and biochemical parameters except in muscle protein (96hrs exposure) and in basophil count, MCHC, moisture and NFE (168hrs exposure). Chronic exposure assay established that the dietary mercuric chloride pose more health hazard to fish than the mercuric chloride administered through water.ThesisItem Open Access Limnological profile and ichthyofaunal diversity of Nanaksagar reservoir(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-06) Raveendar, Banothu; Sharma, A.P.Present study was conducted to assess the limnological characteristics and ichthyofaunal diversity in Nanaksagar reservoir, located in Tarai region of Uttarakhand. Along with soil and water quality parameters, analysis of phytoplankton, zooplankton and fish communities was done in Nanaksagar reservoir. Monthly samplings were conducted at three selected sites during August 2016 to March 2017. A total number of 24 species of phytoplankton, 14 species of zooplankton and 30 species of fishes were recorded during the study period. Maximum population of phytoplankton (2.31x 105 cells/l) and zooplankton (14.0 x103 individuals/l) occurred in the month of February 2017. The fish data is subjected to species diversity analysis. Among fishes, Gadusia chapra with mean annual contribution of 44.14% was the most dominant fish species in the catches. Labeo gonius with a contribution of 15.95% was another quantitatively dominant species during the study period. Among Indian major carps Cirrhinus mrigala was the most abundant. The Cat fishes and minor carps were also present in appreciable numbers in the fishery of the Nanaksagar reservoir. Maximum and minimum fish catch was obtained in the month of February, 2017 and September, 2016 respectively. As per values of Shannon (2.118 – 2.612) and Simpson diversity (0.822 – 0.8954) indices fish community of the reservoir is diverse. The range of Margalef and Menhinick species richness indices (3.253 – 3.782 and 0.9183 – 1.065, respectively) indicates moderate fish species richness. Fish production was found to be positively correlated with total alkalinity, dissolved oxygen, zooplankton, phytoplankton and pH.ThesisItem Open Access Assessment of fisheries potential using geospatial technology in selected reservoirs of Tarai region of Uttarakhand(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2016-01) Ingole, Nandkishor Atmaram; Ram, R.N.Present study deals with application of geospatial technology for discrimination of different physico-chemical parameters, seasonal variation of plankton, present status of fish fauna, demarcation of productive zone and site suitability for culture practices of selected reservoirs (Nanak Sagar and Tumaria) in Tarai region of Uttarakhand. Both the reservoirs are having different catchment area and environmental conditions. Study was conducted for a period of 12 months (seasonal variation) from July, 2014 to June, 2015. Each time twenty sampling points of same coordinates (every time) were selected with the help of GPS. The physico-chemical parameters of water and soil were analyzed from selected reservoirs and annual range of these parameters was worked out. It showed less variation in Nanak Sagar as compared to Tumaria reservoir that may be due to presence and absence of floating weed Salvinia. Seasonal variation of phytoplankton showed that more number of species are found in Nanak Sagar (28) than Tumaria reservoir (23). Annual range of phytoplankton varied from 69253-113904 no/l and 65229-136941 no/l in Nanak Sagar and Tumaria reservoir, respectively. The qualitative and quantitative analysis of phytoplankton showed presence of 39% species of Chlorophyceae, 36% species of Bacillariophyceae and 25% species of Cyanophyceae in Nanak Sagar and 39%, 35% and & 26% in Tumaria reservoir. The total zooplankton variation 1977-2779 no/l of 19 and 1895-3479 no/l of 18 species in Nanak Sagar and Tumaria reservoir, respectively. Five species of Cladocera and Copepod each have been found in both reservoirs, whereas 9 species of Rotifer group has been recorded in Nanak Sagar and 8 species are in Tumaria reservoir. The thematic maps of annual distribution of each water and soil parameters and seasonal variation of planktons were also created. Total annual catch of 274225 Kg and 64808 Kg were estimated in year 2014-15 from Nanak Sagar and Tumaria reservoir, respectively. It is concluded that average fish production (124.60 kg/ha) of Nanak Sagar was more than average production (40 kg/ha) of Tumaria reservoir, mainly because of lesser productive water and Salvinia infestation in Tumaria reservoir. GIS technique has been used to demarcate the productive zone in the selected reservoirs. The results indicate that high productive zone 15.34% area in Nanak Sagar and 10.19% area in Tumaria reservoir. The suitable sites for cage culture were also identified which were approximately 40.90% in Nanak Sagar and 43.18% in Tumaria reservoir. Using GIS technique 15.34% and 10.19% area out of total area were classified as highly suitable for pen culture system in Nanak Sagar and Tumaria reservoir, respectively. Spectral model of hydrological parameters and phytoplankton biomass of both reservoirs were also prepared. Accuracy assessment based on predictive and observed value was carried out on the basis of co-efficient of determination (R2). The higher accuracy in all spectral models of water quality parameters and phytoplankton biomass of Nanak Sagar as compared to Tumaria reservoir might be due to Salvinia infestation in Tumaria reservoir. Results of this study are promising and can be used to conclude that selected reservoirs have immense fisheries potential. It is recommended that the geospatial technologies may be used to study the future impact of human interventions.