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  • ThesisItemOpen Access
    Effect of mode of administration on hematological parameters and biochemical profile of Labeo rohita (Ham.) fingerlings exposed to mercuric chloride
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-08) Bhatt, Pooja; Ram, R.N.
    The present investigation was aimed at to find out the mode of administration dependent variation in hematological parameters (Hb level, TEC, TLC, DLC, Ht percent, MCV, MCH and MCHC) and biochemical profile (Moisture, Protein, Lipid, Ash and NFE) of Labeo rohita fingerlings when they were exposed to HgCl2. The experiment was performed in two phases, acute and chronic. For acute toxicity assay Labeo rohita fingerlings were exposed to different doses of mercuric chloride at the rate of 0.6, 0.4 and 0.2 mg/l through water and 0.6, 0.4 and 0.2 mg/kg through feed for 96 hrs. In a different set of acute toxicity experiment, different sizes of Labeo rohita viz. 18mg, 8.5mm; 49mg, 18mm and 220mg, 40mm were exposed to a single dose i.e. 0.1 mg/l through water and 0.1 mg/kg through feed for 168 hrs. For chronic toxicity assay, Labeo rohita fingerlings were exposed to HgCl2 for 49 days both via water (0.05mg/l) and via feed (0.05mg/kg feed) and assessment of different hematological parameters and biochemical profile was done on 0th, 7th, 21st and 49th day. The results of acute toxicity assay revealed that the hematological parameters and biochemical profile were affected by dose, size and mode of administration. Younger fishes were more vulnerable to mercuric chloride and fishes showed major responses toward higher doses. Significant differences were observed in the mode of administration dependent variation in hematological and biochemical parameters except in muscle protein (96hrs exposure) and in basophil count, MCHC, moisture and NFE (168hrs exposure). Chronic exposure assay established that the dietary mercuric chloride pose more health hazard to fish than the mercuric chloride administered through water.
  • ThesisItemOpen Access
    Limnological profile and ichthyofaunal diversity of Nanaksagar reservoir
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-06) Raveendar, Banothu; Sharma, A.P.
    Present study was conducted to assess the limnological characteristics and ichthyofaunal diversity in Nanaksagar reservoir, located in Tarai region of Uttarakhand. Along with soil and water quality parameters, analysis of phytoplankton, zooplankton and fish communities was done in Nanaksagar reservoir. Monthly samplings were conducted at three selected sites during August 2016 to March 2017. A total number of 24 species of phytoplankton, 14 species of zooplankton and 30 species of fishes were recorded during the study period. Maximum population of phytoplankton (2.31x 105 cells/l) and zooplankton (14.0 x103 individuals/l) occurred in the month of February 2017. The fish data is subjected to species diversity analysis. Among fishes, Gadusia chapra with mean annual contribution of 44.14% was the most dominant fish species in the catches. Labeo gonius with a contribution of 15.95% was another quantitatively dominant species during the study period. Among Indian major carps Cirrhinus mrigala was the most abundant. The Cat fishes and minor carps were also present in appreciable numbers in the fishery of the Nanaksagar reservoir. Maximum and minimum fish catch was obtained in the month of February, 2017 and September, 2016 respectively. As per values of Shannon (2.118 – 2.612) and Simpson diversity (0.822 – 0.8954) indices fish community of the reservoir is diverse. The range of Margalef and Menhinick species richness indices (3.253 – 3.782 and 0.9183 – 1.065, respectively) indicates moderate fish species richness. Fish production was found to be positively correlated with total alkalinity, dissolved oxygen, zooplankton, phytoplankton and pH.