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20122
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Subject: Clothing and Textiles × Author: Bhargava, Deepti × End date: 2024 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Microbial dyes from Pseudomonas fluorescens and Pseudomonas aeruginosa: extraction and optimization of dyeing process for textiles
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2012-12) Bhargava, Deepti; Shahnaz Jahan
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    ThesisItemOpen Access
    Microbial dyes from Pseudomonas fluorescens and Pseudomonas aeruginosa: Extraction and optimization of dyeing process for textiles
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2012-12) Bhargava, Deepti; Shahnaz Jahan
    The world is shifting from synthetic to nature based products in today’s era. The recent realization that the intermediates and chemicals used in synthetic dyes are toxic, and thus hazardous to human health and environment, has led to the revival of interest in the non- toxic and eco-friendly natural dyes. Emphasis has been given to identify new sources of raw materials to be used as a dye and to standardize the procedures for their use. Therefore, present research work was carried out with the objectives to optimize the growth conditions of Pseudomonas fluorescens (Pf-6) and Pseudomonas aeruginosa (Pa), to optimize the dyeing variables, to see the effect of synthetic and natural mordants with dye using different mordanting methods, to assess the color fastness of dyed fabric, to test the antimicrobial activity and toxicity of the selected dye sources. Maximum pigment was extracted from Pseudomonas fluorescens (Pf-6) when it was inoculated on King’s B agar at 7pH and incubated for two to seven days at 250C in BOD incubator. In case of Pseudomonas aeruginosa (Pa), maximum pigment can be extracted when it was inoculated in King’s B broth at 7pH and incubated for two to seven days at 250C in incubator shaker. Best colour was obtained on silk fabric when it was dyed with Pseudomonas fluorescens (Pf-6) dye keeping the pH 5 of the dye solution, 2 percent concentration of the dye, 1:30 material to liquor ratio and dyed at 800C for 75 minutes. Similarly, cotton fabric was dyed with Pseudomonas fluorescens (Pf-6) dye keeping the pH 5 of the dye solution, 2 percent concentration of the dye, 1:30 material to liquor ratio and dyeing was done at 800C for 90 minutes in order to obtain best colour. Each silk and cotton fabric was dyed with Pseudomonas aeruginosa (Pa) dye with the optimum dyeing variables including pH 7 of the dye solution, 1 percent concentration of the dye, 1:50 material to liquor ratio and dyed at 800C for 75 minutes for obtaining best colours. Best colours on silk fabric were obtained when they were mordanted with each mordant i.e. 3 g bahera, 4 g walnut bark, 0.02 g ferrous sulphate and 0.03 g tannic acid along with Pseudomonas fluorescens (Pf-6) dye. Similarly, best colours on cotton were obtained when it was mordanted with each mordants i.e. 4 g walnut bark, 3 g pomegranate rind, 0.02 g ferrous sulphate and 0.02 g alum in combination with Pseudomonas fluorescens (Pf-6) dye. In case of Pseudomonas aeruginosa (Pa) dye, best colours on silk fabric were developed when it was mordanted with each 3 g of harad and walnut bark and 0.03 g of each tartaric acid and tannic acid. Similarly, 4 g of each bahera and pomegranate rind and 0.04 g of each alum and tannic acid was used along with Pseudomonas aeruginosa (Pa) dye to obtain best colours on cotton fabric. Overall colour fastness properties of both the dyes were improved with the use of natural and synthetic mordants. Both the dyes were found to have antimicrobial properties and non toxic in nature.