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Subject: Chemistry × Author: Arya, Sureshkumar Premram × End date: 2019 × Start date: 2013 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Phytochemical analysis and biological activity determination of Zingiber roseum Rosc
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2016-07) Arya, Sureshkumar Premram; Om Prakash
    Zingiber roseum was collected from Padampuri, Uttarakhand in the month of September-October, 2015. The essential oil was isolated from fresh seeds by hydrodistillation using Clevenger’s type apparatus. Essential oil and all extracts were analysed by GC-MS for their phytochemical composition. Extracts of seeds, rhizome and perianth obtained in organic solvent of different polarity were for their antifungal activity against two phytopathogenic fungi viz; Rhizoctonia solani and Fusarium accuminata, antioxidant assay by different methods viz; reducing power activity, chelating activity of Fe2+, DPPH radical scavenging activities. All the methanolic extracts were studied for their qualitative analysis of phenolics by HPLC and herbicidal activity on Raphanus sativus (radish). The dried and powdered seeds, rhizomes and perianth were studied for their mineral profiling with help of Atomic Absorption Spectroscopy (AAS). The GC and GC-MS analysis revealed the presence of β–cubene (13.4%), caryophyllene (12.6%) and neryl linalool isomer (24.06%) were identified as major compounds compounds in oil. However neryl linalool isomer, geranyl linalool isomer, β-citronellal, 9-octadecenoic acid, l-(+)-ascorbic acid 2,6dihexadecanoate, 2-(4-hydroxy-3-methoxyphenyl)-3,7-dimethoxy-4h-chromen-4-one, 9,12-octadecadienoic acid (Z,Z)-, methyl ester and hexadecanoic acid, methyl ester were found major compounds in different extracts. HPLC analysis revealed the presence of ferullic acid, gallic acid and vanillic acid in different parts of plant. Extracts exhibited good to moderate antioxidant activity in dose dependent manner. Among all the extracts, Z. roseum perianth methanolic extract (ZRPME) possessed maximum DPPH radical scavenging activity; Z. roseum seed methanolic extract (ZRSME) showed maximum reducing power activity and maximum metal chelating activity was shown by Z. roseum perianth hexane extract (ZRPHE). Quantative analysis of total phenols, orthodihydric phenols and flavonoids ranged from (0.16±0.0 to 4.5±0.0 mg/g GAE), (0.01±0.0 to 4.4±0.0 mg/g CLE) and (11.5±0.0 to 78.2±0.01 mg/g CNE) in different extracts respectively. All extracts were found to be active against both fungi. All methanolic extracts inhibited the fungal mycelial growth of F. accuminata, while the extracts also possessed fungal growth promoting against R. solani. Z. roseum seed methanolic extract (ZRSME) was found most effective for inhibiting the growth of fungus. The extracts inhibited and promoted mycelial growth in respective fungi in dose dependent manner. The methanolic extract of Z. roseum were found to be active and inhibited the germination in the seeds of R. sativus (radish) and Z. roseum rhizome methanolic extract ZRRME showed more seed germinating activity than Z. roseum seed methanolic extract (ZRSME). Elemental analysis of various parts revealed the presence of Fe, Mn, Zn, Cu, Mg, Cd, Cr, N, P and K in varying quantities. Based on the above results it can be concluded that Zingiber roseum can be a good source of natural antioxidant, anti-fungal and herbicide after proper clinical trials besides its academic importance.