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Subject: Chemistry × Author: Arya, Stuti × End date: 2024 × Start date: 2020 × Type: Thesis ×
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    ThesisItemOpen Access
    Seasonal variation in chemical composition and biological activities of essential oils from leaves of Skimmia anquetilia N.P. Taylor and Airy Shaw
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-10) Arya, Stuti; Om Prakash
    The aim of the current investigation was to analyze the chemical composition and biological activities of the essential oil from the aerial part of Skimmia anquetilia (N.P Taylor and Airy Shaw). The plant material was collected was from Harinagar, District Nainital, Dhari, Uttarakhand India, situated at an altitude of 2100m. during the month of September 2020 and January 2021. The essential oil were extracted by hydro-distillation method using Clevenger type apparatus and were designated as SKREO (Skimmia anquetilia rainy essential oil) and SKWEO (Skimmia anquetilia rainy essential oil) rescpectively. The yield of SKREO & SKWEO were 0.70% and 0.60% respectively by v/w. The essential oils were analysed for phytochemical composition and biological activity viz, antioxidant, anti-inflammatory, antidiabetic, antifeedant and nematicidal activity. GC and GC/MS analysis of SKREO & SKWEO was done for qualitative and quantitative analysis viz: linalyl acetate (15.8% -17.6%), linalool (13.2%- 13.9%), geijeren (11.6%- 11.7%), thujene (11.3%- 11.1%), α-terpineol (6.1%- 6.1%), geranyl acetate (5.0%- 5.1%), α–terpinyl acetate (3.3%- 3.1), myrcene (3.0%- 3.1%), geraniol (2.6%- 1.9%), α-pinene (2.1%- 2.2%), transβ- ocimene (2.1%- 2.3%), cis-β-ocimene (2.0%- 2.2%), neryl acetate (2.3%- 2.4%), sabinene (1.4%-1.5%), α- elemol (1.8%- 1.3%), epi- α -elemol (1.9%- 1.6%), 3- farnesyl acetate (1.7%- 2.4%) respectively. In terms of quality many constituents identified in SKREO were minor in SKWEO and viceversa. The in vitro antioxidant activity of SKREO & SKWEO was conducted by means of DPPH radical scavenging, H2O2 radical scavenging and metal chelating activity taking BHT and Na2 EDTA as the antioxidant standard respectively. SKREO showed higher DPPH radical scavenging potential (IC50=36.91±0.397) than SKWEO (IC50=41.31±0.561). In H2O2 radical scavenging assay SKREO showed great activity while in metal chelating activity SKREO showed better antioxidant potential than SKWEO. The in vitro anti-inflammatory activity was conducted using protein denaturation assay taking standard dichlofenac sodium as an anti-inflammatory drug. SKWEO (IC50=43.77±0.346) showed strong in vitro anti-inflammatory activity than SKREO (IC50=47.83±0.104). The antidiabetic activity of SKREO & SKWEO was performed using α-amylase inhibition assay by taking standard antidiabetic drug acarbose. SKREO (IC50=38.210±.030) showed better antidiabetic activity than that of SKWEO (IC50=52.06± 0.045 μl/mL). Antifeedant activity of both SKREO & SKWEO was performed against Spodoptera litura insect and the observation showed both SKREO (74.06%) and SKWEO (91.67 %) were observed to exhibited potent antifeeding activity at dose dependent manner. The nematicidal mobility activity of SKREO & SKWEO was performed against Meloidogyne incognita. The results revealed that SKREO was found to be most effective with 48.66% and 47.41% in SKWEO. SKREO gave minimum inhibition of egg hatching with 6.43% as compared to SKWEO 11.27%. The shrub S.anquetilia can be good source of phytochemicals like linalyl acetate, linalool, geijeren, thujene and herbal source for antioxidant, antiinflammatory, antidiabetic, antifeedant and nematicidal activity after proper clinical trials, besides its academic importance.