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Subject: Botany × Author: Kashyap, Shakuli × Start date: 2013 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Antioxidant profiling and activity in some important medicinal plant species of Uttarakhand
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2014-08) Kashyap, Shakuli; Rao, P.B.
    Traditional herbal medicines form an important part of the healthcare system of India. Ayurveda is the oldest medical system in the world, provides potential leads to investigate active and therapeutically useful compounds from medicinal plant species. Considering the growing interest to ascertain the potential of medicinal plant species as important sources of natural antioxidants, the present study was conducted on 54 different plant parts sampled from 43 different selected important medicinal plant species growing in MMPG/MRDC, G.B.P.U.A. & T., Pantnagar or generally obtained in Uttarakhand state. Antioxidant profiling and activity in aqua-methanol (30:70, v/v) extracts in different plant species were performed by using three different assays namely, 2, 2-diphenyl-1-1picrylhydrazyl (DPPH) free radical scavenging, ferrous ion (Fe2+) chelating ability (FCA) and ferric reducing/antioxidant power (FRAP); non enzymatic viz., total phenolic and flavonoid content; and enzymatic antioxidants viz., superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were analyzed in whole aerial parts (8); flowers (7); Fruits (4); seeds (2); roots (2); bark (4); and leaves (28). Comparatively higher values were found in M. pudica, O. gratissimum, T. bellirica, A. precatorius, R. serpentina, T. arjuna and Q. indica in DPPH (%); A. paniculata, C. procera, T. bellirica, S. cumini, R. serpentina, B. variegata, H. rosa-sinensis in FCA (%); E. alba, O. gratissimum, T. chebula, S. cumini, R. serpentina, T. arjuna and E. officinalis in FRAP (VCE or TE μg/mg extract); P. niruri, O. gratissimum, T. bellirica, A. precatorius, R. serpentina, T. Arjuna and B. variegata in TPC (μg GAE/mg extract); A. annua, A. annua, T. chebula, S. cumini, R. serpentina, B. variegata and B. ceiba in TFC (μg QE/mg extract); M. pudica, O. gratissimum, T. chebula, S. cumini, R. serpentina, T. arjuna and P. roxburghii in SOD (U/mg FW); B. monnieri, C. procera, T. chebula, S. cumini, A. racemosus, T. arjuna and E. officinalis in CAT (U/mg FW); and A. paniculata, O. gratissimum, T. chebula, S. cumini, R. serpentina, T. arjuna and I. coccinea in POD (U/mg FW) than other species. Thus, the above results provide scientific basis for the use of these medicinal plant species as folk remedies, promising source of natural antioxidants for the prevention and treatment of different diseases caused by oxidative stress.