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Subject: Botany × Author: Bahuguna, Aabha × End date: 2024 × Start date: 2020 × Type: Thesis ×
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    ThesisItemOpen Access
    Media optimization for Fusarium pallidoroseum, a novel biofertilizer
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-11) Bahuguna, Aabha; Sharma, A.K.
    Food is the main source for humans and development of nation is directly proportional to the amount of food or nutrients available to the population. The growing population and limited cultivation land demand the application of fertilizer to meet the human needs. Because of its vast potentiality in increasing crop productivity and food safety, the application of microorganisms as biofertilizer is seen as potential alternative to chemical fertilizer. Though Arbuscular Mycorrhizal Fungi (AMF) are recognized as a distinctive biofertilizer yet they are difficult to mass multiply since they are biotrophs, and their growth under axenic conditions has proved to be impossible. Therefore, a newly described axenically cultivable endophytic and saprophytic fungus Fusarium pallidoroseum, a novel biofertilizer which has a substantial favourable impact on plant growth promotion has been discovered. There is a little information about the culture media used for the production of spores. Therefore, our current study was carried out to optimize submerged fermentation medium for Fusarium pallidoroseum and evaluating highest spore producing cultivation media composition by Plackett Burmann Design (PBD) and Response Surface Methodology (RSM). The PBD selected 6 significant medium composition out of the 10 screened. Among which three significant factors Peptone (P value of < 0.0001 and F = 81.14), K2HPO4 (P value of < 0.0003 and F value = 28.42) and trace metals (P value of < 0.0001 and F value of 39.39) were chosen for RSM to produce higher spore production. Therefore, an optimized cultivation media 0.0592 (g/L) K2HPO4, 0.0531 (g/L) MgSO4, 0.113 (g/L) CaCl2, 25.6 (g/L) Sucrose, 0.03 (g/L) Ferric Ammonium citrate, 0.002 (g/L) EDTA, 0.063 (g/L) NaCl, 2 (ml/L) Trace metals, 0.434 (g/L) Na2HPO4, and 6 (g/L) Peptone was obtained through PBD and RSM.