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20221
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Subject: Biotechnology and molecular Biology × Author: Arya, Mamta × End date: 2022 × Type: Thesis ×
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    ThesisItemOpen Access
    Genetic diversity, phytochemical and anticarcinogenic studies in cichorium intybus from Himalayan elevations of Uttarakhand
    (G. B. Pant University of Agriculture and Technology, Pantnagar, 2022-08) Arya, Mamta; Gohar Taj
    Chicory (Cichorium intybus L.) comprise of intense therapeutic and medicinal properties due to presence of phytochemical and secondary metabolites. It is often used as a medication in India to treat a number of illnesses such as fever, diarrhea, spleen enlargement, gout, and rheumatism. The purpose of this study was to determine the genetic diversity of chicory from Himalayan regions of Uttarakhand using SSR markers. So, it will be entrancing to know about the genetic diversity of chicory and if any genotypes are deficient in medicinal content then it could be enhanced through various molecular interventions. The cluster analysis of 100 genotypes grouped it into two clusters A and B, in which cluster A comprised of 94 genotypes. The other B cluster consists of 6 genotypes. Additionally, to investigate the genetic constitution of diverse 100 chicory genotypes genotyping by sequencing was performed, population structure and genetic diversity analysis were also done. Cluster analysis using neighbor joining method grouped the population into 3 clusters ‘A’, ‘B’ and ‘C’ depending on their geographical location. The main cluster ‘C’ consists of 61 genotypes, cluster B comprised of 28 genotypes and cluster A comprised of 11 genotypes. From GBS technique thousands of SNPs identified were used for the association mapping to recognize authentic markers linked to the agro-morphological traits of Cichorium intybus plant. There were 178 SNPs markers showed associations with the eight agromorphological traits at significant threshold level. Further, to study the difference in the phytochemical constituents quantity of chicory, qualitative and quantitative analysis were performed among 10 genotypes and identified that genotypes showed considerable amount of variation. Lactucine the phytochemical constituents of chicory also have anti-cancer properties and can treat various types of cancers such as human leukemia cancer. In present investigation, lactucine and its derivatives are docked with the apoptosis inducing and cell cycle progression proteins for the prediction of its anticancer effect. Among 17 isoforms of lactucine, Lactucin 15- oxalate has shown highest binding affinity with target protein with appropriate Glide and Dock score. These in silico results are valuable to proceed for the in vitro and in vivo studies related toanti-cancer role of lactucin 15-oxalate. Therefore, chicory plant has high in its diversity can be considered as a high value herbs for the medicinal purposes and even its phytochemical content are also enhancing nutritional values. The identified SNPs with the agro-morphological trait indicate its high rate of association which is useful in improving the medicinal properties. Even, due to anticancer effect of lactucinee can be used for treatment of leukemia cancer.