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Subject: Biotechnology and Molecular Biology × Author: Shinde, Sagar Sharad × End date: 2017 × Start date: 2017 × Type: Thesis ×
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    ThesisItemOpen Access
    Molecular mechanisms underlying the stress protective effect of zinc sulfide nanoparticles in Brassica juncea
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-08) Shinde, Sagar Sharad; Arora, Sandeep
    Application of nanoscience and technology in agriculture have provided a ray of hope for increasing crop productivity in a sustainable manner. In the current project, efforts were made to find out whether treatment of Brassica juncea, an important oilseed crop, with zinc sulfide nanoparticles can prepare them to withstand the impeding water deficit stress and what are the molecular mechanisms associated with the effect of these nanoparticles. Zinc sulfide nanoparticles, synthesized through chemical reduction method, were characterized through UV-visible spectroscopy. Brassica juncea seedlings were treated with different concentrations of zinc sulfide nanoparticles (0, 5, 15, 25, 50 & 100ppm) and one set of seedlings was subsequently subjected to 2-days of water deficit stress, and the other set was maintained under regular irrigation conditions. It was observed that seedlings pre-treated with 15 & 25ppm of nanoparticles were able to withstand the water deficit stress better than the untreated seedlings. The levels of standard stress marker molecules were significantly lower in the seedlings pre-treated with zinc sulfide nanoparticles, as compared to the untreated seedlings. Further a differential expression response of various antioxidant genes of Halliwell-Asada pathway was recorded in the pre-treated seedlings, indicating that the stress protective effect of zinc sulfide nanoparticles was mediated through the improved redox status of the pre-treated seedlings. An analysis of the expression profile of specific mapk genes indicated that mapk3 and mapk9 play a major role in mediating the effect of zinc sulfide nanoparticles.