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Subject: Biochemistry × Author: Munagapati, Sandhya × End date: 2009 × Start date: 2004 × Type: Thesis ×
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    ThesisItemOpen Access
    Biochemical evaluation of genotypes of Jatropha curcas L., and optimization of micropropagation schedule
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2005-07) Munagapati, Sandhya; Gaur, A.K.
    Five genotypes of Jatropha curcas viz. shu03001, shu03002, shu03004, shu04001 and shu04004 were selected for their further identification and biochemical characterization. Identification of genotypes had been done on the basis of banding pattern on SDS-PAGE of total seed storage protein and its fractions viz. globulins and albumins. Based on the Jaccard‟s similarity coefficient obtained from the banding patterns of SDS-PAGE, the five genotypes are grouped into two categories. Group1 consisting of shu03001, shu03004 and shu04004 and Group2 consisting of shu03002 and shu04001. These results were based in accordance with the percentage protein content of the respective genotypes taken for study and the oil contents of these genotypes were almost comparable. However, the lipid/protein ratio was highest in shu04001. In view of optimizing micropropagation schedule, the experiments were also conducted on three genotypes of J.curcas viz. shu03001, shu03002 and shu03004 to study the effect of various parameters viz. genotypes, growth regulators and explants and their interactions on callus induction frequency. Two explants viz., leaf and petiole from all the three genotypes were placed on different combinations of growth regulators. Calluses were induced from the two explants on all types of callus induction media containing four levels of 2,4-D (1, 2, 2.5and 3mg/l) with BAP (0.1,0.2,0.25 and 0.3mg/l) and two levels of NAA (1 and 2 mg/l) with two levels of BAP (0.1 and 0.2 mg/l). Maximum callus induction frequency of 92.8, 93.1; 97.4, 98.7 and 88.8, 96.1% was observed for leaf and petiole explants of shu03001, shu03002 and shu03004 genotypes respectively. In a generalized way, it was observed that shu03004 genotype showed highest callus induction frequency followed by shu03002 and shu03001. Media with NAA (1mg/l) and BAP (0.1mg/l) showed highest callus induction frequency with both leaf and petiole explants in all the three genotypes. Amongst explants, petiole showed higher callus induction frequency followed by leaf explant on all types of callus induction media.